| Strain Descriptor |
Strain Summary |
Genotype |
Phenotype |
| [act15]:rasD(S17N) | dominant negative rasD(S17N) expressed in KAX3 | axeA1, axeB1, axeC1, rasD(S17N) | |
| [act15]:rasG(S17N) | doxycycline-inducible, dominant negative RasG under the control of the act15 promoter in AX3 cells | axeA1, axeB1, axeC1, rasG(S17N), [pDM310], neoR | |
| [act15]:rbg3 | rbg3 overexpressed in AX2 cells under control of the act15 promotor | axeA2, axeB2, axeC2, rbg3, [pHK12neo], neoR | |
| [act15]:rblA | overexpressor of rblA under the control of the constitutive act15 promoter | axeA1, axeB1, axeC1, [act15]:rblA, neoR | |
| [act15]:regA(126-316):myc | RegA response regulator domain expressed in AX2 | axeA2, axeB2, axeC2, regA(126-316), [pPT46] | |
| [act15]:RFP | AX3-1 expressing RFP under the control of the act15 promoter | axeA1, axeB1, axeC1, [pTX-RFP], neoR | axe, neoR, rfp |
| [act15]:RFP/[act15]:myc:btg | AX2 cells over-expressing myc-tagged, RFP labelled Btg under control of the act15 promotor | axeA2, axeB2, axeC2, [act15]:myc:btg, [act15]:RFP, RFP, neoR | |
| [act15]:RFP:abpE | RFP-tagged AbpE expressed in JH10 under control of the act15 promotor | axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, RFP:abpE, [pDBsrXP-mRFPmars], bsR, thy+ | |
| [act15]:RFP:mtpn | RFP-tagged mtpn in AX3 | axeA1, axeB1, axeC1, RFP:mtpn, [mRFPmars] | |
| [act15]:rgaA | AX2 cells overexpressing rgaA under control of the act15 promotor | axeA2, axeB2, axeC2, [act15]:rgaA, neoR | |
| [act15]:rgbA(751-1072 | rgbA lacking the BAR domain in AX2 | axeA2, axeB2, axeC2, rgbA(751-1072, 1080-1284, 1517-1846), [pDM450] | |
| [act15]:rgbA(751-1072 | rgbA lacking the GEF domain in AX2 | axeA2, axeB2, axeC2, rgbA(751-1072, 1121-1317, 1505-1834), [pDM450] | |
| [act15]:rheb | rheb overexpressed in AX2 under control of act15 promotor | axeA2, axeB2, axeC2, rheb, [pA15], neoR | |
| [act15]:rheb:YFP | YFP-marked rheb expressed in AX2 cells under control of the act15 promotor | axeA2, axeB2, axeC2, rheb:YFP, neoR | |
| [act15]:rnp1A | DDB_G0284167 overexpressed in AX3(rep)ORF+ | axeA1, axeB1, axeC1, [pREP], rnp1A, neoR | |
| [act15]:rps4 | rps4 overexpressed in AX2 | axeA2, axeB2, axeC2, rps4, [pACT15XPT1], neoR | |
| [act15]:rtoA | rtoA overexpressor | axeA1, axeB1, axeC1, [A15/rtoA], neoR | axe,neoR |
| [act15]:rzpA | act15-rzpA overexpressor | axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, thy-, act15:rzpA, neoR | |
| [act15]:sadA(921-952) | AX3 cells overexpressing the SadA tail domain, residues 921-952 | axeA1, axeB1, axeC1, act15:sadA(921-952), neoR | |
| [act15]:sgf29:FLAG | sgf29 overexpressed in AX2 | axeA2, axeB2, axeC2, sgf29, [pDM304], neoR | |
| [act15]:sgkA:FLAG | sgkA overexpressor, FLAG tagged | axeA1, axeB1, axeC1, pyr5-6-[pRG24], [Ddp2-ORF-pyr5-6], ura+, [act15/sgkA:FLAG], neoR | axe, neoR |
| [act15]:sir2E | sir2E overexpressor under the control of the act15 prromoter | axeA2, axeB2, axeC2, [act15/sir2E], neoR | |
