| Strain Descriptor |
Strain Summary |
Genotype |
Phenotype |
| docD-/docA- | docD and docA double null mutant expressed in AX2 cells | axeA2, axeB2, axeC2, docD-, [loxP], docA-[bsR-loxP], bsR | |
| dokA- | dokA null mutant in AX2 | axeA2, axeB2, axeC2, neoR, dokA- | |
| dotA- | dotA null strain | axeA2, axeB2, axeC2, dotA-, bsR | |
| DP72 | | mut, dp72 | |
| dpoA- | dpoA null mutant (development is resistant to lithium) | axeA2, axeB2, axeC2, dpoA-[pBlast-dpoA-Cla], bsR | |
| dpoA- | dpoA null mutant (development is resistant to lithium) | axeA2, axeB2, axeC2, dpoA-[pBlast-dpoA-Cla], bsR | |
| dpoA- | dpoA null mutant (development is resistant to lithium) | axeA2, axeB2, axeC2, dpoA-[pBlast-dpoA-Cla], bsR | |
| dpoA- | dpoA null mutant (development is resistant to lithium) | axeA2, axeB2, axeC2, dpoA-[pBlast-dpoA-Cla], bsR | |
| dpoA- | dpoA REMI mutant (development is resistant to lithium) | axeA2, axeB2, axeC2, dpoA-[pBlast], bsR | axe, bsR, Li+R |
| dpoA-/gskA- | dpoA/gskA double null mutant | axeA1, axeB1, axeC1, pyr5-6-[pRG24], gskA-[pHM338], dpoA-, ura+, bsR | |
| drcA- | drcA null mutant | axeA2, axeB2, axeC2, drcA-, [bsR-loxP], bsR | |
| drkA- | drkA REMI mutant; the insertion site is 241 bp downtream of the start codon; insertion at the upper case DpnII restriction site (GATC) in this sequence: taatgaaGATCaagatatat;
get raw sequence data | axeA1, axeB1, axeC1, drkA-, bsR | |
| drkC- | drkC null mutant | axeA2, axeB2, axeC2, drkC-, bsR | |
| drkC-/[act15]:drkC(1-497):YFP | C-terminal YFP fusion to drkC where the kinase domain has been deleted expressed in a null background; does not rescue the phagocytosis phenotype | axeA2, axeB2, axeC2, drkC-, drkC(1-497)-YFP, [pYU20], bsR, neoR | |
| drkC-/[act15]:drkC(451-750):YFP | C-terminal YFP fusion to the drkC kinase domain overexpressed in a null background | axeA2, axeB2, axeC2, drkC-, drkC(451-750)-YFP, [pYU20], bsR, neoR | |
| drkC-/[act15]:drkC(K518R):YFP | C-terminal YFP fusion to the drkC protein where a conserved lysine has been mutated to arginine in the kinase domain, overexpressed in a null background | axeA2, axeB2, axeC2, drkC-, drkC(K518R)-YFP, [pYU20], bsR, neoR | |
| drkC-/[act15]:drkC:YFP | C-terminal YFP fusion to drkC expressed in a null background; rescues the phagocytosis phenotype | axeA2, axeB2, axeC2, drkC-, drkC-YFP, [pYU20], bsR, neoR | |
| drnA- | apparently present on a duplication, and only one of the two drnA copies are knocked out (C. Hammann, personnal communication to dictyBase) | axeA2, axeB2, axeC2, drnA-[pDrnA-KO], bsR | axe, bsR |
| drnB- | drnB null mutant in AX2 background with the BsR cassette removed | axeA2, axeB2, axeC2, drnB-, [loxP] | |
| drnB-/[act15]:pre-miRNA | this represents 3 strains overexpressing either the ddi-mir-1176, ddi-mir-1177, ddi-mir-7095, or ddi-mir-7102 hairpin structure to study the processing into miRNAs | axeA2, axeB2, axeC2, drnB-, [loxP], pre-dd-mir, neoR | |
| drnB-/[act6]:lacZ | drnB null mutant marked with lacZ under the control of the act6 promoter | axeA2, axeB2, axeC2, drnB-[loxP], lacZ, [pDneo2a], neoR | |
| drnB-/[act6]:lacZ/[RNAi]lacZ | expression of a hairpin Beta-gal construct (antisense 1200-1858, sense 1359-1858 linked by a GFP loop) in a drnB null mutant expressing an act6 driven LacZ gene | axeA2, axeB2, axeC2, drnB-, [loxP], lacZ, [pDneo2a], RNAi-lacZ, neoR, bsR | |
