Strain Descriptor | Strain Summary | Genotype | Phenotype |
---|---|---|---|
gdcA- | gdcA REMI mutant; the insertion site is 35 bp into the second exon; insertion at the upper case NlaIII restriction site (CATG) in this sequence: gatattaCATGtgaatatgg; get raw sequence data | axeA1, axeB1, axeC1, gdcA- | |
gdt1- | gdt1 REMI mutant; growth-differentiation transition; uracil prototroph | axeA1, axeB1, axeC1, pyr5- | |
gdt1- | gdt1 null mutant (REMI recapitulation); growth-differentiation transition; blasticidin resistant | axeA2, axeB2, axeC2, gdt1- | |
gdt1- | gdt1/gpaB double null mutant; blasticidin resistant | axeA2, axeB2, axeC2, gpaB- | |
gdt1- | gdt1 null mutant in a strain (Rm) expressing a constitutively active (G135E/G261A) pkaR (PKA regulatory subunit); blasticidin resistant | axeA2, axeB2, axeC2, pkaR(G135E/ | |
gdt2- | gdt2-null (growth-differentiation transition mutant); blasticidin resistant | axeA2, axeB2, axeC2, gdt2- | premature development |
gdt2- | gdt2 null REMI mutant, originally isolated as propranol-resistant mutant; blasticidin resistant | axeA2, axeB2, axeC2, gdt2- | |
gdt2- | gdt2 REMI mutant; the insertion site is 434 bp upstream of the 3'''' boundary of the third exon; insertion at the upper case NlaIII restriction site (CATG) in this sequence: aaaatcacCATGagctaatgat; get raw sequence data | axeA1, axeB1, axeC1, gdt2- | |
gefA- | gefA REMI mutant (aimless) | axeA1, axeB1, axeC1, pyr5- | axe,agg- |
gefA- | gefA null mutant | axeA1, axeB1, axeC1, gefA- | |
gefA- | gefA null mutant in AX2 | axeA2, axeB2, axeC2, gefA- | |
gefA- | gefA null mutant | axeA1, axeB1, axeC1, gefA- | |
gefA- | gefA/ripA double null mutant | ||
gefA- | gefA null mutant rescued with aleA cDNA | axeA1, axeB1, axeC1, gefA- | axe,ura+,neoR |
gefA- | dominant-negative RasG expressed in gefA null cells | axeA1, axeB1, axeC1, gefA- | |
gefB- | gefB null mutant in AX2 | axeA2, axeB2, axeC2, gefB- | |
gefB- | RasgefB-null mutant. Does not grow in liquid: trying to do so will suppress the phenotype! | axeA1, axeB1, axeC1, gefB- | bsR |
gefB- | gefB/rasS double null mutant | bsR, neoR | |
gefC- | gefC null mutant | axeA1, axeB1, axeC1, gefC- | |
gefD- | gefD null mutant | axeA1, axeB1, axeC1, gefD- | |
gefE- | gefE null mutant | axeA1, axeB1, axeC1, gefE- | axe,bsr,phototaxis defect |
gefE- | gefE null mutant in AX3 | axeA1, axeB1, axeC1, gefE- | |
gefE- | gefE/uduB (rrgA) double null mutant in AX3 | axeA1, axeB1, axeC1, gefE- | |
gefF: | insertion of GFP at the C-terminus of the endogenous gefF gene in AX2 | axeA2, axeB2, axeC2, gefF: | |
gefG- | RasgefG-null mutant | axeA1, axeB1, axeC1, gefG- | axe, bsR |
gefH- | gefH null mutant in AX2 | axeA2, axeB2, axeC2, gefH- | |
gefH- | gefH/gefA double null mutant in AX2 | axeA2, axeB2, axeC2, gefH- | |
gefK- | gefK null in AX3 | axeA1, axeB1, axeC1, gefK- | |
gefK- | REMI mutant; note, the insertion site is in an intergenic region; 2,362 bp upstream of gefK and 1,026 bp upstream of gacL | axeA1, axeB1, axeC1, gefK- | |
gefL- | gefL null mutant | axeA1, axeB1, axeC1, gefL- | axe,bsr,phototaxis defect |
gefM- | RasGEFM-null mutant | axeA2, axeB2, axeC2, gefM- | |
gefQ- | gefQ null mutant | axeA2, axeB2, axeC2, gefQ- | |
gefQ- | gefQ null cells expressing GFP-gefQ lacking the first 172 amino acids and the DEP and RasGEF domains | axeA2, axeB2, axeC2, gefQ- | |
