| Strain Descriptor |
Strain Summary |
Genotype |
Phenotype |
| QS31|tgrB1/[act15]:RFP/[cotB]:GFP | AX4 cells with an additional tgrB1 allele from QS31 strain; coexpresses tdTomato under act15 promote and superfolder GFP under cotB promoter | axeA1, axeB1, axeC1, QS31/trgrB1, RFP, [act15:tdTomato], GFP, [cotB:sfGFP], bsR, hygR, neoR | |
| QS4/[act15]:GFP | QS4, natural isolate expressing GFP under Act15 promoter | QS4, GFP, neoR | |
| QS4|tgrB1/tgrC1/[act15]:RFP | merodiploid strain expressing tgrB1 and tgrC1 alleles of AX4 in the native locus and expressing an extra copy of both tgrB1 and tgrC1 alleles from QS4 inserted randomly in the genome and under the QS4 promoter; expressing RFP(tdTomato) under the act15 promoter | axeA1, axeB1, axeC1, QS4/tgrB1-tgrC1, RFP, [act15:tdTomato], bsR, neoR | |
| qtrt1- | qtrt1 null mutant in AX2-214 | axeA2, axeB2, axeC2, qtrt1-, [bsRcas], bsR | |
| r21- | knockout of the r21 class Ia RNA | axeA1, axeB1, axeC1, r21-, [bsRcas], bsR | |
| r33- | knockout of the r33 class Ia RNA | axeA1, axeB1, axeC1, r33-, [bsRcas], bsR | |
| R7 | ubiquitinylation pathway | axeA1, axeB1, axeC1, pyr5-6-[pGP24], nosA-[pJB-1], ura+ | |
| rab7A- | rab7A null mutant expressed in AX2 cells | axeA2, axeB2, axeC2, rab7A-, [bsRcas], bsR | |
| rabO- | rabO REMI mutant; the insertion site is 4 bp into the first intron; insertion at the upper case DpnII restriction site (GATC) in this sequence: agggtatGATCcaattcta;
get raw sequence data | axeA1, axeB1, axeC1, rabO-, bsR | |
| racB- | racB null mutant; blasticidin resistant | axeA1, axeB1, axeC1 racB-[bsRcas], bsR | |
| racC- | racC null | axeA1, axeB1, axeC1, racC-, bsR | |
| racC- | racC null expressed in AX2 cells | axeA2, axeB2, axeC2, racC-, [bsR-loxP], bsR | |
| racC-/[act15]:CFP:racC | racC null mutant expressing racC under the act15 promoter, fused with cyan fluorescence protein | axeA1, axeB1, axeC1, [act15]:racC-/CFP:racC, neoR, bsR | |
| racE- | RacE-null mutant (Rho GTPase) | axeA1, axeB1, axeC1, pyr5-6-[pRG24], ura+, racE-[pRHI30] | axe, |
| racE- | racE null cells expressed in AX2 cells | axeA2, axeB2, axeC2, racE-, [bsR-loxP], bsR | |
| racE- | racE null mutant in AX2 | axeA2, axeB2, axeC2, racE-, [bsR-loxP], bsR | |
| racE-/DH1 | Deletion mutant of RacE | axeA1, axeB1, axeC1, racE-[?] | axe |
| racE-/[act15]:fttB | fttB overexpressed in the racE null mutant | axeA1, axeB1, axeC1, pyr5-6-[pRG24], ura+, racE-[pRHI30], fttB, neoR | |
| racE-/[act15]:fttB:GFP | fttB-GFP overexpressed in the racE null mutant | axeA1, axeB1, axeC1, pyr5-6-[pRG24], ura+, racE-[pRHI30], fttB:GFP, neoR | |
| racE-/[act15]:GFP:racE(G20V) | constitutively GTP-bound GFP-tagged racE with G20V substitution expressed in racE null
| axeA2, axeB2, axeC2, racE-, [bsR-loxP], racE(G20V), [pTX-GFP], bsR, neoR | |
| racE-/[act15]:GFP:racE(G23V) | GFP-tagged racE with G23V expressed in racE null | axeA2, axeB2, axeC2, racE-, [bsR-loxP], racE(G23V), [pTX-GFP], bsR, neoR | |
