| Strain Descriptor |
Strain Summary |
Genotype |
Phenotype |
| [act15]:ifkA(881-1514(K1258G | ifkA kinase domain with activating substitutions (K/G at residue 1258, F/L at residue 1320) fused to E. coli gyrB gyrase domain and expressed in AX4 cells | axeA1, axeB1, axeC1, ifkA(881-1514(K1258G, F1320L)), [pEXP4(+)], neoR | |
| [act15]:lacZ | HL328 (uracil auxotroph) cells expressing lacZ under the control of the constitutive act15 promoter | axeA1, axeB1, axeC1, pyr5-6-[pDPYR-BglII], ura-, [actin15::lacZ], neoR | axe,ura-,neoR |
| [act15]:lacZ | actin15-lacZ marked strain (blue everywhere) | axeA1, axeB1, axeC1, [act15::lacZ], neoR | axe,neoR |
| [act15]:mcln:GFP | mcln overexpressed in AX2 | axeA2, axeB2, axeC2, mcln, [pPROF638], neoR | |
| [act15]:mekA(299-360) | AX3 cells overexpressing mekA with an N-terminal deletion of the region upstream from the catalytic core under the control of the actin 15 promoter | axeA1, axeB1, axeC1, [act15:mekA(299-360)], neoR | |
| [act15]:mekA(K321A) | AX3 cells overexpressing mekA with the lysine residue required for ATP binding converted to an alanine (K321A) under the control of the actin 15 promoter | axeA1, axeB1, axeC1, [act15:mekA(K321A)], neoR | |
| [act15]:mekA(S444A/T448A) | AX3 cells overexpressing mekA with double alanine substitutions (S444A/T448A) under the control of the act15 promoter | axeA1, axeB1, axeC1, [act15:mekA(S444A/T448A)], neoR | |
| [act15]:mhcA(1-871) | mhcA globular head domain (sub fragment 1, S1) in AX2 | axeA2, axeB2, axeC2, mhcA(1-871), [pDM51R], hygR | |
| [act15]:mhcA(2-762) | mhcA(2-762) in AX2 | axeA2, axeB2, axeC2, mhcA(2-762), [pB10TP2], neoR | |
| [act15]:mhkB:FLAG | overexpression of the FLAG epitope at the amino-terminus of mhkB in wild type | axeA2, axeB2, axeC2, mhkB-FLAG [pTX-MKB2], neoR | |
| [act15]:mip1/[act15]:mekA:FLAG | wild type overexpressing mipA and FLAG tagged mekA | axeA1, axeB1, axeC1, act15:mip1, act15:mekA:FLAG, neoR | |
| [act15]:mkkA | overexpressor of mkkA under the control of the act15 promoter | axeA1, axeB1, axeC1, [act15]:mkkA, neoR | |
| [act15]:mkkA(K199A) | overexpressor of mkkA(K199A) dominant-negative mutant under the control of the act15 promoter | axeA1, axeB1, axeC1, [act15]:mkkA(K199A), neoR | |
| [act15]:mkkA{deltaWD40} | overexpressor of mkkA{delta}WD repeat region under the control of the act15 promoter | axeA1, axeB1, axeC1, [act15]:mkkA, neoR | |
| [act15]:mkpA:GFP:TAP | C-terminal GFP and TAP-tagged MkpA expressed in AX4 cells under the control of the act15 promotor | axeA1, axeB1, axeC1, mkpA:GFP:TAP, [ pDV-CGFP-CTAP], neoR | |
| [act15]:mkpB(C294V) | catalytically inactive MkpB expressed in AX4 cells under control of the act15 promotor | axeA1, axeB1, axeC1, mkpB(C294V), [pDdGal-17], neoR | |
| [act15]:mkpB:GFP:TAP | C-terminal GFP and TAP-tagged MkpB expressed in AX4 cells under the control of the act15 promotor | axeA1, axeB1, axeC1, mkpB:GFP:TAP, [ pDV-CGFP-CTAP], neoR | |
| [act15]:mtaA(1-813)/[dscB]tubA:GFP | overexpressor of a C-terminal truncated mutant of mtaA (DdCP224) (mutant unable to bind to microtubules) marked with tubA-GFP under the control of the discoidin promoter | axeA2, axeB2, axeC2, [act15]:mtaA(1-813):GFP, neoR | |
| [act15]:myc-cdk8 | cdk8 overexpressor tagged with myc | axeA2, axeB2, axeC2, [act15]:myc-cdk8 | |
