| Strain Descriptor |
Strain Summary |
Genotype |
Phenotype |
| gskA-/[act15]:GFP:tacA | gskA null mutant expressing GFP-tacA | axeA2, axeB2, axeC2, gskA-, GFP:tacA, bsR, neoR | |
| gskA-/[act15]:gskA(Y214F) | gskA null mutant expressing gskA with the Zak1 phosphorylation mutation Y214F | axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], gskA-, gskA(Y214F), bsR | |
| gskA-/[ecmA]:LacZ | gskA null cells expressing lacZ under control of the ecmA promoter | axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], gskA-[bsR-gskA], ecmA/lacZ, bsR | |
| gskA-/[ecmB]:LacZ | gskA null cells expressing lacZ under control of the ecmB promoter | axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], gskA-[bsR-gskA], ecmB/lacZ, bsR | |
| gskA-/[unk]:pikA(1-492(S7D/S11D/S109D/S113D/S177D/S181D)) | PikA membrane localization domain with serine to aspartic acid substitutions at 6 potential phosphorylation sites expressed in gskA- background | axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], gskA-[bsR-gskA], pikA(1-492(S7D/S11D/S109D/S113D/S177D/S181D)), bsR, neoR | |
| gst4- | gst4 null mutant in AX2 | axeA2, axeB2, axeC2, gst4-, [bsRcas], bsr | |
| GT78-FLoe | His-agtA overexpressed in AX3; neomycin resistant | axeA1, axeB1, axeC1, [His6-GT78], neoR | |
| GT78-Noe | His-agtA (N-terminal, residues 2-310) overexpressed in AX3; neomycin resistant | axeA1, axeB1, axeC1, [His6-GT78(2-310)], neoR | |
| gtaC- | gtaC null mutant | axeA1, axeB1, axeC1, gtaC-, bsR | |
| gtaC- | gtaC null mutant in AX2 | axeA2, axeB2, axeC2, gtaC-, [bsR-loxP], bsR | |
| gtaC-/[act15]:GFP:gtaC | N-terminal fusion of GFP to gtaC under the control of the act15 promoter expressed in a gtaC null mutant | axeA2, axeB2, axeC2, gtaC-, [pLPBLP], [act15:GFP:gtaC], neoR | |
| gtaC-/[act15]:GFP:gtaC(C500 | gtaC null mutant overexpressing GFP fused to gtaC with cysteine to serine substitutions at positiions 500,503,522,525 under the control of the act15 promoter | axeA2, axeB2, axeC2, gtaC-, [pLPBLP], [act15:GFP:gtaC(C500, 503, 522, 525S)], bsR, hygR | |
| gtaC-/[act15]:GFP:gtaC(T376A/S379 | gtaC null mutant overexpressing GFP fused N-terminal to gtaC with Cys to Ser substitutions at positions 500,503,522,525, and Thr/Ser residues mutated to Ala at positions 376/379/380 under the control of the act15 promoter | axeA2, axeB2, axeC2, gtaC-, [pLPBLP], [act15:GFP:gtaC(T376A/S379, 380A/C500, 503, 522, 525S)], bsR, hygR | |
| gtaC-/[act15]:gtaC:(468-496):GFP:gtaC | mutant overexpressing GFP fused to the N-terminus of gtaC with the addition of an exogenous putative nuclear localization signal (NLS) | axeA2, axeB2, axeC2, gtaC-, [pLPBLP], [act15:GFP:gtaC(468-496)], bsR, neoR | |
| gtaC-KD | hypomorphic mutant of gtaC: REMI insertion in the promoter of gtaC that results in >10-fold reduction in gtaC expression | axeA1, axeB1, axeC1, gtaC-KD, bsR | |
| gtaC-KD/[act15]:GFP:gtaC | hypomorphic mutant of gtaC: REMI insertion in the promoter of gtaC, expressing GFP-gtaC | axeA1, axeB1, axeC1, gtaC-KD, GFP:gtaC, bsR, neoR | |
| gtaG- | gtaG null mutant in AX4 | axeA1, axeB1, axeC1, gtaG-, [bsRcas], bsR | |
| gtaG- | gtaG null mutant | axeA1, axeB1, axeC1, gtaG-, [pGtaG-KO], bsR | |
