| Strain Descriptor |
Strain Summary |
Genotype |
Phenotype |
| abpC-/[act15]:abpC | abpC (filamin, 120-kD gelation factor) overexpressor in abpC null mutant; derived from AX2-214, neomycin resistant | axeA2, axeB2, axeC2, gelA1000 [pPROF389] neoR | |
| abpC-/[act15]:abpC | abpC overexpressed in abpC null mutant | axeA1, axeB1, axeC1, abpC-, [p120cl], [g7PYR5Δ6.2], ura-, abpC, [pABP-120PYR], neoR | |
| abpC-/[act15]:abpC(1-348:449-857):GFP | C-terminal fusion of GFP abpC missing rod domain 2 expressed in an abpC null mutant | axeA2, axeB2, axeC2, abpC-, abpC(1-348/449-857):GFP, bsR | |
| abpC-/[act15]:abpC(1-448):GFP | C-terminal fusion of GFP abpC missing rod domains 3-6 expressed in an abpC null mutant | axeA2, axeB2, axeC2, abpC-, abpC(1-448):GFP, bsR | |
| abpC-/[act15]:abpC(1-556):GFP | C-terminal fusion of GFP abpC missing rod domains 4-6 expressed in an abpC null mutant | axeA2, axeB2, axeC2, abpC-, abpC(1-556):GFP, bsR | |
| abpC-/[act15]:abpC(1-556:644-857):GFP | C-terminal fusion of GFP abpC missing rod domain 4 expressed in an abpC null mutant | axeA2, axeB2, axeC2, abpC-, abpC(1-556/644-857):GFP, bsR | |
| abpC-/[act15]:abpC(1-669):GFP | C-terminal fusion of GFP abpC missing the 5th and 6th rod domain expressed in an abpC null mutant | axeA2, axeB2, axeC2, abpC-, abpC(1-669):GFP, bsR | |
| abpC-/[act15]:abpC(1-739):GFP | C-terminal fusion of GFP abpC missing the 6th rod domain expressed in an abpC null mutant | axeA2, axeB2, axeC2, abpC-, abpC(1-739):GFP, bsR | |
| abpC-/[act15]:abpC(149-857):GFP | C-terminal fusion of GFP abpC missing the actin binding domain expressed in an abpC null mutant | axeA2, axeB2, axeC2, abpC-, abpC(149-857):GFP, bsR | |
| abpC-/[act15]:abpC:GFP | C-terminal fusion of GFP to full length abpC (ABP-120, ddFLN) expressed in an abpC null mutant | axeA2, axeB2, axeC2, abpC-, abpC:GFP, neoR | |
| abpC-/[act15]:GFP:abpC(9-148) | N-terminal fusion of GFP to the actin binding domain of abpC (ABP-120, ddFLN) expressed in an abpC null mutant | axeA2, axeB2, axeC2, GFP:abpC(9-148), [pDXA-GABD], neoR | |
| abpC-/[act15]:qkgA(425-1553) | N-terminally truncated QkgA with increased basal activity in abpC null mutant | axeA2, axeB2, axeC2, abpA-[pDabgel1.5], qkgA(425-1553), pEXP(+), neoR | |
| abpC-/[cotB]:abpC:GFP | C-terminal fusion of GFP to abpC under the control of the prespore cotB promoter expressed in an abpC null mutant | axeA2, axeB2, axeC2, abpC-, [cotB]:abpC:GFP, bsR | |
| abpC-/[ecmA]:abpC:GFP | C-terminal fusion of GFP to abpC under the control of the prestalk ecmA promoter expressed in an abpC null mutant | axeA2, axeB2, axeC2, abpC-, [ecmA]:abpC:GFP, bsR | |
| abpD- | abpD null mutant; blasticidin resistant | axeA2, axeB2, axeC2, abpD-[abpD-bsr], bsR | |
| abpE- | actin binding protein (abpE) null mutant | axeA2, axeB2, axeC2, abpE-[abpE-bsr], bsR | axe, bsR, increased lateral pseudopodia |
| abpE- | abpE null mutant in AX2 | axeA2, axeB2, axeC2, abpE-, bsR | |
| abpE-/[act15]:abpE | abpE null mutant overexpressing abpE | axeA2, axeB2, axeC2, abpE-, abpE+, bsR, neoR | |
| abpG- | abpG null mutant in AX2 | axeA2, axeB2, axeC2, abpG-, [bsRcas], bsR | |
