| Strain Descriptor |
Strain Summary |
Genotype |
Phenotype |
| zak2-/[act15]:GFP | zak2 null mutant expressing GFP under the control of the actin 15 promoter | axeA1, axeB1, axeC1, zak2-, GFP, bsR | |
| zak2-/[ecmA]:LacZ | zak2 null cells expressing lacZ under control of the ecmA promoter | axeA1, axeB1, axeC1, zak2-, ecmA:lacZ, bsR | |
| zak2-/[ecmA]:zakA | zak2 null mutant expressing zakA under the control of the ecmA promoter | axeA1, axeB1, axeC1, zak2-, ecmA:zakA, bsR | |
| zak2-/[ecmB]:LacZ | zak2 null cells expressing lacZ under control of the ecmB promoter | axeA1, axeB1, axeC1, zak2-, ecmB:lacZ, bsR | |
| zakA- | zakA null in AX3 | axeA1, axeB1, axeC1, zakA-, bsR | axe,bsR |
| zakA- | zakA null mutant in AX2 | axeA2, axeB2, axeC2, zakA- , bsR | axe, bsR, tipped mounds |
| zakA-/[ecmA]:LacZ | zakA null cells expressing lacZ under control of the ecmA promoter | axeA1, axeB1, axeC1, zakA-, ecmA:lacZ, bsR | |
| zakA-/[ecmB]:LacZ | zakA null cells expressing lacZ under control of the ecmB promoter | axeA1, axeB1, axeC1, zakA-, ecmA/lacZ, bsR | |
| zfaA- | zfaA null mutant | axeA2, axeB2, axeC2, zfaA-, neoR | |
| zipA- | zipA REMI mutant | axeA1, axeB1, axeC1, zipA-, bsR | |
| zizA- | zizA null mutant expressed in AX2 | axeA2, axeB2, axeC2, zizA-, [bsR-loxP], bsr | |
| zizB- | zizB null mutant expressed in AX2 | axeA2, axeB2, axeC2, zizB-, [bsR-loxP], bsr | |
| zizD- | zizD REMI mutant; the insertion site is 510 bp downstream of the 5'' end of the second exon; insertion at the upper case NlaIII restriction site (CATG) in this sequence: acagtatcCATGacaccac;
get raw sequence data | axeA1, axeB1, axeC1, zizD-, bsR | |
| zntA- | zntA null mutant in AX2 | axeA2, axeB2, axeC2, zntA-, [pKOSG-IBA-dicty1], bsR | |
| zntB- | GWDI zntB null mutant in AX4 mutant | axeA1, axeB1, axeC1, zntB-, [pGWDI1], bsR | |
| [act15]:6xHis:amd1:eYFP | C-terminal eYFP-marked amd1 overexpressed in AX2 | axeA2, axeB2, axeC2, 6xHis:amd1:eYFP, [p[act15]:amd1-Eyfp], neoR | |
| [act15]:6xHis:ei24:eYFP | C-terminal YFP-tagged ei24 overexpressed in AX2 cells | axeA2, axeB2, axeC2, 6xHis:ei24:eYFP, [pacg-eYFP], neoR | |
| [act15]:6xHis:erkA | ErkA containing an N-terminal 6xHis tag overexpressed in KAX3 | axeA1, axeB1, axeC1, 6xHis:erkA, [pEXP4(+)], neoR | |
| [act15]:6xHis:racB/[tet]:tRNA-glu-CUC-1 | regulated expression of RacB in AX4 cells using an inducible tRNA suppressor gene | axeA1, axeB1, axeC1, GFP:abpC(1-250), [pDXA-HC], racB, [pDHRacB.UAB], [tet]:tRNA-glu-CUC-1, [pDNTS3], neoR, hygR | |
| [act15]:6xHis:sesn:eYFP | C-terminal YFP-tagged sesn under control of act15 promotor overexpressed in AX2 | axeA2, axeB2, axeC2, 6xHis:sesn:eYFP, neoR | |
| [act15]:7xHis:myoK:Ty1 | myoK overexpressed in AX2, containing an N-terminal 7xHis tag and a C-terminal Ty1 tag | axeA2, axeB2, axeC2, 7xHis:myoK:Ty1, bsR | |