| gtaG- | gtaG insertional mutant (recapituration of V10633) | axeA1, axeB1, axeC1, gtaG-, [pBSR1], bsR | |
| gtaI- | gtaI insertional mutant | axeA1, axeB1, axeC1, gtaI-, [pRDR0022], bsR | |
| gxcA- | gxcA (rasGEF1) null mutant | axeA1, axeB1, axeC1, gxcA-, bsR | |
| gxcAA- | Genome Wide Dictyostelium Insertion bank (GWDI) gxcAA- mutant; insertion at position 6196058, chr 3; used enzyme: Hsp92II | axeA1, axeB1, axeC1, gxcAA-, [bsRcas], bsR | |
| gxcB- | gxcB null mutant | axeA2, axeB2, axeC2, gxcB-, [bsR-loxP], bsR | |
| gxcC- | gxcC null mutant in AX3 background | axeA1, axeB1, axeC1, gxcC-, [bsRcas], bsR | |
| gxcC-/[act15]:GFP:gxcC | gxcC null mutant overexpressing N-terminal GFP tagged gxcC under the control of the act15 promoter | axeA1, axeB1, axeC1, gxcC-, [bsRcas], GFP:gxcC, [pDM317], bsR, neoR | |
| gxcDD- | gxcDD null mutant | axeA2, axeB2, axeC2, gxcDD-, bsR | |
| gxcKK- | Cheater mutant. Remi insertion in gxcKK | axeA1, axeB1, axeC1, gxcKK-, [pBSR1], bsR | |
| gxcP- | gxcP REMI mutant; the insertion site is 369 bp downstream of the start codon; insertion at the upper case NlaIII restriction site (CATG) in this sequence: gatatttCATGgaaaggg; also note that a different clone of this mutant is available (DBS0349542)
get raw sequence data
| axeA1, axeB1, axeC1, gxcP-, bsR | |
| gxcT- | gxcT null cells expressed in AX2 cells | axeA2, axeB2, axeC2, gxcT-, [bsR-loxP], bsR | |
| gxcT-/[act15]:GFP:gxcT | GFP-GxcT cells under control of act15 promotor expressed in gxcT null cells | axeA2, axeB2, axeC2, gxcT-, [pLPBLP], GFP:gxcT, [pJK1], bsR, neoR | |
| H3b-/H3c- | H3b/H3c double null mutant in AX2 | axeA2, axeB2, axeC2, H3b-, [bsRcas]H3c-, [bsRcas], bsR | |
| hatA-/[hatB]:hatB | Multiple mutant containing disrupted hatA and HatB over expressed in AX2 cells | axeA2, axeB2, axeC2, hatA-, hatB, [pDNeo2], neoR | |
| HAZ1 | Wild isolate, and putative new species from Cambridgeshire, UK. | wt | |
| HB3 | chemosensory mutant; the parent is the cycloheximide resistant NC4 derivative DdC | mat1, cyc, ts, agg | aggregation defective |
| hbx2- | hbx2 null mutant in kAX3 | axeA1, axeB1, axeC1, bsR, hbx2- | wild type |
| hbx2- | hbx2 null mutant in JH10 | axeA1, axeB1, axeC1, hbx2- | wild type |
| hbx2-/warA- | hbx2/warA- double null mutant in JH10 | axeA1, axeB1, axeC1, bsR, hbx2-, warA- | development arrests at mound stage |
| hbx4- | hbx4 null mutant | axeA1, axeB1, axeC1, hbx4-, [bsRcas], bsR | |
| hbx4-/[ecmAO]:lacZ | hxb4 null mutant expressing lacZ under the control of the prestalk ecmAO promoter | axeA1, axeB1, axeC1, hbx4-, [bsRcas], ecmAO]:lacZ, bsR, neoR | |
| hbx9- | hbx9 null mutant in AX2 | axeA2, axeB2, axeC2, hbx9-, [bsRcas], bsR | |
| hbx9-/[ecmA]:lacZ | hbx9 null mutant expressing LacZ under control of ecmA promotor in AX2 | axeA2, axeB2, axeC2, hbx9-, [bsRcas], lacZ, [pEcmA-Gal], neoR | |
| HC112 | frigid mutant,fgdA | tsgE13, sprA, acrA100, radE100, bwnA1, fgdA125 (formerly aggI125) | agg-,brown,tsg,metR |
| HC198 | frigid mutant (fgdD) | tsgE13, sprA, acrA100, radE100, bwnA1, fgdD140 | agg-,tsg,metR |
| HC213 | frigid mutant (fgdA) | tsgE13, sprA, acrA100, radE100, bwnA1, fgdA141 | agg-,tsg,metR,brown |
