Strain Catalog

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Strain Descriptor Strain Summary Genotype Phenotype
DDB_G0290519-RAM: regulator of adhesion and motility mutants; REMI mutant; DDB_G0290519- REMI mutantaxeA2, axeB2, axeC, DDB_G0290519-, [bsRcas], bsR
DDB_G0290535-DDB_G0290535 REMI mutant; the insertion site is 528 bp upstream of the stop codon; insertion at the upper case DpnII restriction site (GATC) in this sequence: tatcatatgGATCaccaacttt;
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axeA1, axeB1, axeC1, DDB_G0290535-, bsR
DDB_G0290685-Cheater mutant. Remi insertion in DDB_G0290685 (unknown gene product; highly repetitive protein: contains close to 100 repeats of the sequences DGENNQ, DGGENNQ, or very related sequences) axeA1, axeB1, axeC1, DDB_G0290685-, [bsRcas], bsR
DDB_G0290803-DDB_G0290803 REMI mutant; the insertion site is 138 bp downstream of the start codon; insertion at the upper case NlaIII restriction site (CATG) in this sequence: atttgataCATGgtttcacaac;
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axeA1, axeB1, axeC1, DDB_G0290803-, bsR
DDB_G0290969-DDB_G0290969 REMI mutant; the insertion site is 301 bp downstream of the start codon; insertion at the uppercase DpnII restriction site (GATC) in this sequence: aaagttgGATCatcacaatca;
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axeA1, axeB1, axeC1, DDB_G0290969-, bsR
DDB_G0291263-DDB_G0291263 REMI mutant; the insertion site is 45 bp upstream of the stop codon;
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axeA1, axeB1, axeC1, DDB_G0291263-, bsR
DDB_G0291350-DDB_G0291350 REMI mutant; the insertion site is 3 bp upstream of the 5 '''' boundary of the 2nd exon; insertion at the upper case NlaIII restriction site (CATG) in this sequence: gtctaCATGagtggggtt;
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axeA1, axeB1, axeC1, DDB_G0291350-, bsR
DDB_G0291464-DDB_G0291464 REMI mutant; the insertion site is 249 bp downstream of 5'''' boundary of the first intron; note, the insertion appears to have deleted a short stretch of genomic DNA;
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axeA1, axeB1, axeC1, DDB_G0291464-, bsR
DDB_G0291800-DDB_G0291800 REMI mutant; the insertion site is 51 bp upstream of the start codon; insertion at the upper case DpnII restriction site (GATC) in this sequence: tggagaattGATCaaaaaa;
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axeA1, axeB1, axeC1, DDB_G0291800-, bsR
DDB_G0291824-DDB_G0291824 REMI mutant; the insertion site is 420 bp downtream of the start codon; insertion at the upper case DpnII restriction site (GATC) in this sequence: ttccatGATCcaaatatgcaa;
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axeA1, axeB1, axeC1, DDB_G0291824-, bsR
DDB_G0291992-DG1003 REMI mutantaxeA1, axeB1, axeC1, DDB_G0291992-, bsR
DDB_G0292148-Cheater mutant. Remi insertion in DDB_G0292148 (Arf GTPase activating protein).axeA1, axeB1, axeC1, DDB_G0292148-, [bsRcas], bsR
DDB_G0292186-DDB_G0292186 null mutant; NDT80/PhoG like DNA-binding domain-containing protein. Total aa/point of insertion: 1133/794. axeA1, axeB1, axeC1, bsR, DDB_G0292186-axe, bsR
DDB_G0292188-DDB_G0292188 null mutant in AX4axeA1, axeB1, axeC1, DDB_G0292188-, bsRcas, bsR
DDB_G0293074-DDB_G0293074- null mutant expressed in AX2 cellsaxeA2, axeB2, axeC2, DDB_G0293074-, [bsR-loxP], bsR
