| Strain Descriptor | Strain Summary | Genotype | Phenotype |
|---|---|---|---|
| talA- | talA/TalB double null overexpressing Flag-tagged talB | axeA2, axeB2, axeC2, talA- | |
| talA- | GFP-tagged talB with K16A substitution in talA/talB double null mutant | axeA2, axeB2, axeC2, talA- | |
| talA- | talA null mutant over-expressing FLAG-tagged talB | axeA2, axeB2, axeC2, talA- | |
| talA- | gbpD under the control of the act15 promoter expressed in a talA null mutant; neomycin resistant | axeA1, axeB1, axeC1, pyr5- | |
| talA- | talin (talA) null mutant overexpressing a constitutively active form of rapA under the control of the act15 promoter | axeA1, axeB1, axeC1, [act15/ | |
| talA/ | REMI mutant; note, the insertion site is in an intergenic region; 633 bp downstream of talA, and 3015 bp downstream of DDB_G0290533;
get raw sequence data | axeA1, axeB1, axeC1, talA, DDB_G0290533, bsR | |
| talB- | talin B null mutant in AX2 | axeA2, axeB2, axeC2, talB- | axe, bsR |
| talB- | talin B null mutant in DH1 | axeA1, axeB1, axeC1, pyr5- | axe |
| talB- | talin B null mutant in HMX44A. | axe, tsg- | axe, bsR |
| talB- | talin B null mutant in DH1. | axeA1, axeB1, axeC1, pyr5- | axe, ura-, bsR |
| talB- | talB/pikB double null mutant in AX2 | axeA2, axeB2, axeC2, talB- | |
| talB- | talB null mutant over-expressing talA-GFP | axeA2, axeB2, axeC2, talB- | |
| talB- | GFP-tagged talB with K16A substitution in talB null mutant | axeA2, axeB2, axeC2, talB- | |
| tasA- | tasA/tasB double null mutant in Polysphondylium pallidum | matI, tasA- | |
| tasA- | tasA/B double null with the G418 cassette removed | matI, tasA- | |
| tasA- | TasB expressed under the control of its own promoter in the tasA-/tasB- double knockout mutant | matI, tasA- | |
| tasB- | tasB null mutant in Polysphondylium pallidum PN500 | matI, tasB- | |
| tat- | tat REMI mutant; the insertion site is 546 bp downstream of the 5'' boundary of the second exon; insertion at the upper case DpnII restriction site (GATC) in this sequence: tatcacGATCatgaattgca;
get raw sequence data | axeA1, axeB1, axeC1, tat- | |
| tbcrA- | tbcrA null mutant in AX2 | axeA2, axeB2, axeC2, tbcrA- | |
| teep1- | teep1 null mutant in AX2 | axeA2, axeB2, axeC2, teep1- | |
| tert- | tert null mutant in AX2 | axeA2, axeB2, axeC2, tert- | |
| tert- | tert null mutant in AX4 | axeA1, axeB1, axeC1, tert- | |
| tet7 | AX2 cells containing a plasmid (pMB35) with the transactivator domain,tTAs* driven by the act15 promoter; tet on/off system transactivator cell | axeA2, axeB2, axeC2, tTAs*, [pMB35], neoR | |
| tgrB1(G275D)/ | AX4 cells with an additional tgrB1 allele from AX4 strain with G275D activation mutation; co-expresses tdTomato under act15 promoter and superfolder GFP under cotB promoter | axeA1, axeB1, axeC1, tgrB1(G275D), RFP, [act15: | |
| tgrB1(G307)/ | AX4 cells with an additional tgrB1 allele from AX4 strain with G307D activation mutation; co-expresses tdTomato under act15 promoter and superfolder GFP under cotB promoter | axeA1, axeB1, axeC1, tgrB1(G307), RFP, [act15: | |
| tgrB1- | tgrB1 null mutant | axeA1, axeB1, axeC1, tgrB1- | |
| tgrB1- | tgrB1-null strain with hygromycin resistance | axeA1, axeB1, axeC1, tgrB1- | |
| tgrB1- | tgrB1-null strain with no antibiotic resistance. BSR cassete was looped out by Cre recombinase expression; floxed strain AK1505 | axeA1, axeB1, axeC1, tgrB1- | |
