| Strain Descriptor |
Strain Summary |
Genotype |
Phenotype |
| mcln- | mcln null mutant expressed in DH1-10 cells | axeA1, axeB1, axeC1, pyr5-6- [pRG24], ura-, mcln-, bsr | |
| mhcA-/[act15]:mhcA(F481V) | mhcA(F481V) expressed in mhcA null
| axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, thy+, mhcA-[pTZD1THY], mhcA(F481V), [pBIG], neoR | |
| myoB-/[act15]:GFP:myoB(1-921 | myoB(1-921,1057-1111)-missing the F-actin binding domain-expressed in myoB null | axeA2, axeB2, axeC2, myoB-, [bsRcas], myoB(1-921, 1057-1111), [pTX-GFP], bsR, neoR | |
| pinA- | pinA null mutant in AX2 | axeA2, axeB2, axeC2, pinA-, [bsR-loxP], bsR | |
| tgrB1-/tgrC1-/(QS31|tgrB1/tgrC1)/tgrB1(1-827):HA/[act15] :RFP | merodiploid strain with an additional tgrB1 allele of AX4 with C-terminal truncation (828-902) and HA tag in tgrB1/tgrC1(QS31) double gene replacement background; tdTomato (RFP) is expressed under the act15 promoter | axeA1, axeB1, axeC1, pyr5-6-[pRG24], tgrB1-, tgrC1-, QS31/tgrB1, trgrC1, AX4/tgrB(1-827):HA, RFP, [act15:tdTomato], ura-, bsR, hygR | |
| tgrB1-/tgrC1-/(QS31|tgrB1/tgrC1)/tgrB1:HA/[act15] :RFP | merodiploid strain with an additional tgrB1 allele of AX4 with C-terminal HA tag in tgrB1/tgrC1(QS31) double gene replacement; tdTomato (RFP) i is expressed under the actin15 promoter | axeA1, axeB1, axeC1, pyr5-6-[pRG24], tgrB1-, tgrC1-, QS31/tgrB1, trgrC1, AX4/tgrB:HA, RFP, [act15:tdTomato], ura-, bsR, hygR | |
| tgrB1-/tgrC1-/(QS4|tgrB1/tgrC1)/tgrC1 | merodiploid strain with an additional tgrC1 allele of AX4 in tgrB1/tgrC1(QS4) double gene replacement background | axeA1, axeB1, axeC1, pyr5-6-[pRG24], tgrB1-, tgrC1-, QS4/tgrB1, trgrC1, AX4/tgrC1, ura-, bsR | |
| tgrB1-/tgrC1-/QS31|tgrB1/tgrC1/QS4|tgrC1 | merodiploid strain with an additional tgrC1 allele of QS4 in tgrB1/tgrC1(QS31) double gene replacement background | axeA1, axeB1, axeC1, pyr5-6-[pRG24], tgrB1-, tgrC1-, QS31/tgrB1, trgrC1, QS4/tgrC1, ura-, bsR | |
| [act15]:acbA(Y72A) | AbcA with a Tyr to Ala substitution at position 72 expressed in AX4 cells under control of an actin 15 promotor | axeA1, axeB1, axeC1, act15:acbA(Y72A), [pDNeoII], neoR | |
| [csaA]:csaA | CsaA over expressed in AX2 under control of csaA promotor | axeA2, axeB2, axeC2, csaA, [pDDC], neoR | |
| γS13 | Radiation-sensitive mutant. | radB13 | radS |
| γS14 | Radiation-sensitive mutant. | radD14 | radS |
| γS18 | Radiation-sensitive strain. | radH18 | radS |
| γS20 | Radiation-sensitive strain. | radA18 | radS |
| γS21 | Radiation-sensitive mutant. | radF21 | radS |
| γs5 | Radiation-sensitive strain. | radG5 | radS |
| 1C7 | gp130 null mutant | axeA1, axeB1, axeC1, pyr5-6-[pRG24], ura+, gp130-[gp130/pyr5-6] | |