| [act15]:myc-cdk8(D194A) | cdk8 catalytic dead overexpressor tagged with myc | axeA2, axeB2, axeC2, [act15]:myc-cdk8(1-380) | |
| [act15]:myc:cdk8/[act15]:FLAG:cycC | myc-tagged cdk8 and FLAG-tagged CycC over expressed in AX2 cells, both under control of the act15 promotor | axeA2, axeB2, axeC2, [act15:myc-cdk8], [act15:FLAG:cycC], neoR | |
| [act15]:myc:mlcE | AX3 strain expressing myc-tagged mlcE. | axeA1, axeB1, axeC1, [pmycELC], neoR | axe, neoR |
| [act15]:myc:ptpC | overexpression of myc tagged ptpC under the control of the act15 promoter | axeA2, axeB2, axeC2, act15:myc:ptpC, neoR | |
| [act15]:myc:ptpC | Constitutive overexpression of myc tagged ptpC in the KAX3 background, under the control of the act15 promoter | axeA1, axeB1, axeC1, [act15]:myc:ptpC, neoR | |
| [act15]:myc:ptpCΔ(C649S) | overexpression of myc tagged ptpC(C649S), catalytically inactive, with 244 amino acids deletion of a region near the amino terminus of the protein,
under the control of the act15 promoter | axeA2, axeB2, axeC2, act15:myc:ptpCΔ(C649S), neoR | |
| [act15]:myc:ptpCΔC649S | Overexpression of myc tagged, catalytically inactive ptpC(C649S) in the KAX3 background, veder the control of the act15 promoter | axeA1, axeB1, axeC1, [act15]:myc:ptpCΔ(C649S), neoR | |
| [act15]:myc:ptpC(C649S) | overexpression of myc tagged ptpC(C649S), catalytically inactive, under the control of the act15 promoter | axeA2, axeB2, axeC2, act15:myc:ptpC(C649S), neoR | |
| [act15]:myc:rapA | overexpressor rapA tagged with myc under the control of the act15 promoter | axeA1, axeB1, axeC1, [act15/myc:rapA], neoR | |
| [act15]:myc:rapA(G12V) | overexpressor of a constitutively active form of rapA tagged with myc under the control of the act15 promoter | axeA1, axeB1, axeC1, [act15/myc:rapA(G12V)], neoR | |
| [act15]:myc:rapA(S17N) | overexpressor of a dominant negative form of rapA tagged with myc under the control of the act15 promoter | axeA1, axeB1, axeC1, [act15/myc:rapA(S17N)], neoR | |
| [act15]:myc:rckA | overexpression of rckA with an N-terminal myc tag under the control of the constitutive act15 promoter | axeA1, axeB1, axeC1, [myc-rckA-OE], neoR | |
| [act15]:myc:rckA(C551A) | overexpression of rckA (C551A) with an N-terminal myc tag under the control of the constitutive act15 promoter | axeA1, axeB1, axeC1, [myc-rckA(C551A)-OE], neoR | |
| [act15]:myc:rckA(E510G) | overexpression of rckA (E510G) with an N-terminal myc tag under the constitutive act15 promoter | axeA1, axeB1, axeC1, [myc-rckA(E510G)-OE], neoR | |
| [act15]:myc:rckA(E510G/N511A) | overexpression of rckA (E510G/N511A) with an N-terminal myc tag under the control of the constitutive act15 promoter | axeA1, axeB1, axeC1, [myc-rckA(E510G/N511A)-OE], neoR | |
| [act15]:myc:rckA(K867A) | overexpression of a kinase dead rckA (K867A) with an N-terminal myc tag under the control of the constitutive act15 promoter | axeA1, axeB1, axeC1, [myc-rckA(K867A)-OE], neoR | |
| [act15]:myc:splB | SplB under control of the act15 promotor overexpressed in AX2 cells | axeA2, axeB2, axeC2, [act15]:myc:splB, neoR | |
| [act15]:myc:talA(2255-2492)/puf118(677-1036) | myc-tagged talA residues 2255-2492 fused with residues 677-1036 of puf118 expressed in AX2 | axeA2, axeB2, axeC2, talA(2255-2492)/puf118(677-1036), pDM304, neoR | |