| dscA(1-2):MS2x24:dscA(3-253) | 24 bacteriophage MS2 stem loop knock-in into the dscA gene in AX2 | axeA2, axeB2, axeC2, [pJRC36], bsR | axe,bsR |
| dscA(1-2):MS2x24:dscA(3-253) | 24 bacteriophage MS2 stem loop knock-in into the dscA gene in AX3 | axeA1, axeB1, axeC1, [pJRC36], bsR | axe, bsR |
| dscA- | dscA null mutant | | |
| dscA- | dscA null mutant in AX2 | axeA2, axeB2, axeC2, dscA-, [bsRcas], bsR | |
| dsd- | dsd null mutant in AX2 | axeA2, axeB2, axeC2, dsd-, [bsR-loxP], bsR | |
| dsgg(1-571:581-733) | disgorgin (dsgg) with a 9 amino acid deletion (LAAVFLLIL) | axeA2, axeB2, axeC2, dsgg(1-571:581-733) | |
| dsgg(1-571:581-733)/dsgg- | dsgg (disgorgin) with a 9 amino acid deletion (LAAVFLLIL) expressed in dsgg null mutant | axeA2, axeB2, axeC2, dsgg-, dsgg(1-571:581-733), bsR | |
| dsgg- | dsgg (disgorgin) null mutant | axeA2, axeB2, axeC2, dsgg-, bsR | |
| dsgg-/GFP:dsgg | GFP-disgorgin expressed in the dsgg null mutant | axeA2, axeB2, axeC2, dsgg-, unk:GFP:DDB_G0280955, bsR, neoR | |
| dsgg-/lvsA- | dsgg-/lvsA- double null mutant; supressor of dsgg- | axeA2, axeB2, axeC2, dsgg-, lvsA-, bsR | |
| dsgg-/lvsD- | dsgg-/lvsD- double null mutant; enhancer of dsgg- | axeA2, axeB2, axeC2, dsgg-, lvsD-, bsR | |
| dsgg-/OE]:dsgg(Delta-Fbox) | disgorging (dsgg) with a deletion of the F box overexpressed in dsgg null mutant | axeA2, axeB2, axeC2, dsgg-, unk:dsgg(Delta-Fbox), bsR, neoR | |
| dsgg-/[OE]:dsgg | dsgg overexpressor mutant in dsgg null mutant | axeA2, axeB2, axeC2, dsgg-, unk:dsgg, bsR, neoR | |
| dsgg-/[OE]:dsgg(Q551A) | disgorgin (dsgg) with a Q to A mutation inactivating the GAP activity overexpressed in dsgg null mutant | axeA2, axeB2, axeC2, dsgg-, unk:dsgg(Q551A), bsR, neoR | |
| dsgg-/[OE]:dsggR515A) | dsgg with a R to A mutation inactivating the GAP activity overexpressed in dsgg null mutant | axeA2, axeB2, axeC2, dsgg-, unk:dsgg(R515A), bsR, neoR | |
| dsgg-/[OE]:GFP:lvsD | overexpressor of GFP-marked lvsD in dsggnull mutant | axeA2, axeB2, axeC2, dsgg-, [OE]:GFP:lvsD, bsR, neoR | |
| dsgg-/[OE]:lvsA | overexpressor of lvsA in dsgg null mutant | axeA2, axeB2, axeC2, dsgg-, [act6]:GFP:lvsA, bsR, neoR | |
| dsgg-/[OE]:phgA | overexpressor of drainin in dsgg null mutant
| axeA2, axeB2, axeC2, dsgg-, unk:phgA, bsR, neoR | |
| dsgg-/[OE]:rab8A | overexpression of rab8A in dsgg- enhances the enlarged vacuoles phenotype of dsgg- | axeA2, axeB2, axeC2dsgg-, [OE]:rab8A, bsR, neoR | |
| dspA- | RAM: regulator of adhesion and motility mutants; dspA- REMI mutant | axeA2, axeB2, axeC2, dspA-, [bsRcas], bsR | |
| dstA- | Dd-STATa-minus cells | axeA1, axeB1, axeC1, dstA- [pSTATa-KO], bsR | axe, bsR, ura+, incomplete fruiting bodies |
| dstA- | dstA null mutant in AX2 | axeA2, axeB2, axeC2, dstA-, bsR | axe, bsR |
| dstA- | dstA null mutant in kAX3 | axeA1, axeB1, axeC1, dstA-, bsR | axe, bsR |
| dstA-/dstB- | dstA/dstB double null mutant in AX2 | axeA2, axeB2, axeC2, dstA-, dstB-, bsR, hygR | |
| dstA-/GFP:dstA(237-707):ORF+ | dstA null mutant in which the core fragment of dstA (aa 237 to 707) is fused to GFP and integrated in low copy number | axeA2, axeB2, axeC2, dstA-, [GFP:dstA(237-707):ORF+], bsR | |