| 2A+/scrA/dis-Scar-GFP | Expresses Scar-GFP under the control of the discoidin promoter. Control strain is 2A+pVGct | axeA1, axeB1, axeC1, cycA1, tsgA1, acrD369, ebrB364, sprH351, manA1, tmpA600, thy-, [scrA-bsr], bsR, [pVGctSCAR], neoR | axe, thy-, cycR, acrR, tsg, bsR, neoR |
| 2A+/scrA/[dis]:GFP | Control for Scar expression studies. 2A+ is a BLAST insert into HPS400 that did not go into scrA as intended. So is a non-homologous control. pVGct is the base vector, derived from pVEII, that expresses GFP under discoidin regulation. Used to tag constructs with GFP at the C-terminus. (source of name is pVEII+GFP at the C-terminus) | axeA1, axeB1, axeC1, cycA1, tsgA1, acrD369, ebrB364, sprH351, manA1, tmpA600, thy-, [scrA-bsr], [pVGct], bsR, neoR | |
| 2AScar+ | | 2AScar+, bsR | |
| 357AP | HUD1357 cotransformed with plasmid expressing pdsA from its aggregation promoter (pGPA) and pB10TP. | pdsA552, axeA, axeB, axeC, [pGPA+pPB10TP], neoR | axe, neoR |
| 3BScar- | | 3BScar-, [pVGctppl], bsR, neoR | |
| 3BScar- | | 3BScar-, [pVGctSHD], bsR, neoR | |
| 3KO 60BAS | CF60B antisense in triple KO | axeA1, axeB1, axeC1, CF50-[ ], ctnA-[ ], CF45-[ ], [CF60B-AS], neoR | axe,neoR |
| 3KO 60BOE | CF60B overexpressor in triple KO | axeA1, axeB1, axeC1, CF50-[ ], ctnA-[ ], CF45-[ ], [CF60B-OE], neoR | axe,neoR |
| 50cre/ctncre | Double knockout of 2 counting factor components. The bsr cassettes were removed with the cre-lox system. | axeA1, axeB1, axeC1, CF50-[ ], ctnA-[ ] | axe |
| 5NT- | 5''''-nucleotidase null mutant.
Aggregates with multiple tips.
Could not be recovered from lyophilized spores. | axeA1, axeB1, axeC1, 5NT-[5NT-bsr], bsR | axe, bsR |
| 7E- | CRISPR/Cas9 null mutant of 7E; contains a 19bp deletion in the 7E gene | axeA1, axeB1, axeC1, 7E- | |
| Aγ3 | Radiation-sensitive mutant in AX3. | axeA1, axeB1, axeC1, radI24 | axe, radS |
| aarA- | aarA (aardvark) null mutant (β-catenin knock-out) | axeA2, axeB2, axeC2, aarA-[aarA-KO], bsR | evil twin phenotype, axe, bsR |
| aarA-/[act15]:GFP-mhcA | aarA null mutant expressing GFP-mhcA (myosin II heavy chain) | axeA2, axeB2, axeC2, aarA-[aarA-KO], bsR, GFP-mhcA, hygR | |
| abcA10- | abcA10 insertional mutant expressed in AX4 cells | axeA1, axeB1, axeC1, abcA10-, bsR | |
| abcA11- | abcA11 insertional mutant expressed in AX4 cells | axeA1, axeB1, axeC1, abcA11-, bsR | |
| abcA3- | abcA3 insertional mutant expressed in AX4 cells | axeA1, axeB1, axeC1, abcA3-, bsR | |
| abcA4- | abcA4 insertional mutant expressed in AX4 cells | axeA1, axeB1, axeC1, abcA4-, bsR | |
| abcA5- | abcA5 insertional mutant expressed in AX4 cells | axeA1, axeB1, axeC1, abcA5-, bsR | |
| abcA6- | abcA6 insertional mutant expressed in AX4 cells | axeA1, axeB1, axeC1, abcA6-, bsR | |