| tgrB1- | stcA REMI mutant in AX4 tgrB1/tgrC1 double gene replacement strain expressing tgrB1 and tgrC1 alleles from QS31 under the QS31 tgrB1/tgrC1 promoter; pyr56-null; 5-FOA selection; used to create chimeras. | axeA1, axeB1, axeC1, pyr5- | |
| tgrB1- | tgrB1-single gene replacement strain expressing tgrB1 allele from QS38 | axeA1, axeB1, axeC1, tgrB1- | |
| tgrB1- | tgrB1-single gene replacement strain expressing tgrB1 allele from QS38, and expressing GFP under the act15 promoter | axeA1, axeB1, axeC1, tgrB1- | |
| tgrB1- | tgrB1-single gene replacement strain expressing tgrB1 allele from QS38, and expressing RFP (tdTomato) under act15 promoter | axeA1, axeB1, axeC1, tgrB1- | |
| tgrB1- | tgrB1-single gene replacement strain expressing tgrB1 allele from QS45 | axeA1, axeB1, axeC1, tgrB1- | |
| tgrB1- | tgrB1-single gene replacement strain expressing tgrB1 allele from QS45, and expressing GFP under act15 promoter | axeA1, axeB1, axeC1, tgrB1- | |
| tgrB1- | tgrB1-single gene replacement strain expressing tgrB1 allele from QS45, and expressing RFP (tdTomato) under act15 promoter | axeA1, axeB1, axeC1, tgrB1- | |
| tgrB1- | tgrB1-single gene replacement strain expressing tgrB1 allele from QS4 | axeA1, axeB1, axeC1, tgrB1- | |
| tgrB1- | tgrB1-single gene replacement strain expressing tgrB1 allele from QS4, and expressing GFP under act15 promoter | axeA1, axeB1, axeC1, tgrB1- | |
| tgrB1- | tgrB1-single gene replacement strain expressing tgrB1 allele from QS4, and expressing RFP(tdTomato) under the act15 promoter | axeA1, axeB1, axeC1, tgrB1- | |
| tgrB1- | stcA REMI mutant in parental tgrB1 null cells | axeA1, axeB1, axeC1, tgrB1- | |
| tgrB1- | tgrB1-single gene replacement strain expressing tgrB1 allele from AX4 | axeA1, axeB1, axeC1, tgrB1- | |
| tgrB1- | The resident tgrB1 allele was replaced with a tgrB1 gene where the cytoplasmic domain between amino acids 828 and 902 was deleted; with a C-terminal HA-epitope added | axeA1, axeB1, axeC1, tgrB1- | |
| tgrB1- | tgrB1-single gene replacement strain expressing tgrB1 allele from AX4, and expressing GFP under act15 promoter | axeA1, axeB1, axeC1, tgrB1- | |
| tgrB1- | tgrB1-single gene replacement strain expressing tgrB1 allele from AX4, and expressing RFP(tdTomato) under act15 promoter | axeA1, axeB1, axeC1, tgrB1- | |
| tgrB1- | tgrB1/tgrC1 double null strain with the pyr56 gene inserted in the tgrB1-C1 locus; the pyr56 gene is deleted from native locus and inserted in tgrB1 and tgrC1 locus | axeA1, axeB1, axeC1, pyr5- | |
| tgrB1- | merodiploid strain with an additional tgrB1 AX4 allele in a tgrB1/C1(QS31) double gene replacement background | axeA1, axeB1, axeC1, pyr5- | |
| tgrB1- | merodiploid strain witha an additional tgrB1 allele of AX4 with C-terminal truncation (828-902) and an HA tag in tgrB1/tgrC1(QS31) double gene replacement background; tdTomato (RFP) is expressed under the act15 promoter; superfolder GFP is expressed under the cotB promoter | axeA1, axeB1, axeC1, pyr5- | |
| tgrB1- | merodiploid strain with an additional tgrB1 AX4 allele in a tgrB1/C1(QS31) double gene replacement background; expresses GFP under act15 promoter | axeA1, axeB1, axeC1, pyr5- | |