| [act15]:snfA(1-379):GFP | overexpressing the catalytic domain of the AMPK alpha subunit (snfA) fused to GFP; residues 3 and 374-379 are altered; P and VLPRGQ respectively. | axeA2, axeB2, axeC2, snfA(1-379), neoR | |
| [act15]:snfA(1-379):GFP/[AS]ndkC-1 | ndcC antisense mutant coexpressed with AMPK alpha subunit (snfA) catalytic domain overexpressor in AX2 | axeA2, axeB2, axeC2, snfA(1-379), [pPROF500], neoR | |
| [act15]:snfA:YFP | C-terminal YFP-tagged SnfA overexpressed in AX2 | axeA2, axeB2, axeC2, snfA:YFP, neoR | |
| [act15]:sod2 | sod2 overexpressed in KAX3 | axeA1, axeB1, axeC1, sod2, [pExp4(+)], neoR | |
| [act15]:sodA | sodA overexpressed in KAX3 | axeA1, axeB1, axeC1, sodA, [pExp4(+)], neoR | |
| [act15]:sodA/gcsA- | sodA overexpressor in gcsA null mutant | axeA1, axeB1, axeC1, gcsA-, bsR, [act15/sodA:FLAG], neoR | |
| [act15]:sodA:myc | myc-tagged sodA overexpressed in AX2 | axeA2, axeB2, axeC2, neoR, sodA-OE | |
| [act15]:spkA:FLAG | FLAG-tagged spkA overexpressed under the control of the constitutive act15 promoter | axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, thy-, [act15]:spkA:FLAG, neoR | |
| [act15]:T7:rasG(G12T) | Constitutively active, N-terminally T7-tagged? rasG with G12T substitution expressed in KAX3 | axeA1, axeB1, axeC1, T7:rasG(G12T), [pCFC5], neoR | |
| [act15]:tagA(1-890) | overexpressor of the protease domain of tagA under the control of the constitutive act15 promoter | axeA1, axeB1, axeC1, [act15/tagA(1-890)], neoR | |
| [act15]:teep1(254-865):GFP | teep1(254-865) lacking PH domains in AX2 | axeA2, axeB2, axeC2, teep1(254-865), [pDM323], neoR | |
| [act15]:tgrC1/AX4 | secretory overexpression of the extracellular region of tgrC1(AX4) | axeA1, axeB1, axeC1, tgrC1(AX4), [pDXA-A15], neoR | |
| [act15]:tgrC1/QS31 | Secretory overexpression of the extracellular region of tgrC1(QS31) | axeA1, axeB1, axeC1, tgrC1(QS31), [pDXA-A15], neoR | |
| [act15]:tgrC1/QS37 | Secretory overexpression extracellular region of tgrC1(QS37) | axeA1, axeB1, axeC1, tgrC1(QS37), [pDXA-A15], neoR | |
| [act15]:tgrC1/QS4 | Secretory overexpression of the extracellular region of tgrC1(QS4) | axeA1, axeB1, axeC1, tgrC1(QS4), [pDXA-A15], neoR | |
| [act15]:tgrC1/QS45 | Secretory overexpression of the extracellular region of tgrC1 (QS45) | axeA1, axeB1, axeC1, tgrC1(QS45), [pDXA-A15], neoR | |
| [act15]:topA(1-82):GFP | topA (amino acids 1 to 82) fused to GFP under the control of the act15 promoter; neomycin resistant | axeA2, axeB2, axeC2, [pPROF299], neoR | |
| [act15]:tor(212-2380):YFP | YFP-tagged tor(212-2380) overexpressed in AX2 | axeA2, axeB2, axeC2, tor(212-2380), neoR | |
| [act15]:torA | overexpressor of torA under the control of the constitutive act15 promoter in AX3 | axeA1, axeB1, axeC1, [act15]:torA, neoR | |
| [act15]:tpt1:YFP | YFP-tagged tpt1 expressed in AX2 | axeA2, axeB2, axeC2, tpt1:YFP, neoR | |
| [act15]:trrA | trrA overexpressor under the control of the act15 promoter | axeA1, axeB1, axeC1, [act15/trrA], neoR | |