| abcA7- | abcA7 insertional mutant expressed in AX4 cells | axeA1, axeB1, axeC1, abcA7-, bsR | |
| abcA9- | abcA9 insertional mutant expressed in AX4 cells | axeA1, axeB1, axeC1, abcA9-, bsR | |
| abcB1- | abcB1 insertional mutant expressed in AX4 cells | axeA1, axeB1, axeC1, abcB1-, bsR | |
| abcB2- | abcB2 REMI mutant; the insertion site is 37 bp upstream of the 3'''' boundary of the first exon; note, the insertion appears to have deleted a short stretch of genomic DNA;
get raw sequence data | axeA1, axeB1, axeC1, abcB2-, bsR | |
| abcB4- | abcB4 insertional mutant expressed in AX4 cells | axeA1, axeB1, axeC1, abcB4-, bsR | |
| abcB5- | abcB5 insertional mutant expressed in AX4 cells | axeA1, axeB1, axeC1, abcB5-, bsR | |
| abcC12- | abcC12 insertional mutant expressed in AX4 cells | axeA1, axeB1, axeC1, abcC12-, bsR | |
| abcC13- | abcC13 insertional mutant expressed in AX4 cells | axeA1, axeB1, axeC1, abcC13-, bsR | |
| abcC14- | abcC14 insertional mutant expressed in AX4 cells | axeA1, axeB1, axeC1, abcC14-, bsR | |
| abcC2- | abcC2 insertional mutant expressed in AX4 cells | axeA1, axeB1, axeC1, abcC2-, bsR | |
| abcC3- | abcC3 insertional mutant expressed in AX4 cells | axeA1, axeB1, axeC1, abcC3-, bsR | |
| abcC6- | abcC6 insertional mutant expressed in AX4 cells | axeA1, axeB1, axeC1, abcC6-, bsR | |
| abcC8- | abcC8 insertional mutant expressed in AX4 cells | axeA1, axeB1, axeC1, abcC8-, bsR | |
| abcC9- | Cheater mutant. Remi insertion in abcC9 (ABC transporter C family protein). | axeA1, axeB1, axeC1, abcC9-[pBSR1], bsR | axe, bsR |
| abcD2- | abcD2 insertional mutant expressed in AX4 cells | axeA1, axeB1, axeC1, abcD2-, bsR | |
| abcF1- | abcF1 insertional mutant expressed in AX4 cells | axeA1, axeB1, axeC1, abcF1-, bsR | |
| abcF2- | abcF2 insertional mutant expressed in AX4 cells | axeA1, axeB1, axeC1, abcF2-, bsR | |
| abcF4- | abcF4 insertional mutant expressed in AX4 cells | axeA1, axeB1, axeC1, abcF4-, bsR | |
| abcG10- | abcG10 insertional mutant expressed in AX4 cells | axeA1, axeB1, axeC1, abcG10-, bsR | |
| abcG15- | abcG15 insertional mutant expressed in AX4 cells | axeA1, axeB1, axeC1, abcG15-, bsR | |
| abcG16- | abcG16 insertional mutant expressed in AX4 cells | axeA1, axeB1, axeC1, abcG16-, bsR | |
| abcG17- | abcG17 insertional mutant expressed in AX4 cells | axeA1, axeB1, axeC1, abcG17-, bsR | |
| abcG18- | abcG18 insertional mutant expressed in AX4 cells | axeA1, axeB1, axeC1, abcG18-, bsR | |
| abcG19- | abcG19 insertional mutant expressed in AX4 cells | axeA1, axeB1, axeC1, abcG19-, bsR | |
| abcG2- | abcG2 insertional mutant expressed in AX4 cells | axeA1, axeB1, axeC1, abcG2-, bsR | |
| abcG2-/abcG18- | mdrA1/mdrA2 double null mutant (originally isolated as a suppressor of rtoA-).