DDB_G0293098-RAM: regulator of adhesion and motility mutants; DDB_G0293098- REMI mutant axeA2, axeB2, axeC2, DDB_G0293098-, [bsRcas], bsR
DDB_G0293116-DDB_G0293116 null mutant; blasticidin resistantaxeA1, axeB1, axeC1, DDB_G0293116-, bsR
DDB_G0293116-DDB_G0293116 null mutant; blasticidin resistantDDB_G0293116-, bsR
DDB_G0293356-DDB_G0293356 REMI mutant in AX4axeA1, axeB1, axeC1, DDB_G0293356-, [pBsrDBglII], bsR
DDB_G0293662-DDB_G0293662 REMI mutant; the insertion site is 288 bp upstream of the stop codon; insertion at the upper case NlaIII restriction site (CATG) in this sequence: tagtattcatCATGcttggattat;
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axeA1, axeB1, axeC1, DDB_G0293662-, bsR
DDB_G0293762-DDB_G0293762 null mutant; blasticidin resistantaxeA1, axeB1, axeC1, DDB_G0293762-, bsR
DDB_G0293982-DDB_G0293982 REMI mutant; the insertion site is 654 bp downstream of the 5'''''''' end of the third exon; insertion at the upper case NlaIII restriction site (CATG) in this sequence: aatacatCATGtgattgta;
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axeA1, axeB1, axeC1, DDB_G0293982-, bsR
DDB_G0348834/DDB_G0348835-REMIREMI mutant; note, the insertion site is in an intergenic region; 182 bp downstream of DDB_G0348834, and 140 bp upstream of DDB_G0348835; also note that a different clone of this mutant is available (DBS0349686)
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axeA1, axeB1, axeC1, DDB_G0348834, DDB_G0348835-, bsR
DDB_G0348834/DDB_G0348835-REMIREMI mutant; note, the insertion site is in an intergenic region; 182 bp downstream of DDB_G0348834, and 140 bp upstream of DDB_G0348835; also note that a different clone of this mutant is available (DBS0349663)
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axeA1, axeB1, axeC1, DDB_G0348834, DDB_G0348835-, bsR
DDB_G0349497_ps/ifkA-REMIREMI mutant; note, the insertion site is in an intergenic region; 771 bp downstream of stop codon of the ifkA gene and 2992 bp upstream of the 5'' boundary of the pseudogene DDB_G0349497_ps;
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axeA1, axeB1, axeC1, DDB_G0349497_ps-, ifkA, bsR
Del16-11pyrimidine (uracil) auxotrophaxeA2, axeB2, axeC2, pyr5-6-[pDU3B1.D], ura-axe,ura-
detA-detA null mutantAxeA2, axeB2, axeC2, detA-, bsR
detA-/[act6]:culDdetA null mutant transformed with 3XHA-tagged repEAxeA2, axeB2, axeC2, detA-, 3XHA-culD, bsR, neoR
detA-/[act6]:GFP:detAGFP marked detA expressed in the detA null mutantAxeA2, axeB2, axeC2, detA-, GFP-detA, bsR, neoR
detA-/[act6]:GFP:detA/[act6]:3xHA:culDGFP marked detA expressed in the detA null mutant supertransformed with 3XHA-tagged culDAxeA2, axeB2, axeC2, detA-, GFP-detA, 3XHA-culD, bsR, neoR
detA-/[act6]:GFP:detA/[act6]:3xHA:repEGFP marked detA expressed in the detA null mutant supertransformed with 3XHA-tagged repEAxeA2, axeB2, axeC2, detA-, GFP-detA, 3XHA-repE, bsR, neoR
detA-/[act6]:repEdetA null mutant transformed with 3XHA-tagged repEAxeA2, axeB2, axeC2, detA-, 3XHA-repE, bsR, neoR
detA-/[cotB]:GFPdetA null mutant expressing the prespore marker cotB-GFPaxeA2, axeB2, axeC2, detA-, cotB-GFP, bsR, ?
detA-/[ecm0]:lacZdetA null mutant expressing the prestalk marker ecm0-LacZaxeA2, axeB2, axeC2, detA-, ecm0-LacZ, bsR, neoR
detA-/[ecmA0]:GFPdetA null mutant expressing the prestalk marker ecmA0-GFPaxeA2, axeB2, axeC2, detA-, ecmA0-GFP, bsR, ?