| [act15]:8xHis:GFP:clmA | overexpressor of clmA (clathrin assembly protein AP180) His-tagged and fused to GFP, under the control of the constitutive act15 promoter | axeA2, axeB2, axeC2, [act15]:8xHis:GFP:clmA, neoR | |
| [act15]:abcA | acbA overexpressor in AX4 | axeA1, axeB1, axeC1, [A15::acbA/neo], neoR | axe,neoR |
| [act15]:abpB(129-295) | abpB residues 129-295 expressed in AX2 | axeA2, axeB2, axeC2, abpB(129-295), [pBORP], neoR | |
| [act15]:abpC:GFP | C-terminal fusion of GFP to full length abpC (ABP-120, ddFLN) expressed in wild type | axeA2, axeB2, axeC2, abpC:GFP, neoR | |
| [act15]:abpC:GFP | AbpC with C-terminal GFP in AX2-214 | axeA2, axeB2, axeC2, abpC, [p1ABsr8], bsR | |
| [act15]:abpD | abpD overexpressed in wild type | axeA2, axeB2, axeC2, abpD, neoR | |
| [act15]:abpE | abpE overexpressed in wild type; extrachromosomal vector; neomycin resistant | axeA2, axeB2, axeC2, abpE+[abpE-neo], neoR | axe, neoR |
| [act15]:acrA(1-731):YFP | YFP-marked AcrA C-terminally truncated after the HisKA domain expressed in wt | axe, matA1, acrA(1-731):YFP, neoR | |
| [act15]:act15(Y144W) | actin with a tyrosine for tryptophan substitution at residue 144 expressed in AX2 cells | axeA2, axeB2, axeC2, act15(Y144W), [pTIKL], neoR | |
| [act15]:adcA:GFP | adcA tagged with GFP overexpressed in KAX3 | axeA1, axeB1, axeC1, adcA:GFP, neoR | |
| [act15]:adprt1A | adprt1A overexpressed in AX2 | axeA2, axeB2, axeC2, adprt1A, neoR | |
| [act15]:amtA:GFP | amtA-GFP fusion under the control of the act15 promoter expressed in AX2 | axeA2, axeB2, axeC2, amtA:GFP, neoR | |
| [act15]:aprA | Overexpressor of aprA in AX2. | axeA2, axeB2, axeC2, actin15::aprA, neoR | axe, neoR |
| [act15]:aqpB:GFP | C-terminal GFP-AqpB fusion under the control of the act15 promoter expressed in wild type | axeA2, axeB2, axeC2, aqpB:GFP, neoR | |
| [act15]:arcB:TAP | arcB fused to TAP tag for tandem affinity purification expressed under the control of the constitutive act15 promoter | axeA2, axeB2, axeC2, [act15/arcB-TAP], neoR | |
| [act15]:atg1 | wild type cells overexpressing atg1 | axeA1, axeB1, axeC1, pyr5-6-[pRG24], ura-, atg1, neoR | |
| [act15]:atg1(1-628) | wild type expressing atg1 lacking the C-terminal 40 amino acids | axeA1, axeB1, axeC1, pyr5-6-[pRG24], ura-, atg1(1-628), neoR | |
| [act15]:atg1(K36A) | wild type expressing atg1 with a K to A mutation, which renders the kinase inactive; acts as a dominant-negative | axeA1, axeB1, axeC1, pyr5-6-[pRG24], ura-, atg1(K36A), neoR | |
| [act15]:atg1(K36A/1-628) | wild type expressing atg1 with a K to A mutation at position 36 that renders the kinase inactive and lacking the C-terminal 40 amino acids; does not act as a dominant-negative like the expression of K36A alone | axeA1, axeB1, axeC1, pyr5-6-[pRG24], ura-, atg1(K36A/1-628), neoR | |
| [act15]:badA | badA overexpressed in DH1-10 | axeA1, axeB1, axeC1, pyr5-6-[pRG24], ura-, badA, [pDXA-3C], neoR | |
| [act15]:bzpN:GFP | C-terminal fusion of GFP to bzpN under the control of the actin15 promoter expressed in wild type AX4 cells | axeA1, axeB2, axeC2, [act15]:GFP, neoR | |