mdrA1=mdrA=abcG2;
mdrA2=mdrB=abcG18 | axeA1, axeB1, axeC1, abcG2-/abcG18--[pUCBsrΔBam-mdr], bsR | axe, bsR, no growth in shaking culture |
| abcG2-/abcG18- | mdrA1/mdrA2 double null mutant (originally isolated as a suppressor of rtoA-).
mdrA1=abcG2;
mdrA2=abcG18 | axeA1, axeB1, axeC1, pyr5-6-[pRG24], ura-, abcG2-/abcG18--[pUCBsrΔBam-mdr], bsR | axe, bsR, ura- |
| abcG2-/abcG18-/rtoA- | abcG2-/abcG18-/rtoA- triple null mutant; recapitulation of the rtoA suppressor | axeA1, axeB1, axeC1, pyr5-6-[pRG24], rtoA-[pRtoA-KO ], ura+, mdrA-[pMDR], bsR | axe, bsR |
| abcG22- | abcG22 insertional mutant expressed in AX4 cells | axeA1, axeB1, axeC1, abcG22-, bsR | |
| abcG24- | abcG24 insertional mutant expressed in AX4 cells | axeA1, axeB1, axeC1, abcG24-, bsR | |
| abcG5- | abcG5 insertional mutant expressed in AX4 cells | axeA1, axeB1, axeC1, abcG5-, bsR | |
| abcG6- | abcG6 insertional mutant expressed in AX4 cells | axeA1, axeB1, axeC1, abcG6-, bsR | |
| abcG6- | RAM: regulator of adhesion and motility mutants; abcG6- REMI mutant | axeA2, axeB2, axeC2, abcG6-, [bsRcas], bsR | |
| abcG7- | abcG7 insertional mutant expressed in AX4 cells | axeA1, axeB1, axeC1, abcG7-, bsR | |
| abcH2- | abcH2 insertional mutant expressed in AX4 cells | axeA1, axeB1, axeC1, abcH2-, bsR | |
| abiA- | abiA null mutant in NC4A2 | axe, matA1, abiA-, bsR | |
| abiA- | abiA null mutant in AX3 | axeA1, axeB1, axeC1, abiA-, bsR | |
| abiA-/abiA(1-207) | abiA null strain expressing the full length abiA with the C-terminus removed | axeA1, axeB1, axeC1, abiA-, abiA(1-207), bsR, neoR | |
| abiA-/abiA(45-136) | abiA null strain expressing the full length abiA with both the N- and C-terminus removed | axeA1, axeB1, axeC1, abiA-, abiA(45-136), bsR, neoR | |
| abiA-/abiA(45-332) | abiA null strain expressing the full length abiA with the N-terminus removed | axeA1, axeB1, axeC1, abiA-, abiA(45-332), bsR, neoR | |
| abiA-/AX2 | abiA null mutant in AX2 | axeA2, axeB2, axeC2, abiA-, [bsR-loxP], bsR | |
| abiA-/pirA-/AX2 | abiA/pirA double null mutant in AX2 | axeA2, axeB2, axeC2, abiA-, [loxP], pirA-, [bsR-loxP], bsR | |
| abiA-/scrA- | abiA/scrA double null mutant in NC4A2 | axe, matA1, scrA-[scrA::pyr5-6], abiA-, bsR | |
| abiA-/[act15]:abiA(S166A/S168A/S169A) | unphoshorylatable AbiA expressed in abiA null mutant | axe, matA1, abiA-, [bsRcas], abiA(S166A/S168A/S169A), [pDM304], bsR, neoR | |
| abiA-/[act15]:abiA(S166D/S168D/S169D) | phosphomimetic AbiA expressed in abiA null | axe, matA1, abiA-, [bsRcas], abiA(S166D/S168D/S169D), [pDM304], bsR, neoR | |
| abiA-/[act15]:myc:abiA | abiA null mutant overexpressing myc tagged abiA under the control of the act15 promoter | axe, matA1, scrA-[scrA::pyr5-6], abiA-, [act15]:myc:abiA, bsR | |
| abkD- | Looser mutant identified in cheater screen. Remi insertion in abkD (putative protein serine/threonine kinase;
ABC1 family protein kinase;
ABC1-B subfamily protein kinase). Contains ABC1 kinase (protein kinase-like) domain; yeast ABC1 essential for the electron transfer in the BC(1) complex; E.coli homolog required for ubiquinone biosynthesis).
