Strain Catalog

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Strain Descriptor Strain Summary Genotype Phenotype
gdcA-gdcA REMI mutant; the insertion site is 35 bp into the second exon; insertion at the upper case NlaIII restriction site (CATG) in this sequence: gatattaCATGtgaatatgg;
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axeA1, axeB1, axeC1, gdcA-, bsR
gdt1-gdt1 REMI mutant; growth-differentiation transition; uracil prototrophaxeA1, axeB1, axeC1, pyr5-6-[pRG24], gdt1-[DIV-2], ura+
gdt1-gdt1 null mutant (REMI recapitulation); growth-differentiation transition; blasticidin resistantaxeA2, axeB2, axeC2, gdt1-[2.9-Bsr-XbaI], bsR
gdt1-/gpaB-gdt1/gpaB double null mutant; blasticidin resistantaxeA2, axeB2, axeC2, gpaB-, gdt1-, bsR
gdt1-/[act15]:pkaR(G135E/G261A)gdt1 null mutant in a strain (Rm) expressing a constitutively active (G135E/G261A) pkaR (PKA regulatory subunit); blasticidin resistantaxeA2, axeB2, axeC2, pkaR(G135E/G261A), gdt1-, bsR
gdt2-gdt2-null (growth-differentiation transition mutant); blasticidin resistantaxeA2, axeB2, axeC2, gdt2-[pGDT2-bsr], bsRpremature development
gdt2-gdt2 null REMI mutant, originally isolated as propranol-resistant mutant; blasticidin resistantaxeA2, axeB2, axeC2, gdt2-[pBlast], bsR
gdt2-gdt2 REMI mutant; the insertion site is 434 bp upstream of the 3'''' boundary of the third exon; insertion at the upper case NlaIII restriction site (CATG) in this sequence: aaaatcacCATGagctaatgat;
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axeA1, axeB1, axeC1, gdt2-, bsR
gefA-gefA REMI mutant (aimless)axeA1, axeB1, axeC1, pyr5-6-[pRG24], gefA-[pRHI30], ura+axe,agg-
gefA-gefA null mutantaxeA1, axeB1, axeC1, gefA-
gefA-gefA null mutant in AX2axeA2, axeB2, axeC2, gefA-, [bsR-loxP], bsR
gefA-gefA null mutantaxeA1, axeB1, axeC1, gefA-[pRH155], pyr5-6-[pRG24], ura+
gefA-/ripA-gefA/ripA double null mutant
gefA-/[act]gefAgefA null mutant rescued with aleA cDNAaxeA1, axeB1, axeC1, gefA-[pRH155], pyr5-6-[pRG24], [p?], ura+, neoRaxe,ura+,neoR
gefA-/[dscB]:rasG(S17N)dominant-negative RasG expressed in gefA null cellsaxeA1, axeB1, axeC1, gefA-[pRH155], pyr5-6-[pRG24], ura-, rasG(S17N), neoR
gefB-/AX2gefB null mutant in AX2axeA2, axeB2, axeC2, gefB-, [bsRcas], bsR
gefB-/AX3RasgefB-null mutant. Does not grow in liquid: trying to do so will suppress the phenotype!axeA1, axeB1, axeC1, gefB-[gefB-bsr], bsRbsR
gefB-/rasS-gefB/rasS double null mutantbsR, neoR
gefC-gefC null mutantaxeA1, axeB1, axeC1, gefC-[gefC-bsr], bsR
gefD-gefD null mutantaxeA1, axeB1, axeC1, gefD-[gefD-bsr], bsR
gefE-gefE null mutantaxeA1, axeB1, axeC1, gefE-[gefE-bsr], bsRaxe,bsr,phototaxis defect
gefE-gefE null mutant in AX3axeA1, axeB1, axeC1, gefE-, [bsR-loxP], bsR
gefE-/uduB-gefE/uduB (rrgA) double null mutant in AX3axeA1, axeB1, axeC1, gefE-, [loxP], uduB-, [bsR-loxP], bsR