| acaA- | ACA-,adenylyl cyclase null (remake of CAP1) | axeA1, axeB1, axeC1, acaA-, [pACA.URA], pyr5-6+, [pRG24] | axe,agg- |
| acaA- | acaA null in AX2 | axeA2, axeB2, axeC2, acaA-[BsRCas], bsR | axe, bsR, agg- |
| acaA- | acaA- mutant in JH10 obtained by targeted disruption of the acaA gene. | axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, acaA-[ACA-thy], thy+ | axe, agg- |
| acaA- | acaA- mutant obtained in screen of AX4 cells mutagenized by remi using pBSR1 and DpnII. | axaA1, axeB1, axeC1, acaA-[pBSR1], bsR | axe, bsR, agg- |
| acaA- | acaA null mutant in Ax4 | axeA1, axeB1, axeC1, acaA-, [bsRcas], bsR | |
| acaA- | Homologous-recombination insertional knockout | axeA1, axeB1, axeC1, acaA-, [pACA-KO], bsR | |
| acaA-/acgA-/acrA- | Homologous-recombination insertional knockout | axeA1, axeB1, axeC1, acaA-, [pACA-KO], acgA-, [pACG-KO], acrA-, [pACR-KO], bsR | |
| acaA-/acgA-/acrA-/[act15]:pkaC:HA | pkaC overexpression under control of act15 promoter in adenylate-cyclase triple-null | axeA1, axeB1, axeC1, acaA-, acgA-, acrA-, [act15]:pkaC:HA, bsR, neoR | |
| acaA-/acgA-/acrA-/[act15]:pkaC:HA/[act15]:GFP | pkaC overexpression under control of act15 promoter and GFP-Lifeact, actin structure modified GFP, under control of act15 promoter in adenylate-cyclase triple-null | axeA1, axeB1, axeC1, acaA-, acgA-, acrA-, [act15]:pkaC:HA, [act15]:GFP, bsR, neoR, hygR | |
| acaA-/acgA-/acrA-/[act15]:pkaC:HA/[act15]:raf-RBD:GFP | pkaC overexpression under control of act15 promoter and raf-like ras binding domain (raf_RBD-GFP) under control of act15 promoter in adenylate-cyclase triple-null | axeA1, axeB1, axeC1, acaA-, acgA-, acrA-, [act15]:pkaC:HA, [act15]:raf-RBD:GFP, bsR, neoR, hygR | |
| acaA-/acgA-/acrA-/[act15]:pkaC:HA/[act15]:RFP:H2Bv3 | pkaC overexpression under control of act15 promoter and RFP-H2Bv3 (mCherry) under control of act15 promoter in adenylate-cyclase triple-null | axeA1, axeB1, axeC1, acaA-, acgA-, acrA-, [act15]:pkaC:HA, [act15]:RFP:2HBv3, bsR, neoR | |
| acaA-/[acaA-agg]:acaA:myc | myc tagged acaA expressed under control of acaA Pr1 (aggregation specific) promotor in acaA null cells | axeA1, axeB1, axeC1, acaA-, acaA, [bsRcas], bsR, neoR | |
| acaA-/[acaA-psp]:acaA:myc | myc tagged acaA expressed under control of acaA Pr2 promotor (active predominately in prespore cells) expressed in acaA null cells | axeA1, axeB1, axeC1, acaA-, acaA, [bsRcas], bsR, neoR | |
| acaA-/[acaA-pst]:acaA:myc | myc tagged acaA expressed under control of acaA Pr3 promotor (expressed predominately in prestalk tip cells) in acaA null cells | axeA1, axeB1, axeC1, acaA-, acaA, [bsRcas], bsR, neoR | |
| acaA-/[act15]:acaA | ACA under control of actin promotor expressed in aaA null cells | axeA1, axeB1, axeC1, acaA-, [pACA.URA], pyr5-6+, [pRG24], acaA, [pGSP10], neoR | |
| acaA-/[act15]:acaA(1-390;398-1407) | ACA with 7 residue deletion in the amino terminal portion of the C1 domain under control of actin promotor expressed in acaA null cells | axeA1, axeB1, axeC1, acaA-, [pACA.URA], pyr5-6+, [pRG24], acaA(1-390;398-1407), [pCP33], neoR | |