| tgrB1- | merodiploid strain with an additional tgrB1 allele of AX4 with C-terminal HA-tag in tgrB1/tgrC1(QS31) double gene replacement background; tdTomato (RFP) is expressed under the act15 promoter; superfolder GFP is expressed under the cotB promoter | axeA1, axeB1, axeC1, pyr5- | |
| tgrB1- | merodiploid strain with an additional tgrC1 AX4 allele in a tgrB1/C1(QS31) double gene replacement background | axeA1, axeB1, axeC1, pyr5- | |
| tgrB1- | merodiploid strain with an additional tgrC1 AX4 allele in a tgrB1/C1(QS31) double gene replacement background; expresses GFP under act15 promoter | axeA1, axeB1, axeC1, pyr5- | |
| tgrB1- | tgrB1-/tgrC1- double knock-out expressing the alg9ins mutation ; insertion is 19 nt upstream of the alg9 gene | axeA1, axeB1.axeC1, pyr5- | |
| tgrB1- | AX4 tgrB1/tgrC1 double gene replacement strain expressing tgrB1 and tgrC1 alleles from QS31 under the QS31 tgrB1/tgrC1 promoter; pyr56-null (ura-); 5-FOA selection. | axeA1, axeB1, axeC1, pyr5- | |
| tgrB1- | tgrB1/tgrC1 double gene replacement strain expressing tgrB1 and tgrC1 alleles from QS31 under the QS31 tgrB1/tgrC1 promoter; expressing GFP under the act15 promoter; pyr56-null, 5FOA resistant | axeA1, axeB1, axeC1, pyr5- | |
| tgrB1- | AX4 tgrB1/tgrC1 double gene replacement strain expressing tgrB1 and tgrC1 alleles from QS38 under the QS38 tgrB1/tgrC1 promoter; pyr56-null, 5-FOA selection. | axeA1, axeB1, axeC1, pyr5- | |
| tgrB1- | tgrB1/tgrC1 double gene replacement strain expressing tgrB1 and tgrC1 alleles from QS38 under the QS38 tgrB1/tgrC1 promoter; expressing GFP under the act15 promoter; pyr56-null, 5FOA resistant | axeA1, axeB1, axeC1, pyr5- | |
| tgrB1- | tgrB1/tgrC1 double gene replacement strain expressing tgrB1 and tgrC1 alleles from QS38 under the QS38 tgrB1/tgrC1 promoter; expressing RFP(tdTomato) under the act15 promoter; pyr56-null, 5FOA resistant | axeA1, axeB1, axeC1, pyr5- | |
| tgrB1- | tgrB1/tgrC1 double gene replacement strain expressing tgrB1 and tgrC1 alleles from QS31 under the QS31 tgrB1/tgrC1 promoter; expressing RFP(tdTomato) under the act15 promoter; pyr56-null; 5FOA resistant | axeA1, axeB1, axeC1, pyr5- | |
| tgrB1- | AX4 tgrB1/tgrC1 double gene replacement strain expressing tgrB1 and tgrC1 alleles from QS45 under the QS45 tgrB1/tgrC1 promoter; pyr56-null, 5-FOA selection. | axeA1, axeB1, axeC1, pyr5- | |
| tgrB1- | tgrB1/tgrC1 double gene replacement strain expressing tgrB1 and tgrC1 alleles from QS45 under the QS45 tgrB1/tgrC1 promoter; expressing GFP under the act15 promoter; pyr56-null, 5FOA resistant | axeA1, axeB1, axeC1, pyr5- | |
| tgrB1- | tgrB1/tgrC1 double gene replacement strain expressing tgrB1 and tgrC1 alleles from QS45 under the QS45 tgrB1/tgrC1 promoter; expressing RFP(tdTomato) under the act15 promoter; pyr56-null; 5FOA resistant | axeA1, axeB1, axeC1, pyr5- | |
| tgrB1- | AX4 tgrB1/tgrC1 double gene replacement strain expressing tgrB1 and tgrC1 alleles from QS4 under the QS4 tgrB1/tgrC1 promoter; pyr56-null (ura-), 5-FOA selection. | axeA1, axeB1, axeC1, pyr5- | |
| tgrB1- | merodiploid strain with an additional tgrC1 allele of QS31 in tgrB1/tgrC1(QS4) double gene replacement background | axeA1, axeB1, axeC1, pyr5- | |
| tgrB1- | tgrB1/tgrC1 double gene replacement strain expressing tgrB1 and tgrC1 alleles from QS4 under the QS4 tgrB1/tgrC1 promoter; expressing GFP under the act15 promoter; pyr56-null, 5FOA resistant | axeA1, axeB1, axeC1, pyr5- | |