| racE-/[act15]:GFP:racE(S192D) | phospho-mimetic, GFP-tagged racE(S192D) expressed in racE null | axeA2, axeB2, axeC2, racE-, [bsR-loxP], racE(S192D), [pTX-GFP], bsR, neoR | |
| racE-/[act15]:GFP:racE(T25N) | constitutively GDP-bound, GFP-tagged racE(T25N) expressed in racE null | axeA2, axeB2, axeC2, racE-, [bsR-loxP], racE(T25N), [pTX-GFP], bsR, neoR | |
| racE-/[act15]:GFP:racE(T25N/S192D) | constitutively GDP-bound, phospho-mimetic, GFP-tagged racE(T25N/S192D) expressed in racE null | axeA2, axeB2, axeC2, racE-, [bsR-loxP], racE(T25N/S192D), [pTX-GFP], bsR, neoR | |
| racE-/[act15]:GFP:rasC(Q62L) | constitutively GTP-bound, GFP-tagged rasC with Q62L substitution expressed in racE null | axeA2, axeB2, axeC2, racE-, [bsR-loxP], rasC(Q62L), [pTX-GFP], bsR, neoR | |
| racE-/[act15]racE(G20V) | constitutively active racE with G20V substitution expressed in racE null mutant | axeA1, axeB1, axeC1, pyr5-6-[pRG24], ura+, racE-[pRHI30], racE(G20V), pTIKL, neoR | |
| racE-/[act15]racE(T25N) | inactive racE expressed in racE null mutant | axeA1, axeB1, axeC1, pyr5-6-[pRG24], ura+, racE-, [pRHI30], racE(T25N), pTIKL, neoR | |
| racF1- | racF1 null mutant in AX2 | axeA2, axeB2, axeC2, racF1-, [bsRcas], bsR | |
| racF2(G12V) | Overexpression of constitutively active GFP-RacF2 under the act15 promoter (V12 background) | matA2, racF2-[pGEG02-V], bsR, [pRacF2-G12V], neoR | bsR,neoR,aggregation defective |
| racF2(N17T) | Overexpression of dominant negative GFP-racF2 under the act15 promoter (V12 background). | matA2, racF2-[pGEG02-V], bsR, [pRacf2-N17T], neoR | bsR,neoR,increased cell adhesion |
| racF2- | RacF2-null mutant in V12 | matA2, racF2-[pGEG02-V], bsR | bsR,reduced cell adhesion |
| racF2-/[act15]:GFP:racF2 | Overexpression of GFP:RacF2 in the V12 racF2-null mutant under the actin15 promoter (recapitulation) | matA2, racF2-[pGEG02-V], bsR, [pRacF2-WT], neoR | bsR,neoR |
| racG- | racG null in AX2 | axeA2, axeB2, axeC2, racG-, bsR | |
| racH- | racH null mutant | axeA2, axeB2, axeC2, racH::BSR | |
| rapC- | rapC null mutant in KAX3 | axeA1, axeB1, axeC1, rapC-, [bsRcas], bsR | |
| rapC-/[act15]:GFP:rapA | rapA expressed in rapC null mutant | axeA1, axeB1, axeC1, rapC-, [bsRcas], rapA, [pEXP4(+)], bsR, neoR | |
| rapC-/[act15]:GFP:rapA/[act15]:GFP:rapC(188-278) | RapA fused with the C-terminal residues of RapC expressed in rapC null mutant | axeA1, axeB1, axeC1, rapC-, [bsRcas], rapA, [pEXP4(+)], rapC(188-278), pEXP4(+), bsR, neoR | |
| rapC-/[act15]:GFP:rapC(1-205) | rapC lacking the tail region (rapC(1-205) expressed in rapC null mutant | axeA1, axeB1, axeC1, rapC-, [bsRcas], rapC(1-205), bsR, neoR | |
| rapgap1- | rapgap1 null mutant | axeA1, axeB1, axeC1, rapgap1-, bsR | |
| rapgap1-/[act15]:GFP:rapgap1 | rapgap1 null mutant overexpressing GFP-rapGAP1 | axeA1, axeB1, axeC1, rapgap1-, bsR, neoR, [act15/GFP:rapgap1] | |