| acaA-/[act15]:acaA(L394T/K482N) | ACA with L394T/K482N substitutions under control of actin promotor expressed in acaA null cells | axeA1, axeB1, axeC1, acaA-, [pACA.URA], pyr5-6+, [pRG24], acaA(L394T/K482N), [pCP33], neoR | |
| acaA-/[act15]:acaA:YFP | acaA-YFP 3'''' fusion overexpressed in acaA- cells | axeA1, axeB1, axeC1, acaA-, [pACA.URA], pyr5-6+, [pRG24], YFP-acaA, neoR | |
| acaA-/[act15]:cap:GFP | CAP with C-terminal GFP expressed in acaA null | axeA2, axeB2, axeC2, acaA-, cap:GFP, [pflCAP-GFP], bsR, neoR | |
| acaA-/[act15]:HA:pkaC | acaA null mutant expressing HA tagged PkaC | axeA1, axeB1, axeC1, acaA-, [pACA.URA], pyr5-6+, [pRG24], HA:pkaC, [pDXA-3C], neoR | |
| acaA-/[act15]:HA:pkaC/acrA- | acaA/acrA double null mutant expressing HA tagged PkaC | acaA-/[act15]:HA:pkaC (DBS0350702) -Genotype: axeA1, axeB1, axeC1, acaA-, [pACA.URA], pyr5-6+, [pRG24], HA:pkaC, [pDXA-3C], acrA-, [pBSR1], bsR, neoR | |
| acaA-/[act15]:pkaC:HA | expressing PkaC under control of act15 promoter in acaA- | axeA1, axeB1, axeC1, acaA-/[act15]:pkaC:HA, bsR, neoR | |
| acaA-/[act15]:pkaR(G135E/G261A) | expression of PkaR that is unable to bind cAMP in acaA null | axeA1, axeB1, axeC1, acaA-, [pACA.URA], pyr5-6+, [pRG24], pkaR(G135E/G261A), neoR | |
| acaA-/[unk]:acaA(F306L/S498G | temperature sensitive mutant of AcaA with F306L/S498G,K504E,E593V,I649T substitutions expressed in acaA null cells | axeA1, axeB1, axeC1, acaA-, [pACA.URA], pyr5-6+, [pRG24], acaA(F306L/S498G, K504E, E593V, I649T), [pCP33], neoR | |
| acaA-/[unk]:acaA(N333D | AcaA with N333D,G633S point mutations expressed in acaA-
| axeA1, axeB1, axeC1, acaA-, [pACA.URA], pyr5-6+, [pRG24], acaA(N333D, G633S), [pCC33], neoR | |
| acaA-/[unk]:acaA(V427I | AcaA with V427I,S642F point mutations expressed in acaA- | axeA1, axeB1, axeC1, acaA-, [pACA.URA], pyr5-6+, [pRG24], acaA(V427I, S642F), [pCC33], neoR | |
| acaA-/[unk]:acgA | acgA expressed in acaA null mutant | axeA1, axeB1, axeC1, acaA-, [pACA.URA], pyr5-6+, [pRG24], acgA, neoR | |
| acaA-/[unk]:gcA(E440K/S502Q/H504D) | gca with E440K/S502Q/H504D substitutions which change catalytic activity to adenylyl cyclase, expressed in acaA null | axeA1, axeB1, axeC1, acaA-, [pACA.URA], pyr5-6+, [pRG24], gcA(E440K/S502Q/H504D), [pMB12N], bsR | |
| acapA- | acapA null mutant expressed in DH1-10 cells | axeA1, axeB1, axeC1, pyr5-6- [pRG24], ura-, [bsr-acapA], acapA-, bsR | |
| acapA- | acapA null mutant | axeA2, axeB2, axeC2, acapA-, [bsR-loxP], bsR | |
| acapA-/acapB- | acapA/acapB double null mutant | axeA2, axeB2, axeC2, acapA-, [bsR-loxP], acapB-, [loxP] | |
| acapA-/[act15]:acapA(R633Q):GFP | | axeA1, axeB1, axeC1, pyr5-6- [pRG24], ura-, [bsr-acapA], acapA-, [act15]:acapA(R633Q):GFP, bsR, neomycin | |
| acapA-/[act15]:acapA:GFP | GFP tagged AcapA under control of the act15 promotor over-expressed in acapA null cells | axeA1, axeB1, axeC1, pyr5-6- [pRG24], ura-, [bsr-acapA], acapA-, [pDXA-3C]acapA, bsR, neoR | |
| acapB- | acapB null mutant | axeA2, axeB2, axeC2, acapB-, [bsR-loxP], bsR | |
| acbA- | acbA null mutant | axeA1, axeB1, axeC1, acbA-[acbA-bsr], bsR | axe,bsR,poor spore vaibility |
| acbA-/tagA- | acbA-/tagA- double null mutant | axeA1, axeB1, axeC1, acbA-, tagA-, bsR | |