gefQ- | gefQ null cells expressing GFP fused to the gefQ RasGEF domain | axeA2, axeB2, axeC2, gefQ- | |
gefR- | gefR null mutant in AX2 | axeA2, axeB2, axeC2, gefR- | |
gefS- | gefS null mutant in AX2 with a floxed Bsr cassette | axeA2, axeB2, axeC2, gefS- | |
GEG04K11 (tmcB- | GEG04 KO in KAX3 (tiny macrocyst = tmc); blasticidin resistant | axeA1, axeB1, axeC1, tmcB- | |
GEG10K23 (tmcC- | GEG10 KO in KAX3 (gene involved in macrocyst formation); blasticidin resistant | axeA1, axeB1, axeC1, tmcC- | |
gemA- | gemA null strain | axeA2, axeB2, axeC2, gemA- | |
gflB- | gflB null mutant | axeA1, axeB1, axeC1, gflB- | |
gflB- | gflB knock-out in AX2 | axeA2, axeB2, axeC2, gflB- | |
gflB- | gflB null mutant expressing the GAP and RasGEF domains, with an inactivating mutation in the RasGEF domain (T1180E). | axeA1, axeB1, axeC1, gflB- | |
GFP- | GFP-(N)-golvesin-(L9A,L10A) expressing cells (full-length golvesin lacking the di-leucine motif; partially impaired in retrieval). | axeA2, axeB2, axeC2, [GFP- | axe, neoR |
GFP- | N-terminal GFP-cFIP (Glu1163 to Lys2038) fusion under the control of the actin 15 promoter; neomycin resistant | axeA2, axeB2, axeC2, GFP- | |
GFP- | N-terminally tagged CypE in AX2 | axeA2, axeB2, axeC2, [pGFP- | axe, neoR |
GFP- | N-terminal GFP-FIP fusion under the control of the actin 15 promoter; neomycin resistant | axeA2, axeB2, axeC2, GFP- | |
GFP- | GFP-grlE overexpressed in AX2; neomycin resistant | axeA2, axeB2, axeC2, GFP- | |
GFP- | AX2 cells overexpressing GFP-VwkA | axeA2, axeB2, axeC2, [pTX- | axe,neoR |
GFP: | GFP tagged pcna expressed in AX2 cells | axeA2, axeB2, axeC2, act15: | |
GFP: | GFP-tagged Pcp overexpressed in AX4 cells | axeA1, axeB1, axeC1, [pDneo2a- | |
GFP: | phgA- (drainin-) expressing GFP-phgA lacking 8 amino acids required for localization to the contractile vacuole (FLAAMFLL) | axeA2, axeB2, axeC2, phgA- | |
GFP: | phgA- (drainin-) expressing GFP-phgA | axeA2, axeB2, axeC2, phgA- | |
GFP: | dominant negative rapC(D18S) expressed in KAX3 | axeA1, axeB1, axeC1, rapC(D18S) | |
GFP: | constitutively active rapC(G13V) expressed in KAX3 | axeA1, axeB1, axeC1, rapC(G13V) | |
GFP: | sma tagged with GFP in AX3 | axeA1, axeB1, axeC1, GFP: | |
ggtA- | ggtA REMI mutant | axeA1, axeB1, axeC1, ggtA- | |
gip1- | gip1 null mutant in AX2 background in which the BsR cassette has been removed | axeA2, axeB2, axeC2, gip1- | |
glcS- | glcS null mutant | axeA1, axeB1, axeC1, pyr5- | axe, ura-, bsR |
glcS- | glcS- null mutant in HMX44A cells | axe, tsg- | axe, bsR |
glk- | glk REMI mutant in AX4 | axeA1, axeB1, axeC1, glk- | |
glkA- | glkA null mutant in AX2 | axeA2, axeB2, axeC2, glkA- | |
glpD- | glpD- | axeA1, axeB1, axeC1, glpD- | |
GM101 | Germination mutant (lyophilized 22.4.75) | ts | |
GM4gts40/ | heterozygote diploid tester strain; Parents: GM4gts40, HL501, G7gts, and X55 | cycA/ | |
GM88 | Germination mutant (lyophilized 22.4.75) | ts, grm | ts |
gmd(G108D) | fucosylation-negative mutant; has a Gly -> Asp mutation in the gmd gene | axeA1, axeB1, axeC1, gmd(G108D) | axe |
gmfA- | gmfA null mutant in AX2 | axeA2, axeB2, axeC2, gmfA- | |
gmsA- | gmsA null mutant; blasticidin resistant | axeA1, axeB1, axeC1, gmsA- | |
gmsA- | gmsA null mutant; blasticidin resistant | gmsA- | |