gefF:GFPinsertion of GFP at the C-terminus of the endogenous gefF gene in AX2axeA2, axeB2, axeC2, gefF:GFP, neoR
gefG-RasgefG-null mutantaxeA1, axeB1, axeC1, gefG-[gefG-bsr], bsRaxe, bsR
gefH-gefH null mutant in AX2axeA2, axeB2, axeC2, gefH-, [bsR-loxP], bsR
gefH-/gefA-gefH/gefA double null mutant in AX2axeA2, axeB2, axeC2, gefH-, [loxP], gefA-, [bsR-loxP], bsR
gefK-gefK null in AX3axeA1, axeB1, axeC1, gefK- bsR
gefK-/gacL-REMI mutant; note, the insertion site is in an intergenic region; 2,362 bp upstream of gefK and 1,026 bp upstream of gacLaxeA1, axeB1, axeC1, gefK-/gacL-, [pBSR1], bsR
gefL-gefL null mutantaxeA1, axeB1, axeC1, gefL-[gefL-bsr], bsRaxe,bsr,phototaxis defect
gefM-RasGEFM-null mutantaxeA2, axeB2, axeC2, gefM-, bsR
gefQ-gefQ null mutantaxeA2, axeB2, axeC2, gefQ- [neoR-gefQ], neoR
gefQ-/[act15]:GFP:gefQ(172-654)gefQ null cells expressing GFP-gefQ lacking the first 172 amino acids and the DEP and RasGEF domainsaxeA2, axeB2, axeC2, gefQ- [neoR-gefQ], GFP:gefQ(172-654), bsR, neoR
gefQ-/[act15]:GFP:gefQ(910-1298)gefQ null cells expressing GFP fused to the gefQ RasGEF domainaxeA2, axeB2, axeC2, gefQ- [neoR-gefQ], GFP:gefQ(910-1298), drugR?, neoR
gefR-gefR null mutant in AX2axeA2, axeB2, axeC2, gefR-, bsR
gefS-gefS null mutant in AX2 with a floxed Bsr cassetteaxeA2, axeB2, axeC2, gefS-[gefS-BSR], bsR
GEG04K11 (tmcB- in KAX3)GEG04 KO in KAX3 (tiny macrocyst = tmc); blasticidin resistantaxeA1, axeB1, axeC1, tmcB-[pGEG04-KO-KAX3], bsR
GEG10K23 (tmcC- in KAX3)GEG10 KO in KAX3 (gene involved in macrocyst formation); blasticidin resistantaxeA1, axeB1, axeC1, tmcC-[pGEG10-KO], bsR
gemA-gemA null strainaxeA2, axeB2, axeC2, gemA-, bsR
gflB-gflB null mutantaxeA1, axeB1, axeC1, gflB-, [bsR-cas], bsR
gflB-gflB knock-out in AX2axeA2, axeB2, axeC2, gflB-, [bsR-cas], bsR
gflB-/[act15]:GFP:gflB(380-1601gflB null mutant expressing the GAP and RasGEF domains, with an inactivating mutation in the RasGEF domain (T1180E).axeA1, axeB1, axeC1, gflB-, [bsR-cas], gflB(380-1601, T1180E), [pDM317], bsR, neoR
GFP-(N)-golvesin-(L9A/L10A)GFP-(N)-golvesin-(L9A,L10A) expressing cells (full-length golvesin lacking the di-leucine motif; partially impaired in retrieval).axeA2, axeB2, axeC2, [GFP-(N)-golvesin-(L9A, L10A)], neoRaxe, neoR
GFP-cFIPN-terminal GFP-cFIP (Glu1163 to Lys2038) fusion under the control of the actin 15 promoter; neomycin resistantaxeA2, axeB2, axeC2, GFP-cFIP[pDEX-GFP], neoR
GFP-cypEN-terminally tagged CypE in AX2axeA2, axeB2, axeC2, [pGFP-cypE], neoRaxe, neoR
GFP-FIPN-terminal GFP-FIP fusion under the control of the actin 15 promoter; neomycin resistantaxeA2, axeB2, axeC2, GFP-FIP[pDEX-GFP], neoR
GFP-grlEGFP-grlE overexpressed in AX2; neomycin resistantaxeA2, axeB2, axeC2, GFP-grlE [GFP-grlE], neoR
GFP-VwkAAX2 cells overexpressing GFP-VwkAaxeA2, axeB2, axeC2, [pTX-gfp-vwk], neoRaxe,neoR
GFP:pcnaGFP tagged pcna expressed in AX2 cellsaxeA2, axeB2, axeC2, act15:GFP:pcna, [pDEXH82], neoR