| [act15]:tubA:GFP | GFP-α-tubulin producing cells | axeA2, axeB2, axeC2, [GFP-tubA], neoR | axe, neoR, gfp |
| [act15]:tubC:GFP | tubulinC-GFP expressed in AX2 | axeA2, axeB2, axeC2, [tubC-GFP], neoR | axe, neoR, gfp |
| [act15]:vacA:GFP | vacA with C-terminal GFP over-expressed in AX2 under the control of act15 promotor | axeA2, axeB2, axeC2, vacA, [pDM323], neoR | |
| [act15]:vacB:GFP | vacB with C-terminal GFP over-expressed in AX2 under the control of act15 promotor | axeA2, axeB2, axeC2, vacB, [pDM323], neoR | |
| [act15]:vacC:GFP | vacC with C-terminal GFP overexpressed in AX2 under the control of act15 promotor | axeA2, axeB2, axeC2, vacC, [pDM323], neoR | |
| [act15]:vasP | overexpression of vasP in vasP null cells; high expressor (400% of wild type expression level); neomycin resistant | axeA1, axeB1, axeC1, vasP[pEXP4(+)VasP], neoR | |
| [act15]:vatM:GFP | AX2 cells expressing VatM-GFP | axeA2, axeB2, axeC2, vatM-GFP, neoR | axe, neoR |
| [act15]:vatM:GFP | AX3-ORF+ cells expressing VatM-GFP | axeA1, axeB1, axeC1, pyr5-6-[pRG24], [Ddp2-ORF-pyr5-6], ura+, [vatM-GFP], neoR | axe,neoR |
| [act15]:vinB:GFP | wildtype AX3 cells overexpressing vinB-GFP | axeA1, axeB1, axeC1, [act15]:vinB:GFP, neoR | |
| [act15]:washc5(N457D):GFP | washc5 (strumpellin) with N457D substitution in AX2 under control of act15 promotor | axeA2, axeB2, axeC2, washc5(N457D), [pBsr-C1-GFP], bsR | |
| [act15]:washc5:GFP | washc5 (strumpellin) overexpressed in AX2 under control of act15 promotor | axeA2, axeB2, axeC2, washc5, [pBsr-C1-GFP], bsR | |
| [act15]:YFP:iqgC | N-terminal YFP marked iqgC under control of act15 promotor overexpressed in AX2 | axeA2, axeB2, axeC2, iqgC, [pDM304], neoR | |
| [act15]:YFP:myoC(G395A) | YFP-tagged myoC with G395A substitution in AX2 | axeA2, axeB2, axeC2, myoC(G395A), [pDXA-3H], neoR | |
| [act15]:YFP:myoE | N-terminal YFP to myosin-IE (myoE) fusion in AX3; overexpressor | axeA1, axeB1, axeC1, pyr5-6-[pRG24], [Ddp2-ORF-pyr5-6], ura+ [EYFP-myoE], neoR | axe,neoR, increased phagocytosis |
| [act15]:YFP:myoJ | YFP-MyoJ expressed in wild type | axea2, axeB2, axeC2, YFP:myoJ, neoR | |
| [act15]:YFP:wipA | YFP-wipA overexpressed in wild-type | axeA1, axeB1, axeC1, [act15]:YFP:wipA, neoR | |
| [act15]:YFP:wipA(K36A) | K36A mutation in the WH2 domain of wipA, fused to YFP at the N-terminus, overexpressed in wild-type | axeA1, axeB1, axeC1, [act15]:YFP:wipA(K36A), neoR | |
| [act15]:YFP:wipA(K93A/S95A) | conserved residues (in WH1-like domain) K93A and S95A mutations in wipA, fused to YFP at the N-terminus, overexpressed in wild-type | axeA1, axeB1, axeC1, [act15]:YFP:wipA(K93A/S95A), neoR | |
| [act15]:[AS]dia2 | dia2 antisense mutant | axeA2, axeB2, axeC2, neoR, [act15]:[AS]dia2 | axe, neoR |
| [act15]:[myr]:pakA | overexpressor of pakA mutant containing NH2-terminal myristoylation signal from Src | axeA1, axeB1, axeC1, [act15]:[myr]:pakA, neoR | |
| [act5]:MS2-24:H3a(K5A) | histone H3a with K5A substitution knock-in into act5 in AX2
| axeA2, axeB2, axeC2, H3a(K5A), neoR | |