| dstA-/GFP:dstA(237-707):ORF+/[act15]:2C | 2C gene overexpressed in dstA null mutant in which the core fragment of dtsA (aa 237 to 707) is fused to GFP | axeA2, axeB2, axeC2, dstA-, [GFP:dstA(237-707):ORF+], pLD1A15SN[act15/2C] | |
| dstA-/GFP:dstA(237-707):ORF+/[act15]:7E | 7E gene overexpressed in dstA null mutant in which the core fragment of dtsA (aa 237 to 707) is fused to GFP | axeA2, axeB2, axeC2, dstA-, [GFP:dstA(237-707):ORF+], pLD1A15SN[act15/7E] | |
| dstA-/GFP:dstA(237-707):ORF+/[act15]:DDB_G0276165 | DDB_G0276165 overexpressed in dstA null mutant in which the core fragment of dtsA (aa 237 to 707) is fused to GFP | axeA2, axeB2, axeC2, dstA-, [GFP:dstA(237-707):ORF+], pLD1A15SN[act15/DDB_G0276165] | |
| dstA-/GFP:dstA(237-707):ORF+/[act15]:DDB_G0282325 | DDB_G0282325 overexpressed in dstA null mutant in which the core fragment of dtsA (aa 237 to 707) is fused to GFP | axeA2, axeB2, axeC2, dstA-, [GFP:dstA(237-707):ORF+], pLD1A15SN[act15/DDB_G0282325] | |
| dstA-/GFP:dstA(237-707):ORF+/[act15]:hssA | hssA overexpressed in dstA null mutant in which the core fragment of dtsA (aa 237 to 707) is fused to GFP; partly rescues the developmental defect of dstA null | axeA2, axeB2, axeC2, dstA-, bsR, pLD1A15SN[act15/hssA] | |
| dstA-/GFP:dstA(237-707):ORF+/[act15]:hssB | hssB overexpressed in dstA null mutant in which the core fragment of dtsA (aa 237 to 707) is fused to GFP | axeA2, axeB2, axeC2, dstA-, [GFP:dstA(237-707):ORF+], pLD1A15SN[act15/hssB | |
| dstA-/GFP:dstA(237-707):ORF+/[OE]dutA(751-1322) | dstA null mutant in which the core fragment of dstA (aa 237 to 707) is fused to GFP and integrated in low copy number; culmination phenotype of dstA mutant supressed by overexpressing a fragment of the ncRNA dutA, nucleotides 751-1322. | axeA2, axeB2, axeC2, dstA-, [GFP:dstA(237-707):ORF+], [OE:dutA(751-1322)], bsR, neoR | |
| dstA-/[act15]:dstA | dstA null rescued by dstA expression under the act15 promoter in kAX3 | axeA1, axeB1, axeC1, dstA-, [act15/dstA] | |
| dstA-/[cprB]:dstA | dstA null rescued by dstA expressed under the control of the late aggregation promoter cprB in kAX3 | axeA1, axeB1, axeC1, dstA-, [cprB]:dstA | |
| dstA-/[ecmAO]:dstA | dstA null rescued by dstA expressed under the control of the prestalk promoter ecmAO in kAX3 | axeA1, axeB1, axeC1, dstA-, [ecmAO]:dstA | |
| dstB- | dstB null mutant in AX2 | axeA2, axeB2, axeC2, dstB-, bsR | |
| dstB- | STATb-null in AX2 | axeA2, axeb2, axeC2, dstB-, hygR | axe, hygR |
| dstB-/dstC- | dstB/dstC double null mutant in AX2 | axeA2, axeB2, axeC2, dstB-, dstC-, bsR | |
| dstB-/[act15]:dstB | dstB null expressing dstB under the control of the act15 promoter | axeA2, axeB2, axeC2, dstB-, [act15]:dstB, bsR, neoR | |
| dstB-/[act15]:dstB(L1025R) | dstB null expressing dstB(L1025R) (SH2 domain) under the control of the act15 promoter | axeA2, axeB2, axeC2, dstB-, [act15/dstB(L1025R)], bsR, neoR | |
| dstB-/[act15]:dstB(Y1143F) | dstB null expressing dstB(Y1143F) (predicted tyrosine phosphorylation site) under the control of the act15 promoter | axeA2, axeB2, axeC2, dstB-, [act15/dstB(Y1143F)], bsR, neoR | |
| dstC- | dstC null mutant in AX2 cells | axeA2, axeB2, axeC2, dstC-, [hygRcas], hygR | |
| dstC- | dstC-null in AX2 | axeA2, axeB2, axeC2, dstC-, hygR | axe, hygR |