| tgrB1- | tgrB1/tgrC1 double gene replacement strain expressing tgrB1 and tgrC1 alleles from QS4 under the QS4 tgrB1/tgrC1 promoter; expressing RFP(tdTomato) under act15 promote; rpyr56-null | axeA1, axeB1, axeC1, pyr5- | |
| tgrB1- | tgrB1-/tgrC1 double gene replacement strain; AX4 alleles; tgrB1 gene has C-terminal truncation(828-902) that corresponds to intracellular domain and C-terminal HA tag; the pyr56 gene that was inserted in the double KO strain was deleted in the process and renders these cells uracil auxotroph | axeA1, axeB1, axeC1, pyr5- | |
| tgrB1- | tgrB1-/tgrC1 double gene replacement strain; AX4 alleles; tgrB1 gene with C-terminal truncation(828-902) that corresponds to intracellular domain and C-terminal HA tag; labeled with GFP ; uracil auxotroph | axeA1, axeB1, axeC1, pyr5- | |
| tgrB1- | tgrB1-/tgrC1- double gene deletion strain expressing an AX4 tgrB1 allele with a G275D activation mutation | axeA1, axeB1, axeC1, tgrB1- | |
| tgrB1- | tgrB1-/tgrC1- double gene deletion strain expressing an AX4 tgrB1 allele with a G307D activation mutation | axeA1, axeB1, axeC1, tgrB1- | |
| tgrB1- | tgrB1-/tgrC1- double gene deletion strain expressing an AX4 tgrB1 allele with a L846F activation mutation | axeA1, axeB1, axeC1, tgrB1- | |
| tgrB1- | tgrB1/tgrC1 double gene replacement strain expressing tgrB1 and tgrC1 alleles from AX4; pyr56-null, 5FOA resistant | axeA1, axeB1, axeC1, pyr5- | |
| tgrB1- | tgrB1/tgrC1 double gene replacement strain expressing tgrB1 and tgrC1 alleles from AX4; expressing GFP under the act15 promoter; pyr56-null (ura-), 5FOA resistant | axeA1, axeB1, axeC1, pyr5- | |
| tgrB1- | tgrB1/tgrC1 double gene replacement strain expressing tgrB1 and tgrC1 alleles from AX4; expressing RFP(tdTomato) under the act15 promoter; pyr56-null; 5FOA resistant | axeA1, axeB1, axeC1, pyr5- | |
| tgrB1- | tgrB1-/tgrC1 double gene replacement strain; AX4 alleles; tgrB1 gene has a C-terminal HA tag; the pyr56 gene that was inserted in the double KO strain was deleted in the process and renders these cells uracil auxotroph | axeA1, axeB1, axeC1, pyr5- | |
| tgrB1- | tgrB1-/tgrC1- double gene replacement strain; genes are from AX4 allele; tgrB1 has C-terminal HA tag; co-expresses tdTomato under act15 promoter and superfolder GFP under cotB promoter; expresses GFP only when co-developing with AX4 | axeA1, axeB1, axeC1, pyr5- | |
| tgrB1- | tgrB1-/tgrC1 double gene replacement strain; AX4 alleles; tgrB1 gene has a C-terminal HA tag; labeled with GFP ; these cells are uracil auxotroph | axeA1, axeB1, axeC1, pyr5- | |
| tgrB1- | tgrB1/tgrC1 double null strain with the pyr56 gene inserted in the tgrB1-C1 locus; the pyr56 gene is deleted from native locus and inserted in tgrB1 and tgrC1 locus; expressing GFP under the act15 promoter | axeA1, axeB1, axeC1, tgrB1- | |
| tgrB1- | tgrB1/tgrC1 double null strain with the pyr56 gene inserted in the tgrB1-C1 locus; the pyr56 gene is deleted from native locus and inserted in tgrB1 and tgrC1 locus; expressing RFP(tdTomato) under the act15 promoter | axeA1, axeB1, axeC1, tgrB1- | |
| tgrB1- | tgrB1 null mutant expressing GFP under the act15 promoter | axeA1, axeB1, axeC1, tgrB1- | |