GN1 | beta-glucosidase mutant, altered Km; tester heterozygous diploid strain | axeA1, axeC1, axeB1, gluAM1, nagA211, tsg | |
gnt1(1- | catalytically dead gnt1 mutant; contains a point mutation (H104D) and a truncation after residue 253. | xeA1, axeB1, axeC1, , gnt1(1- | |
gnt14- | gnt14 null mutant, deleting residues 323 to 479 | axeA2, axeB2, axeC2, gnt14- | |
gnt15- | gnt15 null mutant, deleting residues 157 to 273 | axeA2, axeB2, axeC2, gnt15- | |
gnt15- | gnt15 null mutant, deleting C-terminal residues 414 to 516 | axeA2, axeB2, axeC2, gnt15- | |
gnt15- | gnt15 null mutant clonee B12 overexpressing FLAG-gnt15 | axeA2, axeB2, axeC2, gnt15- | |
gnt15- | gnt15 null mutant clonee B8-5 overexpressing FLAG-gnt15 | axeA2, axeB2, axeC2, gnt15- | |
gnt2- | gnt2 REMI mutant | axeA1, axeB1, axeC1, gnt2- | |
gol- | gol REMI mutant | axeA1, axeB1, axeC1, gol- | |
gp130- | gp130 null mutant | axeA1, axeB1, axeC1, pyr5- | |
GP138A- | tetra knockout of GP138A-D in AX3 | axeA1.axeB1, axeC1, GP138A- | |
GP138C- | GP138C null mutant in AX3 | axeA1, axeB1, axeC1, bsR, GP138C- | |
gpaA- | gpaA null mutant | axeA1, axeB1, axeC1, gpaA- | |
gpaA- | gpaA (Gα1) null mutant | axeA1, axeB1, axeC1, pyr5- | |
gpaA- | gpaA (Galpha 1)/rpkA double null mutant | axeA1, axeB1, axeC1, pyr5- | |
gpaA- | gpaA null marked with lacZ under the control of the cotC promoter | axeA1, axeB1, axeC1, gpaA- | |
gpaA- | gpaA null mutant marked with lacZ under the control of the prestalk ecmA promoter | axeA1, axeB1, axeC1, gpaA- | |
gpaA- | gpaA (G alpha 1) null mutant marked with lacZ under the control of the prestalk ecmB promoter | axeA1, axeB1, axeC1, gpaA- | |
gpaA- | overexpressor of gpaA(G45V) (constitutively active mutant) under the control of its own promoter in a multicopy extrachromosomal vector in gpaA null cells | axeA1, axeB1, axeC1, gpaA- | |
gpaA- | overexpressor of gpaA(Q206L) (constitutively active mutant) under the control of its own promoter in a multicopy extrachromosomal vector in gpaA null cells | axeA1, axeB1, axeC1, gpaA- | |
gpaB- | Gα2 null mutant; the frigidA allele is gpaB (Coukell et al 1983) | axeA1, axeB1, axeC1, gpaB- | axe,agg- |
gpaB- | gpaB (Gα2) null mutant | axeA1, axeB1, axeC1, cycA1, tsgA1, acrD369, ebrB364, sprH351, manA1, tmpA600, gpaB- | |
gpaB- | frigid mutant that has no detectable expression of gpaB (Galpha2) | axeA2, axeB2, axeC2, tsgJ2, fgdA122 (formerly agg- | axe,agg- |
gpaB- | frigid mutant that has no detectable expression of gpaB (Galpha2) | axeA2, axeB2, axeC2, tsgJ2, fgdA104 (formerly aggI104) | axe,agg-,tsg |
gpaB- | gpaB null mutant | axeA1, axeB1, axeC1, gpaB- | |
gpaB- | gpaB/regA double null mutant | axeA1, axeB1, axeC1, gpaB- | |
gpaB- | gpaB null rescued with gpaB under the control of the act15 promoter (phenotype not rescued) | axeA1, axeB1, axeC1, gpaB- | |
gpaB- | gpaB null mutant (Galpha2-) overexpressing gpaB | axeA1, axeB1, axeC1, gpaB- | |
gpaB- | gpaB null mutant (Galpha2-) overexpressing gpaB with its C terminus replaced with that of gpaD (Galpha4) | axeA1, axeB1, axeC1, gpaB- | |
gpaB- | gpaB with C4A substitution expressed in gpaB null | axeA1, axeB1, axeC1, gpaB- | |
gpaB- | gpaB(D154V) mutant overexpressed in gpaB null mutant | axeA1, axeB1, axeC1, gpaB- |
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