| HC275 | frigid mutant,fgdE | axeA2, axeB2, axeC2, tsgJ2, fgdE143 | axe,agg-,tsg |
| HC317 | frigid mutant (fgdC) | fgdC146, cycA5, bsgA5 | agg- |
| HC350 | agg-minus strain | agg-, acrA, bwnA, cobA, tsg, man+, cyc+ | agg-,metR,brown,cobR,tsg |
| HC351 | agg-minus strain | agg-, acrA, bwnA, tsg | agg-,metR,brown,tsg |
| HC390 | control transformant for HC392 | axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, [patB-KO], patB+, thy+ | axe,thy+ |
| HC479 | ncsA+ control transformant | axeA2, axeB2, axeC2, [ncsA-KO], bsR | |
| HC6 | tester strain (Rob Kay lab) | axeA2, axeB2, axeC2, tsg-2(tsgJ2) | axe,wt,tsg |
| HC6 | tester strain (Rich Kessin lab) | axeA2, axeB2, axeC2, tsg-2(tsgJ2) | axe,wt |
| HC91 | tester strain | tsgE13, sprA, acrA100, radE100, bwnA1, matA1 | wt,tsg,brown,metR,round spores |
| HCL | | axeA1, axeB1 axeC1, hcl, neoR | |
| hcpA- | Remake of hcpA- (Heterochromatin Protein null) with bsr-cassette removed (floxed and cre''d) | axeA2, axeB2, axeC2, hcpA-[hcpA-flox-cre] | axe,wt |
| hcpA- | hcpA null mutant | axeA2, axeB2, axeC2, hcpA-[hcpA-bsr], bsR | axe,bsR,wt |
| hcpA-/hcpB-/[act6]:GFP:hcpA | GFP-hcpA overexpressed in a hcpA/hcpB double knock-out; the double null is inviable; the GFP-marked strain in a null background allows cleaner localization studies | axeA2, axeB2, axeC2, hcpA-[hcpA-flox-cre], GFP-hcpA, hcpB-, neoR, bsR | |
| hcpA-/[act6]:GFP:hcpA | Expression of GFP-HcpA in a cre/loxP''d hcpA null mutant | axeA2, axeB2, axeC2, hcpA-[hcpA-flox-cre], GFP-hcpA, neoR | |
| hcpA-RFP | C-terminal HcpA-RFP fusion under the control of the act15 promoter expressed in wt | axeA2, axeB2, axeC2, hcpA-[hcpA-RFP], neoR | axe,neoR |
| hcpB- | heterochromatin protein B null mutant | axeA2, axeB2, axeC2, hcpB-[pGem-hcpB-bsr], bsR | axe,bsR,wt |
| HD11 | Control cell line HD11 (for the PLC-KO HD10) is a "random" integrant of the PLC-KO vector somewhere in the genome, but not in the PLC gene, and therefore has normal PLC activity. Both HD10 and HD11 were made with G418 selection, which is known to affect PIP2 levels, thus requiring an isogenic control. | axeA1, axeB1, axeC1, [pNeoPLCko], neoR | axe,wt,neoR |
| hdaB- | attempts to produce a hdaA null mutant failed | axeA2, axeB2, axeC2, hdaB-, [bsR-loxP], bsR | |
| HEAL | | axeA1, axeB1 axeC1, heal, neoR | |
| HEBC | | axeA1, axeB1 axeC1, hebc, neoR | |
| helC- | helC REMI mutan | axeA1, axeB1, axeC1, pyr5-6-[pRG24], helC-[pRH130], ura+ | |
| helC- | RNA helicase null (KO) | axeA1, axeB1, axeC1, pyr5-6-[pRG24], helC-[pHM444], ura+ | axe,loose mounds |
| helE- | helE REMI mutant | axeA1, axeB1, axeC1, helE-, bsR | |
| helF- | Putative RNA helicase
(dsRNA-binding protein) knock out | axeA2, axeB2, axeC2, helF-[helF-bsr], bsR | axe,bsR,wt |
| helF-GFP | C-terminal HelF-GFP fusion under the control of the A15 promoter | axeA2, axeB2, axeC2, helF-GFP[helF-GFP], neoR | axe, neoR |
| hephA- | hephA knock-out mutant in AX2 | axeA2, axeB2, axeC2, hephA-, [bsRcas], bsR | |
| hfnA- | cre-lox mutant of hfnA- | axeA1, axeB1, axeC1, hfnA-, [loxP] | |
| hfnA-/abpC- | abpC null expressed in hfnA null background | axeA1, axeB1, axeC1, hfnA-, [loxP], abpC-[pDE109], hygR | |
