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Hotz, Manuel, Nelson, WJ W James, (2017) ' Pumilio-dependent localization of mRNAs at the cell front coordinates multiple pathways required for chemotaxis. ' Nat Commun 8 1366
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Abstract:Chemotaxis is a specialized form of directed cell migration important for normal development, wound healing, and cancer metastasis. In the social amoeba Dictyostelium discoideum, four signaling pathways act synergistically to maintain directional cell migration. However, it is unknown how these pathways are coordinated in space and time to achieve persistent chemotaxis. Here, we show that the mRNAs and proteins of these four chemotaxis pathways and actin are preferentially enriched at the cell front during dynamic cell migration, which requires the Pumilio-related RNA-binding protein Puf118. Significantly, disruption of the Pumilio-binding sequence in chemotaxis pathway mRNAs, or mislocalization of Puf118 and its target mRNAs to the cell rear perturbs efficient chemotaxis in shallow cAMP gradients, without affecting the abundance of the mRNAs or encoded proteins. Thus, the polarized localization of Puf118-bound mRNAs coordinates the distribution of different chemotaxis pathway proteins in time and space, leading to cell polarization and persistent chemotaxis.
Status: epublish Type: Journal article Source: PUBMED PubMed ID: 29118357

 
Genes addressed in this paper
DDB_G0276255 DDB_G0279557 DDB_G0288551 act1 act10 act11 act12 act13
Topics in this paper
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Chemotaxis/Motility


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Genetic Interactions







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Protein-Nucleic Acid Interactions X X X






Genes addressed in this paper
act14 act15 act16 act19 act2 act20 act21 act22
Topics in this paper
Protein Physical Properties







Protein Functional Domain







Strains/Constructs







Non-mammalian Gene Related







Cellular Location X X X X X X X X
Synonym







Function/Process







Development/Morphogenesis







Chemotaxis/Motility X X X X X X X X
Genetic Interactions







Regulatory Role







DNA/RNA Sequence Features X X X X X X X X
Mutants/Phenotypes







Regulated By X X X X X X X X
Protein-Nucleic Acid Interactions









Genes addressed in this paper
act3 act4 act5 act6 act7 act8 act9 lst8
Topics in this paper
Protein Physical Properties







Protein Functional Domain







Strains/Constructs







Non-mammalian Gene Related







Cellular Location X X X X X X X X
Synonym







Function/Process







Development/Morphogenesis







Chemotaxis/Motility X X X X X X X X
Genetic Interactions







Regulatory Role







DNA/RNA Sequence Features X X X X X X X X
Mutants/Phenotypes







Regulated By X X X X X X X X
Protein-Nucleic Acid Interactions









Genes addressed in this paper
pikF plaA puf118 pufA rasC sgcA talA
Topics in this paper
Protein Physical Properties

X



Protein Functional Domain

X X


Strains/Constructs

X


X
Non-mammalian Gene Related

X



Cellular Location X X X
X X
Synonym

X



Function/Process

X



Development/Morphogenesis

X



Chemotaxis/Motility X X X

X
Genetic Interactions

X


X
Regulatory Role

X



DNA/RNA Sequence Features X X


X
Mutants/Phenotypes

X


X
Regulated By X X


X
Protein-Nucleic Acid Interactions

X X