Fixation techniques for immunonfluorescence

Fixation techniques for immunonfluorescence

Contributed by Richard Gomer.


For new antibodies I suggest trying a variety of fixation techniques, as some antigens work with formaldehyde, others only work with ethanol fixation, etc. Here is a list of fixatives.

Fixatives (try 10 min and 30 min at room temp unless otherwise indicated)

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