| tgrB1/ | merodiploid strain expressing tgrB1 and tgrC1 alleles of AX4 in the native locus and containing an extra copy of both tgrB1 and tgrC1 alleles from AX4 inserted randomly in the genome; expressing RFP(tdTomato) under the act15 promoter | axeA1, axeB1, axeC1, tgrB1, tgrC1, RFP, [act15: | |
| tgrC1- | tgrC1 REMI mutant; lacks membrane glycoprotein gp150 | axeA1, axeB1, axeC1, pyr5- | |
| tgrC1- | tgrC1 null mutant in AX4 | axeA1, axeB1, axeC1, tgrC1- | |
| tgrC1- | mutant suppressing the developmental phenotype of the tgrC1- mutant expressing the tgrC1 allele from QS38; insertion is 19 nt upstream of the alg9 gene | axeA1, axeB1, axeC1, tgrC1- | |
| tgrC1- | recapitulated mutant suppressing the tgrC1 developmental phenotype; insertion is 19 nt upstream of the alg9 gene | axeA1, axeB1, axeC1, tgrC1- | |
| tgrC1- | REMI mutant suppressing the tgrC1 developmental phenotype; insertion is 19 nt upstream of the alg9 gene | axeA1, axeB1, axeC1, tgrC1- | |
| tgrC1- | tgrC1-single gene replacement strain expressing tgrC1 allele from QS31 | axeA1, axeB1, axeC1, tgrC1- | |
| tgrC1- | tgrC1-single gene replacement strain expressing tgrC1 allele from QS31, and expressing GFP under act15 promoter | axeA1, axeB1, axeC1, tgrC1- | |
| tgrC1- | tgrC1-single gene replacement strain expressing tgrC1 allele from QS31, and expressing RFP (tdTomato) under act15 promoter | axeA1, axeB1, axeC1, tgrC1- | |
| tgrC1- | tgrC1-single gene replacement strain expressing tgrC1 allele from QS38, and expressing GFP under act15 promoter | axeA1, axeB1, axeC1, tgrC1- | |
| tgrC1- | tgrC1-single gene replacement strain expressing tgrC1 allele from QS38, and expressing RFP (tdTomato) under act15 promoter | axeA1, axeB1, axeC1, tgrC1- | |
| tgrC1- | tgrC1-single gene replacement strain expressing tgrC1 allele from QS45 | axeA1, axeB1, axeC1, tgrC1- | |
| tgrC1- | tgrC1-single gene replacement strain expressing tgrC1 allele from QS45, and expressing GFP under act15 promoter | axeA1, axeB1, axeC1, tgrC1- | |
| tgrC1- | tgrC1-single gene replacement strain expressing tgrC1 allele from QS45, and expressing RFP (tdTomato) under act15 promoter | axeA1, axeB1, axeC1, tgrC1- | |
| tgrC1- | tgrC1-single gene replacement strain expressing tgrC1 allele from QS4 | axeA1, axeB1, axeC1, tgrC1- | |
| tgrC1- | tgrC1-single gene replacement strain expressing tgrC1 allele from QS4, and expressing GFP under act15 promoter | axeA1, axeB1, axeC1, tgrC1- | |
| tgrC1- | tgrC1-single gene replacement strain expressing tgrC1 allele from QS4, and expressing RFP (tdTomato) under act15 promoter | axeA1, axeB1, axeC1, tgrC1- | |
| tgrC1- | stcA REMI mutant (supressor) in parental tgrC1 null cells; the insertion site is 78 bp upstream of the stcA stop codon | axeA1, axeB1, axeC1, tgrC1- | |
| tgrC1- | stcB REMI mutant (supressor) in parental tgrC1 null cells | axeA1, axeB1, axeC1, tgrC1- | |
| tgrC1- | stcC REMI mutant (supressor) in parental tgrC1 null cells | axeA1, axeB1, axeC1, tgrC1- | |
| tgrC1- | tgrC1-single gene replacement strain expressing tgrC1 allele from AX4 | axeA1, axeB1, axeC1, tgrC1- | |
| tgrC1- | tgrC1-single gene replacement strain expressing tgrC1 allele from AX4, and expressing RFP(tdTomato) under the act15 promoter | axeA1, axeB1, axeC1, tgrC1- |
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