| [act15]:cadA | CadA overexpressed in KAX3 cellls | axeA1, axeB1, axeC1, cadA, [pEXP4(+)], bsR | |
| [act15]:cak1:myc | myc-tagged cak1 expressed under the control of the actin 15 promoter; neomycin resistant | axeA2, axeB2, axeC2, [act15]:cak1, neoR | |
| [act15]:calA:FLAG | mutant expressing FLAG-tagged calA | axeA1, axeB1, axeC1, act15:calA:FLAG, neoR | |
| [act15]:carA | cAR1 overexpressor in AX3 | axeA1, axeB1, axeC1, [pB18-carA], neoR | |
| [act15]:carA(I104A) | overexpressor of carA with Ile 104 replaced by Ala | axeA1, axeB1, axeC1, neoR, [act15/carA(I104A)] | |
| [act15]:carA(I104C) | overexpressor of carA with Ile 104 replaced by Cys | axeA1, axeB1, axeC1, neoR, [act15/carA(I104C)] | |
| [act15]:carA(I104D) | overexpressor of carA with Ile 104 replaced by Asp | axeA1, axeB1, axeC1, neoR, [act15/carA(I104D)] | |
| [act15]:carA(I104E) | overexpressor of carA with Ile 104 replaced by Glu | axeA1, axeB1, axeC1, neoR, [act15/carA(I104E)] | |
| [act15]:carA(I104F) | overexpressor of carA with Ile 104 replaced by Phe | axeA1, axeB1, axeC1, neoR, [act15/carA(I104F)] | |
| [act15]:carA(I104G) | overexpressor of carA with Ile 104 replaced by Gly | axeA1, axeB1, axeC1, neoR, [act15/carA(I104G)] | |
| [act15]:carA(I104H) | overexpressor of carA with Ile 104 replaced by His | axeA1, axeB1, axeC1, neoR, [act15/carA(I104H)] | |
| [act15]:carA(I104K) | overexpressor of carA with Ile 104 replaced by Lys | axeA1, axeB1, axeC1, neoR, [act15/carA(I104K)] | |
| [act15]:carA(I104L) | overexpressor of carA with Ile 104 replaced by Leu | axeA1, axeB1, axeC1, neoR, [act15/carA(I104L)] | |
| [act15]:carA(I104M) | overexpressor of carA with Ile 104 replaced by Met | axeA1, axeB1, axeC1, neoR, [act15/carA(I104M)] | |
| [act15]:carA(I104N) | overexpressor of carA with Ile 104 replaced by Asn | axeA1, axeB1, axeC1, neoR, [act15/carA(I104N)] | |
| [act15]:carA(I104P) | overexpressor of carA with Ile 104 replaced by Pro | axeA1, axeB1, axeC1, neoR, [act15/carA(I104P)] | |
| [act15]:carA(I104Q) | overexpressor of carA with Ile 104 replaced by Gln | axeA1, axeB1, axeC1, neoR, [act15/carA(I104Q)] | |
| [act15]:carA(I104R) | overexpressor of carA with Ile 104 replaced by Arg | axeA1, axeB1, axeC1, neoR, [act15/carA(I104R)] | |
| [act15]:carA(I104S) | overexpressor of carA with Ile 104 replaced by Ser | axeA1, axeB1, axeC1, neoR, [act15/carA(I104S)] | |
| [act15]:carA(I104T) | overexpressor of carA with Ile 104 replaced by Thr | axeA1, axeB1, axeC1, neoR, [act15/carA(I104T)] | |
| [act15]:carA(I104V) | overexpressor of carA with Ile 104 replaced by Val | axeA1, axeB1, axeC1, neoR, [act15/carA(I104V)] | |
| [act15]:carA(I104W) | overexpressor of carA with Ile 104 replaced by Trp | axeA1, axeB1, axeC1, neoR, [act15/carA(I104W)] | |
| [act15]:carA(I104Y) | overexpressor of carA with Ile 104 replaced by Tyr | axeA1, axeB1, axeC1, neoR, [act15/carA(I104Y)] | |
