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Nichols, JME John M E, Paschke, Peggy, Peak-Chew, Sew, Williams, TD Thomas D, Tweedy, Luke, Skehel, Mark, Stephens, Elaine, Chubb, JR Jonathan R, Kay, RR Robert R, (2018) ' The Atypical MAP Kinase ErkB Transmits Distinct Chemotactic Signals through a Core Signaling Module. ' Dev. Cell

 

Abstract:Signaling from chemoattractant receptors activates the cytoskeleton of crawling cells for chemotaxis. We show using phosphoproteomics that different chemoattractants cause phosphorylation of the same core set of around 80 proteins in Dictyostelium cells. Strikingly, the majority of these are phosphorylated at an [S/T]PR motif by the atypical MAP kinase ErkB. Unlike most chemotactic responses, ErkB phosphorylations are persistent and do not adapt to sustained stimulation with chemoattractant. ErkB integrates dynamic autophosphorylation with chemotactic signaling through G-protein-coupled receptors. Downstream, our phosphoproteomics data define a broad panel of regulators of chemotaxis. Surprisingly, targets are almost exclusively other signaling proteins, rather than cytoskeletal components, revealing ErkB as a regulator of regulators rather than acting directly on the motility machinery. ErkB null cells migrate slowly and orientate poorly over broad dynamic ranges of chemoattractant. Our data indicate a central role for ErkB and its substrates in directing chemotaxis.
Status:	aheadofprint	Type:	Journal article	Source:	PUBMED	PubMed ID:	30612939

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	Genes addressed in this paper
	dhkI-1	dhkI-2	erkA	erkB	gacG	gacH	gpaD	gpbA
Topics in this paper
Strains/Constructs	X	X	X	X
Signal Transduction				X			X	X
Chemotaxis/Motility	X	X	X	X	X	X	X	X
Function/Process	X	X		X	X	X	X	X
Development/Morphogenesis	X	X			X	X
Genome-wide Analysis	X	X		X	X	X
Genetic Interactions			X	X
Regulatory Role				X
Regulated By					X	X
Mutants/Phenotypes	X	X	X	X	X	X	X	X

	Genes addressed in this paper
	piaA	pikA	pikB	pikC	pikF	pikG	pkbA	pkgB
Topics in this paper
Strains/Constructs
Signal Transduction
Chemotaxis/Motility
Function/Process
Development/Morphogenesis
Genome-wide Analysis
Genetic Interactions		X	X	X	X	X	X	X
Regulatory Role
Regulated By
Mutants/Phenotypes	X	X	X	X	X	X	X	X