| [act15]:myoB | myoB overexpressed in AX3 | axeA1, axeB1, axeC1, myoB, [pDTb18], neoR | |
| [act15]:myoE(1-1005) | MyoE residues 1-1005 in AX2 (missing the TH1 domain) | axeA2, axeB2, axeC2, MyoE(1-1005), [pIS1], neoR | |
| [act15]:myoE(1-698(S336A)) | myoE (myosin IE) motor domain construct truncated at residue 698; the serine at the TEDS site was replaced by an alanine to mimic the unphosphorylated state | axeA1, axeB1, axeC1, pyr5-6-[pRG24], [Ddp2-ORF-pyr5-6], ura+[E698(S336A)], neoR | axe,neoR |
| [act15]:myoE(1-698(S336A)):2R | myoE (myosin IE) motor domain construct truncated at residue 698 and fused to an artificial lever arm consisting of two alpha-actinin repeats (2R); the serine at the TEDS site in the motor domain was replaced by an alanine to mimic the unphosphorylated state | axeA1, axeB1, axeC1, pyr5-6-[pRG24], [Ddp2-ORF-pyr5-6], ura+[E698(S336A)-2R], neoR | axe,neoR |
| [act15]:myoE(1-698(S336E)) | myoE (myosin IE) motor domain construct truncated at residue 698; the serine at the TEDS site was replaced by a glutamate to mimic the phosphorylated state | axeA1, axeB1, axeC1, pyr5-6-[pRG24], [Ddp2-ORF-pyr5-6], ura+ [E698(S336E)], neoR | axe, neoR |
| [act15]:myoE(1-698(S336E)):2R | myoE (myosin IE) motor domain construct truncated at residue 698 and fused to an artificial lever arm consisting of two alpha-actinin repeats (2R); the serine at the TEDS site of the motor domain was replaced by a glutamate to mimic the phosphorylated state | axeA1, axeB1, axeC1, pyr5-6-[pRG24], [Ddp2-ORF-pyr5-6], ura+[E698(S336A)-2R], neoR | axe,neoR |
| [act15]:myoE(1-698) | myoE (myosin IE) motor domain construct truncated at residue 698 | axeA1, axeB1, axeC1, pyr5-6-[pRG24], [Ddp2-ORF-pyr5-6], ura+[E698], neoR | axe,neoR |
| [act15]:myoE(1-698):2R | myoE (myosin IE) motor domain construct truncated at residue 698 and fused to an artificial lever arm consisting of two ?-actinins | axeA1, axeB1, axeC1, pyr5-6-[pRG24], [Ddp2-ORF-pyr5-6], ura+[E698-2R], neoR | axe,neoR |
| [act15]:myoE(K859A/K863A/R868A/R869A):GFP | C-terminal GFP-tagged myoE with K859A/K863A/R868A/R869A substitutions in AX2 | axeA2, axeB2, axeC2, myoE(K859A/K863A/R868A/R869A):GFP, [pIS1], neoR | |
| [act15]:myoE:YFP | C-terminal YFP to myosin-IE (myoE) fusion in wild-type | axeA1, axeB1, axeC1, pyr5-6-[pRG24], [Ddp2-ORF-pyr5-6], ura+ [myoE-EYFP], neoR | axe,neoR |
| [act15]:myoI:rasG(CTLL) | myoI with a C-terminal prenylation motif (RasG:CTLL) in AX2 | axeA2, axeB2, axeC2, myoI:rasG(CTLL), [pDTi346], neoR | |
| [act15]:myoK:Myc | wild type overexpressing C-terminally Myc-tagged myoK thus masking the Farnesyl (CLIQ) tail | axeA2, axeB2, axeC2, [act15]:myoK:Myc, neoR | |
| [act15]:myoM | overexpressor of myoM under the act15 promoter | axeA2, axeB2, axeC2, neoR, [act15]:myoM | |
| [act15]:myoM(1-892) | overexpressor of myoM head domain (1-892) under the act15 promoter | axeA2, axeB2, axeC2, neoR, -[act15]:myoM(1-892) | |
| [act15]:myoM(1-993) | overexpressor of myoM head domain (1-993) under the act15 promoter | axeA2, axeB2, axeC2, neoR, [act15]:myoM(1-993) | |
| [act15]:myoM-Mtail | overexpressor of myoM tail domain under the act15 promoter | axeA2, axeB2, axeC2, neoR, act15p-myoM-Mtail-OE | |