| [act6]:6xMyc:detA | myc-tagged detA in wild type | AxeA2, axeB2, axeC2, myc(6)-detA-, neoR | |
| [act6]:abpD(33-262) | abpD truncated at both the N and C-terminal regions expressed in AX2 | axeA2, axeB2, axeC2, abpD(33-262), [pDNeo2], neoR | |
| [act6]:canA | 50 x overexpression of calcineurin A (canA) in wild type | axeA2, axeB2, axeC2, canA [pDNeoII-canA], neoR | |
| [act6]:csaA | csaA overexpressed in KAX3 under control of act6 promotor | axeA1, axeB1, axeC1, [act6]:csaA, [pDNeo2], neoR | |
| [act6]:dia1 | dia1 overexpressor | axeA2, axeB2, axeC2, [act6]:dia1, neoR | |
| [act6]:dia2:GFP | dia2-GFP expressed in AX2 under the control of the act6 promoter | axeA2, axeB2, axeC2, [act6/dia2-gfp], neoR | axe, neoR, gfp |
| [act6]:GFP/P. pallidum | P. pallidum marked with GFP | [A6-GFP], neoR | neoR |
| [act6]:GFP:cepA/cepA(183-346) | knockout of endogenous cepA in GFP-tagged cepA overexpressing strain | axeA2, axeB2, axeC2, GFP:cepA, [pIS77], cepA(183-346), bsR, neoR | |
| [act6]:GFP:cepJ | knock-in of cepJ (mtaA) fused with superglow GFP under the control of the act6 promoter; overexpressor in vegetative cells, underexpressor when grown on bacteria | axeA2, axeB2, axeC2, superglowGFP:cepJ, bsR | |
| [act6]:GFP:cepL | cepL overexpressed in AX2 under control of Act6 promotor | axeA2, axeB2, axeC2, cepL, [pIS77], neoR | |
| [act6]:GFP:culD | GFP marked culD in wild type | AxeA2, axeB2, axeC2, GFP-culD-, neoR | |
| [act6]:GFP:detA | GFP marked detA in wild type | AxeA2, axeB2, axeC2, GFP-detA-, neoR | |
| [act6]:GFP:gefQ(S431A) | gefQ with S431A substitution which abolishes binding to 14-3-3 protein expressed in AX2 | axeA2, axeB2, axeC2, gefQ(S431A), [pDneo2a-GFP], neoR | |
| [act6]:GFP:lmnB(1-712) | N-terminal GFP tagged lmnB in which the last 4 amino acids (CLIM) have been removed, constituting the CaaX box. | axeA2, axeB2, axeC2, GFP:lmnB(1-712), [pAK35 ], neoR | |
| [act6]:GFP:lmnB(1-712)/S122A | N-terminal GFP tagged lmnB in which the last 4 amino acids (CLIM) have been removed, constituting the CaaX box; in addition, the predicted CDK1 phosphorylation site Serine 122 has been mutated to Alanine. | axeA2, axeB2, axeC2, GFP:lmnB(1-712), [pAK35], S122A[pPB14-29], neoR | |
| [act6]:GFP:lmnB(1-712 | N-terminal GFP tagged lmnB in which the last 4 amino acids (CLIM) have been removed constituting the CaaX box , and a point mutation of S122A (predicted CDK1 phosphorylation site) | axeA2, axeB2, axeC2, GFP:lmnB (1-712, S122A), [pAK35 ], neoR | |
| [act6]:GFP:orcB | overexpression of GFP-orcB in wild type | axeA2, axeB2, axeC2, [pDneoGFP-OrcB], neoR | |
| [act6]:GFP:rab2A | N-terminal GFP-tagged rab2A in AX4 cells | axeA1, axeB1, axeC1, GFP:rab2A, [pDneo2a-GFP], neoR | |
| [act6]:GFP:rab2A(N118I) | N-terminal GFP-tagged rab2A with a N118I substitution (dominant-negative) in AX4 cells | axeA1, axeB1, axeC1, GFP:rab2A(N118I), [pDneo2a-GFP], neoR | |
| [act6]:GFP:rab2A(Q64L) | N-terminal GFP-tagged rab2A with a Q64L substitution (constitutively active) in AX4 cells | axeA1, axeB1, axeC1, GFP:rab2A(Q64L), [pDneo2a-GFP], neoR | |