| [act15]:carB | AX4 expressing cAR2 (carB) from the actin 15 promoter. | axeA1, axeB1, axeC1, [pBS18::gcAR2], neoR | axe, neoR |
| [act15]:catA | catA overexpressed in KAX3
| axeA1, axeB1, axeC1, catA, [pExp4(+)], neoR | |
| [act15]:cdcD(E219K):RFP | RFP-marked cdcD with an E219K substitution in AX2 | axeA2, axeB2, axeC2, cdcD(E219K):RFP, neoR | |
| [act15]:cdcD(R154C):RFP | RFP-marked cdcD with an R154C substitution in AX2; in human ortholog R155C mutation causes IBMPFD (inclusion body myopathy with early onset Paget''''s disease of bone and frontotemporal dementia) | axeA2, axeB2, axeC2, cdcD(R154C):RFP, neoR | |
| [act15]:cdcD:RFP | RFP-marked cdcD in AX2 | axeA2, axeB2, axeC2, cdcD:RFP, neoR | |
| [act15]:cdk5(D144N) | overexpressor of a dominant negative mutant of cdk5(D144N) under the control of the constitutive act15 promoter | [act15]:cdk5-D144N, axeA2, axeB2, axeC2 | |
| [act15]:cdk5:GFP | wild type (AX3) overexpressing cdk5-GFP | axeA1, axeB1, axeC1, [act15]:cdk5:GFP, neoR | |
| [act15]:cf60 | overexpression of cf60 from the actin 15 promoter; neomycin resistant | axeA2, axeB2, axeC2, [cf60OE], neoR | |
| [act15]:cfaD | Overexpressor of cfaD in AX2 | axeA2, axeB2, axeC2, [actin15::cfaD], neoR | axe, neoR |
| [act15]:CFP:racC(G15V) | overexpressor of racC(G15V), a constitutively active mutant, fused with cyan fluorescence protein | axeA1, axeB1, axeC1, [act15]:CFP:racC(G15V), neoR | |
| [act15]:CFP:racC(T20N) | overexpressor of racC(T20N), a dominant negative mutant, fused with cyan fluorescence protein | axeA1, axeB1, axeC1, [act15]:CFP:racC(T20N), neoR | |
| [act15]:cnrF:GFP | C-terminal fusion of GFP to cnrF under the control of the act15 promoter expressed in AX4 background | axeA1, axeB1, axeC1, [act15:cnrF:GFP], neoR | |
| [act15]:cnrN:myc/smlA- | overexpressor C-terminal myc-tagged cnrN under the control of the act15 promoter in smlA null mutant | axeA1, axeB1, axeC1, pyr5-6-[pRG24], ura+, smlA-[pSmlA-KO], act15:cnrN:myc, [pDXA-3D], neoR | |
| [act15]:cnxA:GFP | calnexin-GFP fusion | axeA2, axeB2, axeC2, [calnexin-GFP in pDEXRH], neoR | wt,axe,neoR |
| [act15]:cofA | cofA under the control of the actin15 promoter overexpressed in wild type | axeA2, axeB2, axeC2, cofA[pCOF], neoR | |
| [act15]:csaA | csaA over expressed in vegetative cells under control of the act15 promoter using the pDCEV vector | axeA2, axeB2, axeC2, csaA, [pDCEV], neoR | |
| [act15]:csaA(1-485):gppA(470-544):GFP | csA/dajumin-GFP expressing strain | axeA2, axeB2, axeC2, csaA/dajumin-GFP, [pDEXH], neoR | axe, neoR, gfp |
| [act15]:csaA(1-488):gppA(469-544) | CsaA without C-terminal GPI-anchor signal, but including TMM region from gppA under control of act15 promotor | axeA2, axeB2, axeC2, csaA(1-488):gppA(469-544), [pDEXRH], neoR | |
| [act15]:csaA(1-504):gppA(488-519):p80(431-481) | CsaA protein with a gppA (P29F8) transmembrane domain fused to the cytoplasmic domain of p80 | axeA1, axeB1, axeC1, pyr5-6-[pRG24], ura-, csaA-gppA[pDCEV4-CP], p80[pDCEV4-CP-p80]neoR | |
