Cells in Motion

Cells in Motion

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Cytokinesis on a surface 

RacE null mutant divides when attached to a substrate.  From A. Lozanne, U. Texas, Austin. 



Cytokinesis in suspension

RacE null mutant aborts cytokinesis in suspension.
From A. Lozanne, U. Texas, Austin.



Digitized wild-type cell in chemotaxis

Three dimensional direct image reconstruction of a D. discoideum amobae crawling in a gradient of cAMP. The chemoattractant source is left of the field. From D. Wessel and D. Soll, Unv. of Iowa



Digitized clathrin heavy chain null cell in chemotaxis 

Three dimensional direct image reconstruction of a clathrin heavy chain null Dictyostelium cell. Note the aberrant cell shape, lack of polarity and numerous fragmented pseudopods. From D. Wessel and D. Soll, Univ. of Iowa.



Random Motility

D. discoideum cells expressing GFP-tagged to a highly conserved actin binding domain (ABD) crawling on a glass surface. DIC and fluorescence images were collected simultaneously. Images were captured every 10 seconds.
From K.M. Pang, E. Lee, and K.A. Knecht,  University of Connecticut.



Side view of moving cell

Side view of amoebae expressing GFP-ABD plated on a glass surface. DIC and fluorescence images were collected simultaneously every 10 seconds by confocal microscopy. From E. Shelden and D.A. Knecht, University of Connecticut.

 

 

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