detA-/[ecmA0]:lacZdetA null mutant expressing the prestalk marker ecmA0-LacZaxeA2, axeB2, axeC2, detA-, ecmA0-LacZ, bsR, neoR
detA-/[ecmA]:lacZdetA null mutant expressing the prestalk marker ecmA-LacZaxeA2, axeB2, axeC2, detA-, ecmA-LacZ, bsR, neoR
detA-/[ecmB]:lacZdetA null mutant expressing the prestalk marker ecmB-LacZaxeA2, axeB2, axeC2, detA-, ecmB-LacZ, bsR, neoR
DG1022-DG1022 REMI mutantaxeA1, axeB1, axeC1, DG1022-, bsR
DG1039-DG1039 REMI mutantaxeA1, axeB1, axeC1, DG1039-, bsR
DG1040-DG1040 REMI mutantaxeA1, axeB1, axeC1, DG1040-, bsR
DG1041-DG1041 REMI mutantaxeA1, axeB1, axeC1, DG1041-, bsR
DG1054-DG1054 REMI mutantaxeA1, axeB1, axeC1, DG1054-, bsR
DG1060-DG1060 REMI mutant axeA1, axeB1, axeC1, DG1060-, bsR
DG1062-DG1062 REMI mutantaxeA1, axeB1, axeC1, DG1062-, bsR
DG1074-DG1074 REMI mutantaxeA1, axeB1, axeC1, DG1074-, bsR
DG1080-DG1080 REMI mutantaxeA1, axeB1, axeC1, DG1080-, bsR
DG1091-DG1091 REMI mutantaxeA1, axeB1, axeC1, DG1091-, bsR
DG1098-DG1098 REMI mutantaxeA1, axeB1, axeC1, DG1098-, bsR
DG1104-DG1104 REMI mutantaxeA1, axeB1, axeC1, DG1104-, bsR
DG1104-DG1104 REMI mutant; the insertion site is 1,174 bp upstream of the 3'''' boundary of the third exon; insertion at the upper case DpnII restriction site (GATC) in this sequence: tcacgtatGATCattgaagat;
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axeA1, axeB1, axeC1, DDB_G0283535-, bsR
DG1106-DG1106 REMI mutantaxeA1, axeB1, axeC1, DG1106-, bsR
DG1110-DG1110 REMI mutantaxeA1, axeB1, axeC1, DG1110-, bsR
DG1112-DG1112 REMI mutantaxeA1, axeB1, axeC1, DG1112-, bsR
DG1113-DG1113 REMI mutantaxeA1, axeB1, axeC1, DG1113-, bsR
DG1122-DG1122 REMI mutantaxeA1, axeB1, axeC1, DG1122-, bsR
DG1124-DG1124 REMI mutantaxeA1, axeB1, axeC1, DG1124-, bsR
DG2033-DG2033 REMI mutantaxeA1, axeB1, axeC1, DG2033-, bsR
DG2044-DG2044 REMI mutantaxeA1, axeB1, axeC1, DG2044-, bsR
dgat1-dgat1 null mutant in AX2axeA2, axeB2, axeC2, dgat1-, [bsR-loxP], bsR
dgat1-/dgat2-dgat1/dgat2 double null mutantaxeA2, axeB2, axeC2, dgat1-, [bsR-loxP], dgat2-, [loxP]
dgat1-/dgat2-/[act15]:GFP:dgat2Overexpression of GFP:dgat2 in the dgat1/dgat2 double null mutant axeA2, axeB2, axeC2, dgat1-, [bsR-loxP], dgat2-, [loxP], [act15]:GFP:dgat2, neoR
dgcA-dgcA null mutantaxe, matA1, dgcA-, bsR
dgcA-dgcA deletion mutant using pdgcA_KO_443axeA1, axeB1, axeC1, dgcA-, [pDgcA-KO], bsR
dgcA-/[act15]:dgcA(1-124):YFPC-terminal deletion (125-521) dgcA-YFP expressed in the dgcA null mutantaxe, matA1, dgcA-, [act15]::dgcA(1-124):YFP, bsR, neoR
dgcA-/[act15]:dgcA(1-273):YFPC-terminal deletion (274-521) dgcA-YFP expressed in the dgcA null mutantaxe, matA1, dgcA-, [act15]:dgcA(1-273):YFP, bsR, neoR
dgcA-/[act15]:dgcA(125-521):YFPN-terminal deletion (1-124) dgcA-YFP expressed in the dgcA null mutantaxe, matA1, dgcA-, [act15]:dgcA(125-521):YFP, bsR, neoR
dgcA-/[act15]:dgcA(274-521):YFPN-terminal deletion (1-273) dgcA-YFP expressed in the dgcA null mutantaxe, matA1, dgcA-, [act15]:dgcA(274-521):YFP, bsR, neoR
dgcA-/[act15]:dgcA(350-521):YFPN-terminal deletion (1-349) dgcA-YFP expressed in the dgcA null mutantaxe, matA1, dgcA-, [act15]:dgcA(350-521):YFP, bsR, neoR
dgcA-/[act15]:dgcA-Ppal:YFPP. pallidum dgcA-YFP expressed in the D. discoideum dgcA null mutantaxe, matA1, dgcA-, [act15]:dgcA-Ppal:YFP, bsR, neoR
dgcA-/[act15]:dgcA:YFPdgcA-YFP expressed in the dgcA null mutantaxe, matA1, dgcA-, [act15]:dgcA:YFP, bsR, neoR