| [act15]:myr:rckA | overexpression of rckA with an N-terminal myristoylation
signal (from chicken c-Src) under the control of the constitutive act15 promoter | axeA1, axeB1, axeC1, [myr-rckA-OE], neoR | |
| [act15]:ndkC | overexpressor of ndkC under the control of the act15 promoter expressed in wild type | axeA2, axeB2, axeC2, act15:ndkC:GFP, neoR | |
| [act15]:nramp1:GFP | C-terminal nramp1-GFP fusion under the control of the constitutive act15 promoter | axeA2, axeB2, axeC2, neoR, [actin15-Nramp1::GFP] | |
| [act15]:nxnA:GFP | nxnA-GFP expressed in AX2; neomycin resistant | axeA2, axeB2, axeC2, nxnA-GFP, [pDdA15gfp], neoR | |
| [act15]:odc | odc overexpressed in KAX3 | axeA1, axeB1, axeC1, odc, [pEXP4(+)], neoR | |
| [act15]:odc:eYFP | C-terminal eYFP-marked odc overexpressed in AX2 | axeA2, axeB2, axeC2, odc:eYFP, [p[act15]:odc-Eyfp], neoR | |
| [act15]:p2xB:RFP/[act15]:vatM-GFP | AX2 overexpressing p2xB-RFP and vatM-GFP; co-transformed | axeA2, axeB2, axeC2, p2xB-RFP, vatM-GFP, neoR | |
| [act15]:p2xC:RFP/[act15]:vatM-GFP | AX2 overexpressing p2xC-RFP and vatM-GFP; co-transformed | axeA2, axeB2, axeC2, p2xC-RFP, vatM-GFP, neoR | |
| [act15]:p2xD:RFP/[act15]:vatM-GFP | AX2 overexpressing p2xD-RFP and vatM-GFP; co-transformed | axeA2, axeB2, axeC2, p2xD-RFP, vatM-GFP, neoR | |
| [act15]:p2xE:RFP/[act15]:vatM-GFP | AX2 overexpressing p2xE-RFP and vatM-GFP; co-transformed | axeA2, axeB2, axeC2, p2xE-RFP, vatM-GFP, neoR | |
| [act15]:pakA | overexpressor of pakA | axeA1, axeB1, axeC1, [act15]:pakA, neoR | |
| [act15]:pakA(K394A) | overexpressor of pakA(K394A) dominant negative mutant | axeA1, axeB1, axeC1, [act15]:pakA(K394A), neoR | |
| [act15]:pakA[CA] | overexpressor of pakA[CA] constitutively active mutant | axeA1, axeB1, axeC1, [act15]:pakA[CA], neoR | |
| [act15]:pakA[G+K] | overexpressor of pakA mutant containing the CRIB and kinase domains | axeA1, axeB1, axeC1, [act15]:pakA[G+K], neoR | |
| [act15]:pakB(1-337:563-852) | wild type AX2 cells overexpressing a constitutively active pakB kinase | axeA2, axeB2, axeC2, pakB(1-337:563-852), neoR | |
| [act15]:paxB:gfp | knock-in of paxB-GFP under the control of the act15 promoter | axeA2, axeB2, axeC2, [paxB/gfp/bsr], bsR | axe, bsR, gfp |
| [act15]:pde4(264-771):GFP | overexpression of the pde4 catalytic domain as a GFP fusion, neomycin resistant | axeA2, axeB2, axeC2, pde4cat-GFP[MB74-PDE4cat-GFP]neoR | |
| [act15]:pde4:GFP | overexpression of the pde4 gene, uracil auxotroph, neomycin resistant | axeA1, axeB1, axeC1, pyr5-6-[pRG24], ura-, [MB74-PDE4-GFP]neoR | |
| [act15]:pdeE:YFP | C-terminal YFP-tagged pdeE in AX2 | axeA2, axeB2, axeC2, pdeE:YFP, [pDXA-HC], neoR | |
| [act15]:phr2ab:GFP | 30133996 | axeA2, axeB2, axeC2, phr2ab:GFP, [p1ABsr8], bsR | |
| [act15]:pisA | pisA overexpressed in AX2 cells | axeA2, axeB2, axeC2, [act15]:pisA, bsR | |
| [act15]:pisA:GFP | pisA tagged with GFP overexpressed in AX2 | axeA2, axeB2, axeC2, pisA:GFP, bsR | |
| [act15]:pkaC | PkaC under control of act15 promotor over expressed in KAX3 | axeA1, axeB1, axeC1, pkaC, [BS18], neoR | |
| [act15]:pkaC(1-497) | overexpressor of pkaC missing 151 amino acids at its C terminus | axeA2, axeB2, axeC2, [act15]:pkaC(1-497), neoR | |
| [act15]:pkaC:HA | expressing PkaC under control of act15 promoter in AX4 | axeA1, axeB1, axeC1, [act15]:pkaC:HA, neoR | |