| [act15]:cynA:GFP | GFP-tagged cynA in AX3.1 | axeA1, axeB1, axeC1, cynA:GFP, [pJK1], neoR | |
| [act15]:cyrA:GFP | wild type (AX3) overexpressing cyrA-GFP | axeA1, axeB1, axeC1, [act15]:cyrA:GFP, neoR | |
| [act15]:dagA | dagA overexpressor in AX4 background | axeA1, axeB1, axeC1, [act15]:dagA, neoR | |
| [act15]:dagA(1-233):GFP(S65T) | N-terminal, PH domain of CRAC tagged with GFP(S65T) expressed in AX3 cells | axeA1, axeB1, axeC1, dagA(1-233), [PH-GFP], neoR | |
| [act15]:DD:GFP:rab8A(N128I) | dominant-negative rab8A mutation fused to DD:GFP, expressed in wild type; Rab8a fused to C-terminus of the destabilizing domain (DD, 107 residues), which causes rapid degradation of the DD fusion protein; addition of the compound Shield-1 (Shld1) rapidly and reversibly stabilizes it . | axeA2, axeB2, axeC2, DD:GFP:rab8A(N128I), [pDXA-DD-GFP], neoR | |
| [act15]:DD:GFP:rab8A(Q74L) | constitutively active rab8A mutation fused to DD:GFP, expressed in wild type; Rab8a fused to C-terminus of the destabilizing domain (DD, 107 residues), which causes rapid degradation of the DD fusion protein; addition of the compound Shield-1 (Shld1) rapidly and reversibly stabilizes it . | axeA2, axeB2, axeC2, DD:GFP:rab8A(Q74L), [pDXA-DD-GFP], neoR | |
| [act15]:DDB_G0272656 | overexpressor of sigN4 (DDB_G0272656) under the control of the act15 promoter | axeA1, axeB1, axeC1, [act15]:DDB_G0272656, neoR | |
| [act15]:deeJ | deeJ (DJ-1) overexpressed in AX2 | axeA2, axeB2, axeC2, deeJ, [pPROF690], neoR | |
| [act15]:dgkA | dgkA under the control of the actin 15 promoter expressed in wild type; neomycin resistant | axeA2, axeB2, axeC2, dgkA-[pDRE106], neoR | |
| [act15]:dhcA(1384-4725) | C-terminal fragment (residues 1384-4725) expressed in AX2 | axeA2, axeB2, axeC2, dhcA(1384-4725), [pBORP], neoR | |
| [act15]:dhkC(394-661) | kAX3 cells expressing the dhkC histidine kinase domain under the control of the constitutively active act15 promoter | axeA1, axeB1, axeC1, neoR, [act15]:dhkC(394-661) | |
| [act15]:dhkK | AX2 cells overexpressing dhkK under the control of the constitutive act15 promoter | axeA2, axeB2, axeC2, [act15/dhkK] | |
| [act15]:dhkK(D1125N) | AX2 cells overexpressing dhkK with a mutation in the RR aspartate phospho-acceptor site (D1125N) | axeA2, axeB2, axeC2, [act15/dhkK(D1125N)] | |
| [act15]:dhkK(H825Q) | AX2 cells overexpressing dhkK with a mutation in the HK histidine phospho-acceptor site (H825Q) | axeA2, axeB2, axeC2, [act15/dhkK(H825Q)] | |
| [act15]:dhkK(H825Q/D1125N) | AX2 cells overexpressing dhkK with mutations in the HK histidine phospho-acceptor site (H825Q) and in the RR aspartate phospho-acceptor site (D1125N) | axeA2, axeB2, axeC2, [act15/dhkK(H825Q/D1125N)], bsR | |
| [act15]:dimA:GFP | expression of dimA-GFP under the control of the act15 promoter | axeA1, axeB1, axeC1, dimA:GFPl, neoR | |