| acbA-/tagA-/[cotB]:lacZ | acbA-/tagA- double null mutant marked with lacZ under the control of the prespore promoter cotB | axeA1, axeB1, axeC1, acbA-, tagA-, [cotB]:lacZ, bsR, neoR | |
| acbA-/tagA-/[ecmA]:GFP | acbA-/tagA- double null mutant marked with GFP under the control of the prestalk promoter ecmA | axeA1, axeB1, axeC1, acbA-, tagA-, [ecmA]:GFP, bsR, neoR | |
| acbA-/tagA-/[ecmB]:lacZ | acbA-/tagA- double null mutant marked with lacZ under the control of the prestalk promoter ecmB | axeA1, axeB1, axeC1, acbA-, tagA-, [ecmB]:lacZ, bsR, neoR | |
| acbA-/[act15]:acbA | acbA overexpressor in acbA null mutant | axea1, axeB1, axeC1, acbA-[acbA-bsr], bsR, [A15::acbA/neo], neoR | axe,bsR,neoR |
| acbA-/[act15]:acbA(Y72A) | AbcA with a Tyr to Ala substitution at position 72 expressed in abcA null cells under control of the actin 15 promotor | axeA1, axeB1, axeC1, acbA-[acbA-bsr], act15:acbA(Y72A), [pDNeoII], bsR , neoR | |
| acbA-/[act15]:GFP | acbA null mutant marked with GFP under the control of the act15 promoter | axeA1, axeB1, axeC1, acbA-, [act15]:GFP, bsR, neoR | |
| acbA-/[act15]:tagA(1-890) | acbA null mutant overexpressing the protease domain of tagA under the control of the act15 promoter | axeA1, axeB1, axeC1, acbA-, [act15]:tagA(1-890), bsR, neoR | |
| acbA-/[cotB]:lacZ | acbA null mutant marked with lacZ under the control of the prespore promoter cotB | axeA1, axeB1, axeC1, acbA-, [cotB]:lacZ, bsR, neoR | |
| acbA-/[ecmA]:GFP | acbA null mutant marked with GFP under the control of the prestalk promoter ecmA | axeA1, axeB1, axeC1, acbA-, [ecmA]:GFP, bsR, neoR | |
| acbA-/[ecmB]:lacZ | acbA- null mutant marked with lacZ under the control of the prestalk promoter ecmB | axeA1, axeB1, axeC1, acbA-, [ecmB]:lacZ, bsR, neoR | |
| acgA- | acgA null mutant (probably not real null since acgA is located on duplication). | axeA1, axeB1, axeC1, acgA-[acg-KO], bsR | |
| acgA- | acgA null mutant | axeA2, axeB2, axeC2, acgA-, bsR | |
| acgA-/acrA- | Sequential homologous-recombination insertional knockout mediated by Cre-loxP recombination | axeA1, axeB1, axeC1, acrA-, [pACR-KO], acgA-, [pACG-, O], bsR | |
| acgA-/[acrA]:lacZ | acrA promoter driving the expression of lacZ in an acgA null mutant | axeA2, axeB2, axeC2, acgA-, bsR, [acrA]:lacZ, neoR | |
| acgRI4 | Random integrant of acg-KO plasmid | axeA1, axeB1, axeC1, [acg-KO], bsR | axe,bsR |
| acrA- | acrA null strain | axe, matA1, acrA-, [bsR-loxP], bsR | |
| acrA- | acrA null (adenylate cyclase) null mutant | axeA1, axeB1, axeC1, acrA-[pBSR1], bsR | axe, bsR, long stalks, very few spores |
| acrA-/acgA- | acrA/acgA double null mutant in Ax4 | axeA1, axeB1, axeC1, acrA-, [loxP], acgA-, [bsR-loxP], bsR | |
| acrA-/acgA-/acaA- | acrA/acgA/acaA triple null mutant in Ax4 | axeA1, axeB1, axeC1, acrA-, [loxP], acgA-, [loxP], acaA-, [bsR-loxP], bsR | |
| acrA-/acgA-/acaA-/[act15]:pkaC:myc | myc-tagged pkaC under control of act15 promotor expressed in acrA/acgA/acaA triple null mutant | axeA1, axeB1, axeC1, acrA-, [loxP], acgA-, [loxP], acaA-, [bsR-loxP], pkaC, [pDXA-HC], bsR, neoR | |