GFP:pcpGFP-tagged Pcp overexpressed in AX4 cellsaxeA1, axeB1, axeC1, [pDneo2a-GFP-pcp], neoR
GFP:phgA(1-274:283-438)/phgA-phgA- (drainin-) expressing GFP-phgA lacking 8 amino acids required for localization to the contractile vacuole (FLAAMFLL)axeA2, axeB2, axeC2, phgA-, unk:GFP:phgA(1-274:283-438), bsR, neoR
GFP:phgA/phgA-phgA- (drainin-) expressing GFP-phgAaxeA2, axeB2, axeC2, phgA-, unk:GFP:phgA, bsR, neoR
GFP:rapC(D18S)dominant negative rapC(D18S) expressed in KAX3axeA1, axeB1, axeC1, rapC(D18S)
GFP:rapC(G13V)constitutively active rapC(G13V) expressed in KAX3axeA1, axeB1, axeC1, rapC(G13V)
GFP:smasma tagged with GFP in AX3 axeA1, axeB1, axeC1, GFP:sma, neoR
ggtA-ggtA REMI mutantaxeA1, axeB1, axeC1, ggtA-, bsR
gip1-gip1 null mutant in AX2 background in which the BsR cassette has been removedaxeA2, axeB2, axeC2, gip1-, [loxP]
glcS-/DH1glcS null mutantaxeA1, axeB1, axeC1, pyr5-6-[pRG24], ura-, glcS-, bsRaxe, ura-, bsR
glcS-/HMXglcS- null mutant in HMX44A cellsaxe, tsg-901 and/or tsg-903, cyc-900, dif, glcS-, bsRaxe, bsR
glk-glk REMI mutant in AX4axeA1, axeB1, axeC1, glk-, [pBsrDBglII], bsR
glkA-glkA null mutant in AX2axeA2, axeB2, axeC2, glkA-, [bsRcas], bsR
glpD-glpD-axeA1, axeB1, axeC1, glpD-, neoR
GM101Germination mutant (lyophilized 22.4.75)ts
GM4gts40/HL501/G7gts/X55heterozygote diploid tester strain; Parents: GM4gts40, HL501, G7gts, and X55cycA/+, whiA/+, acrA/acrA, tsgD12/+, axeA1/+, axeB1/+, nagA211/+, gluA/+, manA2/manA2, tsg40
GM88Germination mutant (lyophilized 22.4.75)ts, grmts
gmd(G108D)fucosylation-negative mutant; has a Gly -> Asp mutation in the gmd geneaxeA1, axeB1, axeC1, gmd(G108D)axe
gmfA-gmfA null mutant in AX2axeA2, axeB2, axeC2, gmfA-, [bsRcas], bsR
gmsA-gmsA null mutant; blasticidin resistantaxeA1, axeB1, axeC1, gmsA-, bsR
gmsA-gmsA null mutant; blasticidin resistantgmsA-, bsR
GN1beta-glucosidase mutant, altered Km; tester heterozygous diploid strainaxeA1, axeC1, axeB1, gluAM1, nagA211, tsg
gnt1(1-253/H104D)catalytically dead gnt1 mutant; contains a point mutation (H104D) and a truncation after residue 253.xeA1, axeB1, axeC1, , gnt1(1-253/H104D), hygR
gnt14-gnt14 null mutant, deleting residues 323 to 479axeA2, axeB2, axeC2, gnt14-, bsR
gnt15-gnt15 null mutant, deleting residues 157 to 273axeA2, axeB2, axeC2, gnt15-, bsR
gnt15-gnt15 null mutant, deleting C-terminal residues 414 to 516axeA2, axeB2, axeC2, gnt15-, bsR
gnt15-/[act15]:FLAG:gnt15gnt15 null mutant clonee B12 overexpressing FLAG-gnt15axeA2, axeB2, axeC2, gnt15-, FLAG-gnt15, bsR, neoR
gnt15-/[act15]:FLAG:gnt15gnt15 null mutant clonee B8-5 overexpressing FLAG-gnt15axeA2, axeB2, axeC2, gnt15-, FLAG-gnt15, bsR, neoR
gnt2-gnt2 REMI mutantaxeA1, axeB1, axeC1, gnt2-, bsR
gol-gol REMI mutantaxeA1, axeB1, axeC1, gol-, bsR
gp130-gp130 null mutantaxeA1, axeB1, axeC1, pyr5-6-[pRG24], ura+, gp130-[gp130/pyr5-6]