dgkA-dgkA null mutant; thymidine prototrophaxeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, dgkA-[pThy1-dgkA] thy+
dgkA-dgkA null mutant in AX2axeA2, axeB2, axeC2, [dgkA-], [bsR-loxP], bsR
DH1Margaret Clarke lab strain; axenic; uracil auxotrophaxeA1, axeB1, axeC1, pyr5-6-[pRG24], ura-axe,ura-
DH1Peter Devreotes original lab strain; axenic; uracil auxotrophaxeA1, axeB1, axeC1, pyr5-6-[pRG24], ura-axe,ura-,wt
DH1generic axenic, uracil auxotroph strain, used for curation of parental strains when the specific DH1 is not availableaxeA1, axeB1, axeC1, pyr5-6- [pRG24], ura-
DH1DH1 from the Golstein lab.axeA1, axeB1, axeC1, pyr5-6-[pRG24], ura-axe, ura-, wt
DH1Terry OHalloran lab strain.axeA1, axeB1, axeC1, pyr5-6-[pRG24], ura-axe, ura-
DH1Doug Robinson lab strain; axenic; uracil auxotrophaxeA1, axeB1, axeC1, pyr5-6-[pRG24], ura-axe,ura-,wt
DH1Cathy Chia lab strain; axenic; uracil auxotrophaxeA1, axeB1, axeC1, pyr5-6-[pRG24], ura-aux,axe,wt
DH1Rob Insall lab strain; axenic; uracil auxotrophaxeA1, axeB1, axeC1, pyr5-6-[pRG24], ura-axe,ura-
DH1Richard Kessin lab strain; axenic; uracil auxotrophaxeA1, axeB1, axeC1, pyr5-6-[pRG24], ura-axe,ura-,wt
DH1-10DH1-10 from the Cosson lab.axeA1, axeB1, axeC1, pyr5-6- [pRG24], ura-axe, ura-
DH10Steve Alexander DH1 lab strain; axenic; uracil auxotrophaxeA1, axeB1, axeC1, pyr5-6- [pRG24], ura-
dhkA-dhkA null mutant (isolated as fbxA suppressor)axeA1, axeB1, axeC1, pyr5-6-[pRG24], ura-, dhkA-[dhkA-KO], bsRaxe,ura-,bsR,few spores
dhkA-dhkA null mutantaxeA1, axeB1, axeC1, dhkA-axe,stalky
dhkA-dhkA REMI mutantaxeA1, axeB1, axeC1, dhkA-[DIV10], ura+axe,stalky
dhkA-/dhkB-dhkA/dhkB double null mutantaxeA1, axeB1, axeC1, dhkA-[DIV10], ura+, dhkB-[p2C3/BSR], bsRaxe,bsR,stalky (no spores)
dhkA-/fbxA-dhkA/fbxA double null mutant; fbxA suppressor (dhkA null); same as HR49axeA1, axeB1, axeC1, pyr5-6-[pRG24], fbxA-[pJB1], ura+, dhkA-[pBSR1], bsRaxe,bsR
dhkA-/fbxA-dhkA/fbxA double null mutant; suppressor of fbxA- (dhkA null); same as HR50axeA1, axeB1, axeC1, pyr5-6-[pRG24], fbxA-[pJB1], ura+, dhkA-[pBSR1], bsRaxe,bsR
dhkA-/fbxA-dhkA/fbxA double null mutant; recapitulation of fbxA suppression by dhkAaxeA1, axeB1, axeC1, pyr5-6-[pRG24], fbxA-[pJB1], ura+, dhkA-[dhkA-KO], bsRaxe,bsR
dhkA-/pkaR-dhkA/pkaR double null mutantaxeA1, axeB1, axeC1, dhkA-[DIV10], ura+, pkaR-, [bsRcas], bsR
dhkA-/regA-dhkA/regA double null mutantaxeA1, axeB1, axeC1, dhkA-[DIV10], ura+, regA-, [bsRcas], bsR
dhkA-/[act15]:dhkA(D2076N)DhkA with D2076N substitution expressed in dhkA nullaxeA1, axeB1, axeC1, dhkA-[DIV10], ura+, dhkA(D2076N), [pDdgal15], neoR
dhkA-/[act15]:dhkA(H1396Q)DhkA with H1396Q substitution expressed in dhkA null cellsaxeA1, axeB1, axeC1, dhkA-[DIV10], ura+, dhkA(H1396Q), [pDdgal15], neoR
dhkA-/[act15]:dhkA(H2396Q)/[act15]:dhkA(D2076N)DhkA with H1396Q and D2076N substitutions expressed in dhkA null cellsaxeA1, axeB1, axeC1, dhkA-[DIV10], ura+, dhkA(H1396Q), [pDdgal15], dhkA(D2076N), [pDdgal15], neoR
dhkA-/[pkaC]:pkaCpkaC overexpression (KP) in dhkA null cells, neomycin resistant, AX4 derivativeaxeA1, axeB1, axeC1, dhkA-[DIV10], ura+, pkaC [K-Neo], neoR
dhkB-dhkB null mutantaxeA1, axeB1, axeC1, dhkB-, bsR
dhkB-/[pkaC]:pkaCdhkB null mutant overexpressing pkaC under the control of its own promoteraxeA1, axeB1, axeC1, dhkB-, [pkaC]:pkaC, bsR , neoR
dhkC-dhkC null mutantaxeA1, axeB1, axeC1, dhkC-[dhkC-bsr], bsR
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