| acrA-/[act15]:acaA(L394S) | constitutive active AcaA with (L394S) substitution expressed in acrA null cells | axeA1, axeB1, axeC1, acrA-, [pBSR1], acaA(L394S), pCP105, bsR, neoR | |
| acrA-/[act15]:acgA(1-401) | a dominant negative acgA (residues 1-401) expressed in an acrA null mutant | axeA2, axeB2, axeC2, acrA-, bsR, [act15]:acgA(1-401), neoR | |
| acrA-/[act15]:acrA(D1010A) | AcrA with point mutation in phosphoryl-accepting residue expressed in acrA null strain; rescues the null phenotype | axe, matA1, acrA-, bsR, acrA(D1010A), neoR | |
| acrA-/[act15]:acrA(D1174A) | AcrA with point mutation in phosphoryl-accepting residue expressed in acrA null strain; rescues the null phenotype | axe, matA1, acrA-, bsR, acrA(D1174A), neoR | |
| acrA-/[act15]:acrA(N796D/D854A/G856A) | AcrA with point mutations in both catalytically active and ATP-binding residues expressed in acrA null strain; rescues the null phenotype | axe, matA1, acrA-, bsR, acrA(N796D/D854A/G856A), neoR | |
| acrA-/[act15]:acrA:YFP | YFP-marked AcrA expressed in acrA null strain; rescues the null phenotype | axe, matA1, acrA-, bsR, acrA:YFP, neoR | |
| acrA-/[pkaC]:pkaC | pkaC overexpressed under control of pkaC promotor in acrA null cells | axeA1, axeB1, axeC1, acrA, [pBSR1], pkaC, [pK-neo], bsR, neoR | |
| act15]:gpaB(K12A/L19A/I21A) | gpaB(K12A/L19A/I21A) expressed in KAX3 | axeA1, axeB1, axeC1, gpaB(K12A/L19A/I21A), [pDXA-GFP], neoR | |
| act5-/[act5]:RFP/NC4 | mCherry expressed under the act5 promoter replacing the actin 5 gene in NC4, which has no phenotypic relevance; in comparison to axenic strains growth and development are more robust | wt, act5-, RFP, [pDM1514], hygR | |
| Acytostelium anastomosans PP1 | Type specimen (Landolt #2604). | wt | wt |
| Acytostelium ellipticum | natural isolate | wt | wt |
| Acytostelium ellipticum AE2 (holotype) | natural isolate; available from ATCC (no. 22247). | wt | wt |
| Acytostelium irregularosporum TNS-C-0037 | Natural isolate.
ATCC no. 28401 | wt | |
| Acytostelium leptosomum | Natural isolate from Tennessee. | wt | wt |
| Acytostelium leptosomum FG12A | natural isolate | wt | wt |
| Acytostelium longisorophorum DB10A spp stock | Natural isolate from the Great Smoky Mountains National Park.
Type specimen (Landolt #1810). | wt | wt |
| Acytostelium magnisorum 08A | Natural isolate.
ATCC no. MYA-3270 | wt | wt |
| Acytostelium serpentarium SAB3A | Natural isolate.
ATCC no. MYA-3271 | wt | wt |
| Acytostelium singulare FD1B | Natural isolate.
ATCC no. MYA-3272 | wt | wt |
| Acytostelium sp. BB22A | Isolate from Great Smoky Mountains National Park. Landolt # 2608. | wt | wt |
| Acytostelium sp. GSE10A | Isolate from Great Smoky Mountains National Park. Landolt # 2614. | wt | wt |
| Acytostelium subglobosum | natural isolate; a different isolate of that strain is being sequenced, Acytostelium subglobosum LB1 | wt | wt |
| Acytostelium subglobosum LB1 | Natural isolate from the Philippines (1970); strain being sequenced by Hideko Urushihara | wt | wt |
| adcA- | adcA null strain | axeA1, axeB1, axeC1, adcA-, bsR | |
| adcB- | adcB null mutant expressed in AX2 cells | axeA2, axeB2, axeC2, adcB-, [bsRcas], bsR | |