| hfnA-/[ecmO]:lacZ | hfnA null cells expressing lacZ under the control of the pre stalk ecmO promoter | axeA1, axeB1, axeC1, hfnA-, [loxP], lacZ, [pDdGal-17 (H-)], neoR | |
| HG1694 | Control strain for HG1764 producing free GFP. | axeA2, axeB2, axeC2, [GFP-neo], neoR | axe, neoR, gfp |
| HG302 | cAMPS-resistant mutant (aggregates and fruits in the presence of cAMPS). | axeA2.axeB2.axeC2, casA1000 | |
| HG302 | cAMPS-resistant mutant; low ePD & ePD inhibitor; NG mutagenesis; tester strain | matA1, casA1000, axeA1, axeC1, axeB1 | |
| HG5013 | Chemotaxis mutant (defective in motility response to folate). | axeA2, axeB2, axeC2, | axe |
| HG5125 | Chemotaxis mutant (defective in motility response to folate). | axeA2, axeB2, axeC2, | axe |
| HG5142 | Chemotaxis mutant (defective in motility response to folate). | axeA2, axeB2, axeC2, | axe,agg- |
| hgrA- | hgrA null mutant in type II congenic strain | axeA2, axeB2, axeC2, matA-, [bsRcas], matB, matC, hgrA-, [bsRcas], bsR | |
| hgrA-/KAX3 | hgrA null mutant in KAX3 | xeA1, axeB1, axeC1, hgrA-, [bsRcas], bsR | |
| hgrB- | hgrB null mutant in type II congenic strain | axeA2, axeB2, axeC2, matA-, [bsRcas], matB, matC, hgrB-, [bsRcas], bsR | |
| hgrB-/KAX3 | hgrB null mutant in KAX3 | axeA1, axeB1, axeC1, hgrB-, [bsRcas], bsR | |
| HH20 | methanol resistant variant of NP86, an axenic V12 from Keith Williams | axe, met | axe,metR |
| HH25 | | axeA1, axeB1, axeC1, tsg | axe,wt |
| HH309 | Axenic V12 derived from a germinated macrocyst. | axe, matA2 | axe |
| HH309/bsr | Blasticidin-resistant version of axenic V12 derived from a germinated macrocyst. | [pBSR3], bsR | axenic,bsR |
| HH31 | tester strain | cycA1, axeA1, axeB1, axeC1, tsgA1, bwn, cobA1 | brown,axe,cobR,cycR,tsg |
| HH31 | tester strain X36 selected for good growth in FM. | axeA1, axeB1, axeC1, cycA1, tsgA1, bwn, cobA1 | axe,brown,cycR,cobR,tsg |
| HH31/HL16 | heterozygote diploid tester strain; Parents:HH31, HL16(DBS0236211) | cycA5/+, stkA520/stkA501, axeA1/+, axeB1/+, tsgB1/+, cobA/+, bsgB500/+ | |
| HH31M | tester strain | axeA1, axeB1, axeC1, cycA1, tsgA1, bwn, cobA1 | brown,axe,cycR,cobR,tsg |
| HH33 | pyrimidine auxotroph | axeA1, axeB1, axeC1, tsg, pyr-300 | axe,ura-,tagg on SM |
| HH34 | pyrimidine auxotroph | axeA1, axeB1, axeC1, tsg, pyr-301 | axe,ura-,wt |
| HH36 | purine auxotroph | axeA1, axeB1, axeC1, tsg, pur-300 | axe,ade- in FM,wt |
| HH39 | purine auxotroph | axeA1, axeB1, axeC1, tsg, pur-302 | axe,wt,ade- |
| hipA+ | Non-homologous recombinant of the hipA gene of Dictyostelium (control strain), in AX2 | axeA2, axeB2, axeC2, bsR | axe, bsR |
| hipA+ | Non-homologous recombinant of the hipA gene of Dictyostelium (control strain), in DH1 | axeA1, axeB1, axeC1, pyr5-6-[pRG24], ura+, [pSP72-pyr-HipKO] | axe |
| hipA- | hipA null mutant in AX2 | axeA2, axeB2, axeC2, hipA-[pSP72BSR-HipKO], bsR | axe, bsR |
| hipA- | Knockout of the hipA gene of Dictyostelium in DH1. | axeA1, axeB1, axeC1, pyr5-6-[pRG24], ura+, hipA-[pSP72-pyr-HipKO] | axe |
| hipA-/epnA- | double knockout of hipA and epnA | axeA1, axeB1, axeC1, pyr5-6-[pRG24], ura+, hipA-, epnA- | |