| dtfA- | dtfA- in DH1 | axeA1, axeB1, axeC1, pyr5-6-[pRG24], ura+, dtfA- | |
| DTS1 | tester strain for development, stopsat aggs; | dts | |
| DU260 | | mut, du60 | |
| DU2889 | | mut, du2889 | |
| DU454 | | mut, du454 | |
| DU707 | | mut, du707 | |
| DU768 | | wt, du768 | |
| DU955 | | mut, du955 | |
| DU957 | | mut, du957 | |
| DU979 | | mut, du979 | |
| dupA- | dupA REMI mutant in AX4 | axeA1, axeB1, axeC1, dupA-, [pBsrDBglII], bsR | |
| dupA- | dupA null mutant | axeA1, axeB1, axeC1, dupA-, [bsRcas], bsR | |
| dutA- | dutA null mutant | axeA2, axeB2, axeC2, neoR, dutA- | |
| dwwA- | dwwA null mutant | axeA2, axeB2, axeC2, dwwA-, bsR | |
| dydA- | dydA null mutant | axeA1, axeB1, axeC1, dydA-, [bsRcas], bsR | |
| dydA-/dydA(1-132:226-1211) | dydA mutant with an amino acid deletion of 133-225 (deletion of the PH domain) | axeA1, axeB1, axeC1, dydA-, [bsRcas], dydA(1-132:226-1211), bsR | |
| dydA-/dydA(1-278:501-1211) | dydA mutant with an amino acid deletion of 279-500 (deletion of the CH domain) | axeA1, axeB1, axeC1, dydA-, [bsRcas], dydA(1-278:501-1211), bsR | |
| dydA-/dydA(1-4:91-1111:1197-1211) | dydA mutant with an amino acid deletion of 5-90 and 1112-1196 (deletion of the 2 RA domains) | axeA1, axeB1, axeC1, dydA-, [bsRcas], dydA(1-4:91-1111:1197-1211), bsR | |
| dydA-/dydA(1-586:703-1211) | dydA mutant with an amino acid deletion of 586-702 (deletion of the PRM) | axeA1, axeB1, axeC1, dydA-, [bsRcas], dydA(1-586:703-1211), bsR | |
| dydA-/dydA(S861A/T865A) | dydA mutant with point mutations S861A and T865A | axeA1, axeB1, axeC1, dydA-, [bsRcas], dydA(S861A/T865A), bsR | |
| dymA- | dymA null expressed in AX2 | axeA2, axeB2, axeC2, dymA-, bsr | |
| dymA- | dymA null mutant | axeA2, axeB2, axeC2, dymA-, bsR | |
| dymA-/ehd- | ehd/dymA double null mutant | axeA2, axeB2, axeC2, dymA-, ehd-, [pBlueScript], bsR | |
| dymA-/[dymA]:dymA | dymA null mutant rescued with dymA under control of the native promotor | axeA2, axeB2, axeC2, dymA-, [dymA]:dymA, bsR, neoR | |
| dymB- | dymA null expressed in AX2 | axeA2, axeB2, axeC2, dymB-, bsr | |
| dymB- | dymB null mutant | axeA2, axeB2, axeC2, dymB-, bsR | |
| E. coli 281 | bacterial strain Escherichia coli 281 (originally from Raper); biosafety level 1 (USA) | e.coli281 | |
| E. coli B/r | bacterial strain Escherichia coli B/r; biosafety level 1 (USA) | e.coliB/r | |
| E. coli B/r | bacterial strain Escherichia coli B/r; biosafety level 1 (USA) | e.coliB/r | |
| E. coli B/r neoR | bacterial strain Escherichia coli B/r, neoR; transformed with plasmid pUC4K; biosafety level 1 (USA) | e.coliB/r, neoR | |
| E. coli B/r neoR | bacterial strain Escherichia coli B/r-1, neoR; biosafety level 1 (USA) | e.coliB/r, neoR | |
| E. coli DB | bacterial strain Escherichia coli that has been found to be an excellent food source for both axenic and wild D. discoideum strains; strain can be grown almost exclusively at RT on SM/5 plates; strain has been identified as most closely related to E. coli by PCR using 16S primers and sequencing the resultant 400bp PCR product however, the strain has not been fully sequenced. | e.coliDB | |
| eb1- | eb1 null mutant | axeA2, axeB2, axeC2, eb1-, bsR | |
| ecmA- | ecmA null mutant | axeA2, axeB2, axeC2, ecmA-, neoR | |
| ecmB- | ecmB null mutant | axeA1, axeB1, axeC1, ecmB- | |