| axeA1, axeB1 axeC1, abkD-, [bsRcas], bsR | |
| abnC- | RAM: regulator of adhesion and motility mutants; abnC- REMI mutant | axeA2, axeB2, axeC2, abnC-, [bsRcas], bsR | |
| abpA- | alpha-actinin null mutant | axeA2, axeB2, axeC2, abpA-[p?AA], neoR | axe, neoR |
| abpA- | α-actinin mutant | axeA2, axeB2, axeC2, abpA1000 | axe |
| abpA-/abpC- | abpA-/abpC- (alpha-actinin, ABP120) double null mutant | axeA2, axeB2, axeC2, abpA-, abpC-, neoR, phleoR | |
| abpA-/abpC-/sevA- | abpA-/abpC-/sevA- triple null mutant (alpha-actinin, 120 kDa gelation factor, severin) produced by a combination of chemical mutagenesis, homologous recombination, and parasexual genetics. | axeA2, axeB2, axeC2, gelA1000, abp-tr(3), sevA1000, neoR | axe, neoR |
| abpB- | abpB null mutant; hygromycin resistant | axeA2, axeB2, axeC2, abpB- [pHyg-abpA], hygR | axe,hygR |
| abpB- | abpB null mutant; uracil prototroph | axeA1, axeB1, axeC1, pyr5-6-[pRG24], abpB-, ura+ | |
| abpB-/abpA- | abpB/abpC double null mutant | axeA2, axeB2, axeC2, abpB-, [pHyg-abpB], abpA-, [pDabα1.2], neoR, hygR | |
| abpB-/abpC- | abpB/abpC double null mutant | axeA2, axeB2, axeC2, abpB-, [pHyg-abpB], abpC-, [pDgel1.5], hygR, neoR | |
| abpB-/fimA- | abpB/fimA double null mutant, hygromycin and blasticidin resistant in a AX2 background | axeA2, axeB2, axeC2, abpB-, fimA- [bsR-fimA], hygR, bsR | |
| abpB-/[act15]:abpB(D93A/D95A/D97A) | abpB with (D93A/D95A/D97A) substitutions in EF hand region expressed in abpB null | axeA1, axeB1, axeC1, pyr5-6-[pRG24], abpB-, ura+, abpB(D93A/D95A/D97A), [pBORP], neoR | |
| abpB-/[dscB]:abpB(E60K):GFP | GFP-tagged AbpB with E60K substitution expressed in abpB null cells under control of the dscB promotor | xeA2, axeB2, axeC2, abpB-, [pHyg-abpA], abpB(E60K), [pVEII], neoR, hygR | |
| abpC- | abpC (ABP-120 gelation factor) null mutant | axeA2, axeB2, axeC2, abpA-[pDabgel1.5], neoR | axe, neoR |
| abpC- | abpC (gelA1000) mutant | axeA2, axeB2, axeC2, gelA1000, manA2, tsgK21 | axe,tsg |
| abpC- | 120-kD gelation factor chemical null mutant | axeA2, axeB2, axeC2, gelA1000, abp-tr(3), sevA1000 | axe |
| abpC- | abpC null mutant in AX3 | axeA1, axeB1, axeC1, abpC-, [p120cl], neoR | |