GP138A-/GP138B-/GP138C-/GP138D-tetra knockout of GP138A-D in AX3 axeA1.axeB1, axeC1, GP138A-, GP138B-, GP138C-, GP138D-, bsR
GP138C-GP138C null mutant in AX3axeA1, axeB1, axeC1, bsR, GP138C-
gpaA-gpaA null mutantaxeA1, axeB1, axeC1, gpaA-, thy+
gpaA-gpaA (Gα1) null mutantaxeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, thy+
gpaA-/rpkA-gpaA (Galpha 1)/rpkA double null mutant axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, thy+, rpkA-, neoR
gpaA-/[cotC]:lacZgpaA null marked with lacZ under the control of the cotC promoteraxeA1, axeB1, axeC1, gpaA-, [cotC]:lacZ, neoR
gpaA-/[ecmA]:lacZgpaA null mutant marked with lacZ under the control of the prestalk ecmA promoteraxeA1, axeB1, axeC1, gpaA-, [ecmA]:lacZ, neoR
gpaA-/[ecmB]:lacZgpaA (G alpha 1) null mutant marked with lacZ under the control of the prestalk ecmB promoteraxeA1, axeB1, axeC1, gpaA-, [ecmB]:lacZ
gpaA-/[gpaA]:gpaA(G45V)overexpressor of gpaA(G45V) (constitutively active mutant) under the control of its own promoter in a multicopy extrachromosomal vector in gpaA null cellsaxeA1, axeB1, axeC1, gpaA-, [gpaA]:gpaA(G45V), neoR
gpaA-/[gpaA]:gpaA(Q206L)overexpressor of gpaA(Q206L) (constitutively active mutant) under the control of its own promoter in a multicopy extrachromosomal vector in gpaA null cells axeA1, axeB1, axeC1, gpaA-, [gpaA]:gpaA(Q206L), neoR
gpaB-Gα2 null mutant; the frigidA allele is gpaB (Coukell et al 1983)axeA1, axeB1, axeC1, gpaB-[pMYC14B], pyr5-6-[pRG24], ura+axe,agg-
gpaB-gpaB (Gα2) null mutantaxeA1, axeB1, axeC1, cycA1, tsgA1, acrD369, ebrB364, sprH351, manA1, tmpA600, gpaB-, thy+
gpaB-frigid mutant that has no detectable expression of gpaB (Galpha2)axeA2, axeB2, axeC2, tsgJ2, fgdA122 (formerly agg-122)axe,agg-
gpaB-frigid mutant that has no detectable expression of gpaB (Galpha2)axeA2, axeB2, axeC2, tsgJ2, fgdA104 (formerly aggI104)axe,agg-,tsg
gpaB-gpaB null mutantaxeA1, axeB1, axeC1, gpaB-, thy+
gpaB-/regA-gpaB/regA double null mutant axeA1, axeB1, axeC1, gpaB-, thy+, regA-[pregAKO], bsR
gpaB-/[act15]:gpaBgpaB null rescued with gpaB under the control of the act15 promoter (phenotype not rescued)axeA1, axeB1, axeC1, gpaB-, [act15/gpaB], neoR
gpaB-/[act15]:gpaBgpaB null mutant (Galpha2-) overexpressing gpaB axeA1, axeB1, axeC1, gpaB-[pMYC14B], pyr5-6-[pRG24], ura+ , /[act15/gpaB] , neoR
gpaB-/[act15]:gpaB(1-345):gpaD(337-345)gpaB null mutant (Galpha2-) overexpressing gpaB with its C terminus replaced with that of gpaD (Galpha4)axeA1, axeB1, axeC1, gpaB-[pMYC14B], pyr5-6-[pRG24], ura+ , [act15/gpaB(1-345):gpaD(337-345)] , neoR
gpaB-/[act15]:gpaB(C4A)gpaB with C4A substitution expressed in gpaB nullaxeA1, axeB1, axeC1, gpaB-[pMYC14B], pyr5-6-[pRG24], gpaB(C4A), ura+, neoR
gpaB-/[act15]:gpaB(D154V)gpaB(D154V) mutant overexpressed in gpaB null mutant axeA1, axeB1, axeC1, gpaB-[pMYC14B], pyr5-6-[pRG24], ura+, neoR, [act15]:gpaB(D154V)
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