| rapgap3- | rapgap3 null mutant in AX2 | axeA2, axeB2, axeC2, rapgap3-, [bsRcas], bsR | |
| rapgap3-/AX2 | rapgap3 null mutant in AX2 | axeA2, axeB2, axeC2, rapgap3-, [bsRcas], bsR | |
| rapgap3-/KAX3 | rapgap3 null mutant in KAX3 | axeA1, axeB1, axeC1, rapgap3-, [bsRcas], bsR | |
| rapgap9- | rapgap9 null mutant expressed in KAX3 cells | axeA1, axeB1, axeC1, rapgap9-, [bsRcas], bsR | |
| rapgap9-/[act15]:GFP:rapgap9 | RapGAP9 under control of the actin 15 promotor expressed in rapgap9 null cells | axeA1, axeB1, axeC1, rapgap9-, [bsRcas], GFP:rapgap9, bsR, neoR | |
| rapgapB- | rapgapB null in AX4 cells | axeA1, axeB1, axeC1, rapgapB-, [bsRcas], bsR | |
| rapgapB-/[ecmAO]:RFP | rapgapB null expressing RFP under control of prestalk promotor ecmAO | axeA1, axeB1, axeC1, rapgapB-, [bsRcas], RFP, [ecmAO-RFP], bsR, neoR | |
| rapgapB-/[ecmA]:lacZ | rapgapB null expressing LacZ under control of prestalk ecmA promotor | axeA1, axeB1, axeC1, rapgapB-, [bsRcas], lacZ, [pEcmA-Gal], bsR, neoR | |
| rapgapB-/[ecmO]:lacZ | rapgapB null expressing LacZ under control of prestalk ecmO promotor | axeA1, axeB1, axeC1, rapgapB-, [bsRcas], lacZ, [pEcmO-Gal], bsR, neoR | |
| rapgapB-/[pspA]:RFP | rapgapB null expressing RFP under control of prespore promotor pspA | axeA1, axeB1, axeC1, rapgapB-, [bsRcas], RFP, [pspA:RFP], bsR, neoR | |
| rasB- | rasB null; strain is unstable and reverts | axeA2, axeB2, axeC2, rasB-, bsR | |
| rasB- | rasB null in AX2-214 | axeA2, axeB2, axeC2, rasB-, [bsR-loxP], bsR | |
| rasC- | rasC null mutant in AX2 | axeA2, axeB2, axeC2, rasC-, [bsRcas], bsR | |
| rasC-/AX2 | rasC null in AX2 | axeA2, axeB2, axeC2, rasC-[rasC::bsr], bsR | axe, bsR, agg- |
| rasC-/dmpA- | rasC-/dmpA- double null mutant
(REMI of rasC-, disrupting the dmpA gene) (suppressor analysis) | axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, rasC-[rasC::thy1], thy+, dmpA-[pBSR1], bsR | axe, bsR |
| rasC-/JH10 | rasC null in JH10 | axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, rasC-[rasC::thy1], thy+ | axe,thy+,ura+,agg- |
| rasC-/pikD1 | REMI of rasC-, disrupting the pikD gene, but there is still a pikD transcript. | axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, rasC-[rasC::thy1], thy+, pikD1[pBSR1], bsR | axe, bsR |
| rasC-/rasD- | rasC/rasD double null mutant | axeA2, axeB2, axeC2, rasC-[rasC::bsr], rasD-, bsR | |
| rasC-/rasG- | rasC-/rasG- double null mutant in JH10 | axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, rasG-[rasG::thy1], thy+, rasC-[rasC::bsr], bsR | axe, bsR |
| rasC-/rasG-/[act15]:carA | rasC-/rasG- double null mutant overexpressing carA from the act15 promoter. | axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, rasG-[rasG::thy1], thy+, rasC-[rasC::bsr], bsR, [pCAR1-B18], neoR | axe, bsR, neoR |