| [act6]:GFP:repE | GFP marked repE in wild type | AxeA2, axeB2, axeC2, GFP-repE-, neoR | |
| [act6]:GFP:sun1 | GFP-sun1 overexpressed in wild type | axeA2, axeB2, axeC2, GFP-sun1, neoR | |
| [act6]:GFP:sun1(1-722) | GFP-sun1 missing the SUN domain (residues 723-905), overexpressed in wild type | axeA2, axeB2, axeC2, GFP-sun1(1-722), neoR | |
| [act6]:GFP:vacA | GFP-VacA under the control of the actin 6 promoter expressed in wild-type, neomycin resistant | axeA2, axeB2, axeC2, GFP-vacA [pDNeoII], neoR | |
| [act6]:GFP:vacB(25-347:362-592) | GFP-VacB (starting at amino acid 25), hydrophobic residues 348 to 361 deleted, under the control of the actin 6 promoter expressed in wild-type, neomycin resistant
| axeA2, axeB2, axeC2, GFP-vacB(delta348-361)[pDNeoII-174], neoR | |
| [act6]:GFP:vacB(25-491) | GFP-VacB (starting at amino acid 25) truncated at the C-terminus to delete the coiled-coil domain, under the control of the actin 6 promoter expressed in wild-type, neomycin resistant | axeA2, axeB2, axeC2, GFP-vacB(25-491)[pDNeoII-175], neoR | |
| [act6]:GFP:vacB(25-555) | GFP-VacB (starting at amino acid 25) truncated at the C-terminus under the control of the actin 6 promoter expressed in wild-type, neomycin re | axeA2, axeB2, axeC2, GFP-vacB(25-555)[pDNeoII-275], neoR | |
| [act6]:GFP:vacB(474-555) | GFP-VacB residues 474-555 under the control of the actin 6 promoter expressed in wild-type, neomycin resistant | axeA2, axeB2, axeC2, GFP-vacB(474-555)[pDNeoII-264], neoR | |
| [act6]:GFP:vacB(474-592) | GFP-VacB truncated at the N-terminus, expressing residues 474-592 under the control of the actin 6 promoter, expressed in wild-type, neomycin resistant | axeA2, axeB2, axeC2, GFP-vacB(474-592)[pDNeoII-226], neoR | |
| [act6]:GFP:vacB(474-592) | GFP-VacB (starting at amino acid 25) under the control of the actin 6 promoter expressed in wild-type, neomycin resistant | axeA2, axeB2, axeC2, GFP-vacB[pDNeoII-112], neoR | |
| [act6]:gsk3/P.pallidum | P. pallidum gsk3 overexpressed in wild type; dictyBase Gene(s): PPA_G1293824
| pp, gsk3, [pPp-GSK3-OE], neoR | |
| [act6]:mrps4 | rps4 overexpressing cells (mitochondrial ribosomal protein S4) | axeA2, axeB2, axeC2, [rps4-OE vector], neoR | axe,neoR |
| [act6]:myc:eppA(S250A) | myc-tagged eppA with an S250A point mutation under the control of the act6 promoter in HS176 | axeA1, axeB1, axeC1, pyr5-6-[pΔPYR-BglII], [p240ClaI], ura+, [pDNeoII-myc-eppA(S250A)], neoR | |
| [act6]:myc:eppA(S325A) | myc-tagged eppA with an S325A point mutation under the control of the act6 promoter in HS176 | axeA1, axeB1, axeC1, pyr5-6-[pΔPYR-BglII], [p240ClaI], ura+, [pDNeoII-myc-eppA(S325A)], neoR | |
| [act6]:ptpB | overexpressor of ptpB under the control of the act6 promoter | axeA1, axeB1, axeC1, act6:ptpB, [pDNeo67], neoR | |
| [act6]:ptpC | overexpressor of ptpC under the control of the act6 promoter | axeA1, axeB1, axeC1, [act6]:ptpC, neoR | |