| [act15]:pkaR | pkaR overexpressor (agg-) | axeA2, axeB2, axeC2, [act15/pkaR], neoR | agg-,axe,neoR |
| [act15]:pkaR(G135E/G261A) | constitutively active pkaR (PKA regulatory subunit; point mutant (G135E/G261A) that is unable to bind cAMP | axeA2, axeB2, axeC2, pkaR(G135E/G261A) | |
| [act15]:pkaR(G135E/G261A)/AX3 | constitutively active pkaR (PKA regulatory subunit; point mutant (G135E/G261A) that is unable to bind cAMP in AX3 | axeA1, axeB1, axeC1, pkaR(G135E/G261A), [pA15-Rm], neoR | |
| [act15]:pkaR(S133T | pkaR in AX2 with S133T, G135E substitutions with reduce ability to bind cAMP | axeA2, axeB2, axeC2, pkaR(S133T, G135E), [pB10ActBKH], neoR | |
| [act15]:pkaR(S133T | pkaR with S133T, G135E substitutions expressing LacZ under control of act15 promotor | axeA2, axeB2, axeC2, pkaR(S133T, G135E), [pB10ActBKH, lacZ, [pAct15-Gal], neoR | |
| [act15]:pkcA | pkcA overexpressed in AX2 | axeA2.axeB2, axeC2, pkcA, neoR | |
| [act15]:pldB | AX2 cells overexpressing pldB under the control of the constitutive act15 promoter | axeA2, axeB2, axeC2, [A15pldB-neo], neoR | axe,neoR,agg- |
| [act15]:psaA:GFP | wildtype AX3 cells overexpressing psaA-GFP | axeA1, axeB1, axeC1, [act15]:psaA:GFP, neoR | |
| [act15]:psiA | psiA overexpressed in AX2 under control of act15 promotor | axeA2, axeB2, axeC2, psiA, [pEXP4(+)], neoR | |
| [act15]:psiA/[cudA]:lacZ | psiA overexpressed in AX2 under control of act15 promotor marked with lacZ under control of cudA promotor | axeA2, axeB2, axeC2, psiA, [pEXP4(+)], lacZ, neoR | |
| [act15]:psiA/[ecmB]:lacZ | psiA overexpressed in AX2 under control of act15 promotor marked with lacZ under control of ecmB promotor
| axeA2, axeB2, axeC2, psiA, [pEXP4(+)], lacZ, neoR | |
| [act15]:pten(16-515):GFP | GFP-tagged pten with deletion of residues 1-15 | axeA2, axeB2, axeC2, pten(16-515):GFP, [pJK1], neoR | |
| [act15]:pten(186-515):GFP | GFP-tagged pten with deletion of residues 1-185 in AX2 | axeA2, axeB2, axeC2, pten(186-515):GFP, [pJK1], neoR | |
| [act15]:pten(C124S):GFP | GFP-tagged pten with active site C124S substitution | axeA2, axeB2, axeC2, pten(C124S):GFP, [pJK1], neoR | |
| [act15]:pten(G129E):GFP | GFP-tagged pten with active site substitution G129E | axeA2, axeB2, axeC2, pten(G129E):GFP, [pJK1], neoR | |
| [act15]:rab11A:RFP | mutant overexpressing C-terminal rab11A-RFP; clone 3 | axeA1, axeB1, axeC1, rab11A:RFP, [pDM324], neoR | |
| [act15]:rac1B(Q61L) | constitutively active rac1B(Q61L) overexpressed in KAX3 | axeA1, axeB1, axeC1, rac1B(Q61L), neoR | |
| [act15]:rac1B(T17N) | dominant negative rac1B(T17N) overexpressed in KAX3 | axeA1, axeB1, axeC1, rac1B(T17N), neoR | |
| [act15]:racE(G20V):GFP | constitutively active RacE expressed in AX2-214 cells | axeA2, axeB2, axeC2, racE(G20V):GFP, [pDEX-GFP], neoR | |
| [act15]:rapA(G12V) | doxycycline-inducible, constitutively active RapA under the control of the act15 promoter in AX3 cells | axeA1, axeB1, axeC1, rapA(G12V), [pDM310], neoR | |
| [act15]:rasD(A118C):FLAG | Expressing FLAG-tagged rasD with an Ala to Cys exchange at position 1118 | axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, thy-, rasD(A118C):FLAG, neoR | |
| [act15]:rasD(Q61L) | constitutively active rasD(Q61L) expressed in KAX3 | axeA1, axeB1, axeC1, rasD(Q61L) | |