| rasC-/rasG-/[rasG]:rasD | RasD under control of the rasG promotor expressed in rasC/rasG double null cells | axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, rasG-[rasG::thy1], thy+, rasC-[rasC::bsr], rasD, [pGEM-T-Easy], bsR, neoR | |
| rasC-/rasG-/[rasG]:rasD(1-104):rasG(103-189) | RasD N-terminal RasG C-terminal chimera under control of the rasG promotor expressed in rasC/rasG double null cells | axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, rasG-[rasG::thy1], thy+, rasC-[rasC::bsr], rasD(1-104):rasG(103-189), [pGEM-T-Easy], bsR, neoR | |
| rasC-/rasG-/[rasG]:rasG | RasG under control of the rasG promotor expressed in rasC/rasG double null cells | axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, rasG-[rasG::thy1], thy+, rasC-[rasC::bsr], rasG, [pGEM-T-EASY], bsR, neoR | |
| rasC-/rasG-/[rasG]:rasG(1-104):rasD(103-187) | RasG N-terminal/RasD C-terminal chimera under control of the rasG promotor expressed in rasC/rasG double null cells | axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, rasG-[rasG::thy1], thy+, rasC-[rasC::bsr], rasG(1-104):rasD(103-187), [pGEM-T-Easy], bsR, neoR | |
| rasC-/rasG-/[unk]:carA | CarA expressed in rasC-/rasG- double mutant | axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, rasG-[rasG::thy1], thy+, rasC-[rasC::bsr], carA, bsR | |
| rasC-/rasS- | rasC/rasS double null mutant in AX2 | axeA2, axeB2, axeC2, rasC-, [bsRcas], rasS-[hygRcas], bsR, hygR | |
| rasC-/[act15]:gbpD | gbpD under the control of the act15 promoter expressed in a rasC null mutant; neomycin resistant | axeA1, axeB1, axeC1, rasC-, [act15/gbpD], neoR | |
| rasC-/[rasC]:rasG | RasG under control of rasC promotor expressed in rasC null | axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, rasC-[rasC::thy1], thy+, rasG, [pGEM], neoR | |
| rasC-/[rasC]:rasG(1-77(D30A)):rasC(79-189) | chimeric RasG/RasG protein under control of rasC promotor expressed in rasC null | axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, rasC-[rasC::thy1], thy+, rasG(1-77(D30A)):rasC(79-189), [pGEM], neoR | |
| rasC-/[rasC]:rasG(D30A) | RasG with D30A substitution under control of rasC promotor expressed in rasC null | axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, rasC-[rasC::thy1], thy+, rasG(D30A), [pGEM], neoR | |
| rasC-[act15]:rasG(S17N) | doxycycline-inducible, dominant negative RasG under the control of the act15 promoter in rasC null cells | axeA2, axeB2, axeC2, rasC-, [bsRcas], rasG(S17N), [pDM310], bsR, neoR | |
| rasD- | rasD null mutant in AX2 | axeA2, axeB2, axeC2, rasD-[pATW5], bsR | axe,bsR |
| rasD-/AX3 | rasD null mutant in AX3 | axeA1, axeB1, axeC1, rasD-, [bsRcas], bsR | |
| rasG-/AX2 | rasG null in AX2 | axeA2, axeB2, axeC2, rasG::bsR | |
| rasG-/AX2 | rasG- in AX2 | axeA2, axeB2, axeC2, rasG::bsR | |
| rasG-/AX3 | rasG null in AX3 | axeA1, axeB1, axeC1, rasG::bsR | |
| rasG-/AX3 | rasG null mutant in AX3 | axeA1, axeB1, axeC1, rasG-, [bsRcas], bsR | |
| rasG-/JH10 | rasG- in JH10 | axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, thy-rasG::thy1, thy+ | axe, bsR |
| rasG-/rasS- | rasG/rasS double null mutant in AX2 | axeA2, axeB2, axeC2, rasG-, [bsRcas], rasS-, [hygRcas], bsR, hygR | |
| rasG-/[act15]:FLAG:rasG(K169R | FLAG tagged rasG with point mutatations eliminating 4 RasG ubiquitination sites in rasG null cells | axeA2, axeB2, axeC2, rasG-, FLAG:rasG(K169R, K173R, K176R, K178R), [pEXP4(+)], bsR, neoR | |
| rasG-/[act15]:FLAG:rasG(K169R | FLAG tagged rasG with point mutatations eliminating 7 RasG ubiquitination sites in rasG null cells | axeA2, axeB2, axeC2, rasG-, FLAG:rasG(K169R, K173R, K176R, K178R, K179R, K180R, K184R), [pEXP4(+)], bsR, neoR | |
| rasG-/[dscB]:rasG(S17N) | dominant-negative RasG expressed in rasG null cells | axeA2, axeB2, axeC2, rasG-, [bsRcas], rasG(S17N), bsR, neoR | |
| rasG-/[OE]:rasB | rasG null overexpressing rasB | axeA2, axeB2, axeC2, rasG-, unk:rasB, bsR, neoR | |
| rasG-/[rasG]:rasD | rasG null overexpressing rasD | axeA2, axeB2, axeC2, rasG-, rasG:rasD, bsR, neoR | |
| rasG-gly12 | Wild-type rasG-gly12 transformant | axeA2, axeB2, axeC2, [rasG-gly12], neoR | axe,neoR |
| rasS-/AX2 | rasS- in AX2 | axeA2, axeB2, axeC2, rasS::bsR | |
| rasS-/AX2 | rasS null mutant in AX2 | axeA2, axeB2, axeC2, rasS-, [hygRcas], hygR | |
| rasS-/DdB | rasS null mutant in non-axenic DbB | wt, rasS-, [pDM1081], hygR | |
| rasS-/DH1 | rasS null in DH1 | axeA1, axeB1, axeC1, rasS- | |
| rasZ- | rasZ null mutant; blasticidin resistant | axeA1, axeB1, axeC1, rasZ-, bsR | |
| rbdA- | rbdA null mutant in AX2-214 | axeA2, axeB2, axeC2, rbdA-, [bsRcas], bsR | |
| rbdB- | rbdB null mutant in AX2 | axeA2, axeB2, axeC2, rbdB-, [bsRcas], bsR | |
| rbg3- | rbg3 null mutant expressed in AX2 cells | axeA2, axeB2, axeC2, rbg3-, [bsRcas], bsR | |
| rblA- | rblA null mutant | rblA-, axeA1, axeB1, axeC1, bsrR | rapid development |
| rblA-/[act15]:GFP | rblA null mutant expressing GFP under the control of the actin 15 promoter | axeA2, axeB2, axeC2, rblA-, [act15]:GFP, bsR? | |
| rblA-/[act15]:GFP/[act15]:myc:btg | GFP-labelled rblA- cells over-expressing myc-tagged Btg under control of the act15 promotor. | axeA2, axeB2, axeC2, rblA-, [act15]:GFP, [act15]:myc:btg, neoR, bsR? | |
| rblA-/[act15]:RFP/[act15]:myc:btg | GFP-labelled rblA- cells over-expressing myc-tagged Btg under control of the act15 promotor. | axeA2, axeB2, axeC2, rblA-, [act15]:RFP, [act15]:myc:btg, neoR, bsR? | |
| rblA-/[btg]:lacZ | rblA- null cells expressing labile version of lacZ under the control of the btg promoter | axeA2, axeB2, axeC2, rblA-, [btg]:lacZ, bsr, neoR | |
| rbrA- | rbrA null mutant; uracil prototroph | axeA1, axeB1, axeC1, pyr5-6+, rbrA- | |
| rbrA-/[unk]:rbrA | rbrA null mutant rescued by expression of rbrA | axeA1, axeB1, axeC1, rbrA-, [rbrA], bsR | |
